RTG signaling sustains mitochondrial respiratory capacity in HOG1-dependent osmoadaptation
Open accessAbstract
Mitochondrial RTG-dependent retrograde signaling, whose regulators have been characterized in Saccharomyces cerevisiae, plays a recognized role under various environmental stresses. Of special significance, the activity of the transcriptional complex Rtg1/3 has been shown to be modulated by Hog1, the master regulator of the high osmolarity glycerol pathway, in response to osmotic stress. The present work focuses on the role of RTG signaling in salt-induced osmotic stress and its interaction with HOG1. Wild-type and mutant cells, lacking HOG1 and/or RTG genes, are compared with respect to cell growth features, retrograde signaling activation and mitochondrial function in the presence and in the absence of high osmostress. We show that RTG2, the main upstream regulator of the RTG pathway, contributes to osmoadaptation in an HOG1-dependent manner and that, with RTG3, it is notably involved in a late phase of growth. Our data demonstrate that impairment of RTG signaling causes a decrease in mitochondrial respiratory capacity exclusively under osmostress. Overall, these results suggest that HOG1 and the RTG pathway may interact sequentially in the stress signaling cascade and that the RTG pathway may play a role in inter-organellar metabolic communication for osmoadaptation.
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Interactions between mitochondria and peroxisomes were revealed in studies of the HOG-dependent response to hyperosmotic stress in S. cerevisiae that also involves the Rtg pathway.
“Interactions between mitochondria and peroxisomes were revealed in the studies of HOG-dependent response to the hyperosmotic stress in S. cerevisiae that also involves the Rtg pathway (Guaragnella et al. 2021; Di Noia et al. 2023).”
From Mitochondrial retrograde control of transcription evolves with respiratory stress, metabolic adaptation, and virulence in budding yeasts (2026) · cites this paper as Guaragnella et al. 2021
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- Authors
- Guaragnella, N., Agrimi, G., Scarcia, P., Suriano, C., Pisano, I., Bobba, A., Mazzoni, C., Palmieri, L., & Giannattasio, S.
- Affiliations
- Institute of Biomembranes, Bioenergetics and Molecular Biotechnologies (IT)
- University of Bari Aldo Moro (IT)
- Istituto Pasteur (IT)
- Corresponding author
- Guaragnella, Nicoletta
- ORCID
- Venue
- Microorganisms
- Publisher
- MDPI AG
- Published
- 2021-09-06
- Volume
- 9
- Issue
- 9
- Pages
- 1894
- ISSN
- 2076-2607 (ISSN-L)
- PMC
- PMC8466848
- OpenAlex
- W3197001066
- Cited by
- 16
- References
- 30
- Citations by year
- 2026: 5 · 2025: 3 · 2024: 4 · 2023: 2 · 2022: 2
- FWCI
- 0.63
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Microorganisms (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, other-oa)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- IRIS Research product catalog (Sapienza University of Rome) (submittedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- CINECA IRIS Institutional Research Information System (University of Bari Aldo Moro) (submittedVersion)
- Funders
- European Regional Development Fund (FSE 2014-2020, 2014-2020’)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- retrograde signaling, mitochondrial respiratory capacity, Hog1, osmoadaptation, osmotic stress, salt stress, Saccharomyces cerevisiae
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