Cited by 30Endometriosis Research and TreatmentEctopic Pregnancy Diagnosis and ManagementProtease and Inhibitor Mechanisms

Matrix Metalloproteinase-3 mRNA: A Promising Peripheral Blood Marker for Diagnosis of Endometriosis

De Sanctis, Paola, Elmakky, Amira, Farina, Antonio, Caramelli, Elisabetta, Seracchioli, Renato, Mabrouk, Mohamed, Mignemi, Giuseppe, Venturoli, Stefano +6 more·2011·Gynecologic and Obstetric Investigation

Abstract

Background/Aims: Endometriosis is an invasive disease. Its diagnosis depends on laparoscopy, which is traumatic and associated with potential complications. The aim of this study was to develop a rapid, reliable, and less invasive diagnostic test for endometriosis. We hypothesized that genes related to cell invasion would be transcriptionally upregulated in endometriosis, and tested whether blood levels of their transcripts might be used as biomarkers of endometriosis.Methods:We used quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) to quantify the mRNA levels of vascular endothelial growth factor A (VEGFA), matrix metalloproteinase-3 (MMP-3), and MMP-9 in peripheral blood from 20 patients with mild/intermediate endometriosis, 20 patients with severe endometriosis and 20 endometriosis-free subjects. Results:Our results indicate that circulating mRNA for MMP-3 is significantly higher in patients with endometriosis than in control patients, regardless of the degree of severity. Conversely, the level of circulating mRNA for VEGFA and MMP-9 did not distinguish patients from controls. Conclusion: MMP-3 mRNA is a promising peripheral blood marker that discriminates between patients with endometriosis and healthy subjects. Our results support the possibility of finding genes suitable for diagnostic qRT-PCR for endometriosis in peripheral blood and should be explored further.

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Crossref · 10.1159/000320752

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38 of 38 references verified0 citation errors · 0 not checkable

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  • The same study showed similar levels of MMP-9 and VEGF-A mRNA among cases and controls.

    “The same study showed similar levels of MMP-9 and vascular endothelial growth factor A (VEGF-A) mRNA among cases and controls [66].”

    From Update on biomarkers for the detection of endometriosis (2015) · cites this paper as [66]

  • In de Sanctis et al., mRNA levels of MMP-3 were significantly higher in stage II-IV endometriosis cases than in controls.

    “In a study by de Sanctis et al., mRNA levels of MMP-3 were significantly higher in stage II–IV endometriosis cases than in controls [66].”

    From Update on biomarkers for the detection of endometriosis (2015) · cites this paper as [66]

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Authors
De Sanctis, P., Elmakky, A., Farina, A., Caramelli, E., Seracchioli, R., Mabrouk, M., Mignemi, G., Venturoli, S., Villa, G., Guerrini, M., Manuzzi, L., Montanari, G., Valvassori, L., & Zucchini, C.
Affiliations
  • University of Bologna (IT)
  • IRCCS Azienda Ospedliero-Universitaria di Bologna Policlinico di Sant'Orsola (IT)
Venue
Gynecologic and Obstetric Investigation
Publisher
S. Karger AG
Published
2010-12-09
Volume
71
Issue
2
Pages
118-123
ISSN
  • 0378-7346 (ISSN-L)
  • 1423-002X
OpenAlex
W2063425267
Cited by
30
References
38
Citations by year
2026: 1 · 2024: 1 · 2022: 2 · 2021: 2 · 2020: 2 · 2019: 2 · 2018: 4 · 2017: 1 · 2016: 3 · 2015: 4 · 2014: 4 · 2012: 4
FWCI
1.51
Type
journal article
Language
en
Open access
closed
Locations
  • Gynecologic and Obstetric Investigation (publishedVersion)
  • PubMed (publishedVersion)
  • Archivio istituzionale della ricerca (Alma Mater Studiorum Università di Bologna) (submittedVersion)
Topics
  • Health Sciences › Medicine › Reproductive Medicine › Endometriosis Research and Treatment
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Ectopic Pregnancy Diagnosis and Management
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Protease and Inhibitor Mechanisms
Keywords
endometriosis, matrix metalloproteinase-3, peripheral blood biomarkers, non-invasive diagnosis, VEGF-A, MMP-9, circulating mRNA, endometriosis severity
MeSH terms
Adult, Endometriosis, Female, Humans, RNA, Messenger, Biomarkers, Matrix Metalloproteinase 3, Reverse Transcriptase Polymerase Chain Reaction, Matrix Metalloproteinase 9, Vascular Endothelial Growth Factor A

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