Effect of L-Arginine and Selenium Added to a Hypocaloric Diet Enriched with Legumes on Cardiovascular Disease Risk Factors in Women with Central Obesity: A Randomized, Double-Blind, Placebo-Controlled Trial
Abstract
Background/aims We aimed to discover if L-arginine and selenium alone or together can increase the effect of a hypocaloric diet enriched in legumes (HDEL) on central obesity and cardiovascular risk factors in women with central obesity. Methods This randomized, double-blind, placebo-controlled trial was undertaken in 84 premenopausal women with central obesity. After a 2-week run-in period on an isocaloric diet, participants were randomly assigned to a control diet (HDEL), L-arginine (5 g/day) and HDEL, selenium (200 μg/day) and HDEL or L-arginine, selenium and HDEL for 6 weeks. Cardiovascular risk factors were assessed before intervention and 3 and 6 weeks afterwards. Results After 6 weeks, L-arginine had significantly reduced waist circumference (WC); selenium had significantly lowered fasting concentrations of serum insulin and the homeostasis model assessment of insulin resistance index; the interaction between L-arginine and selenium significantly reduced the fasting concentration of nitric oxides (NO(x)), and HDEL lowered triglycerides (TG) and WC and significantly increased the fasting concentration of NO(x). HDEL reduced high-sensitivity C-reactive protein levels in the first half of the study and returned them to basal levels in the second half. Conclusion These data indicate the beneficial effects of L-arginine on central obesity, selenium on insulin resistance and HDEL on serum concentrations of NO(x) and TG.
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Human studies have repeatedly shown that L-arginine supplementation has favorable effects on body composition and insulin sensitivity.
“However, human studies have repeatedly shown that L-arginine supplementation has favorable effects on body composition and insulin sensitivity [271-277].”
From Regulation of obesity and insulin resistance by nitric oxide (2014) · cites this paper as [273]
Cites
Matthews, D. R., Hosker, J. P., Rudenski, A. S., Naylor, B. A., Treacher, D. F., & Turner, R. C. (1985) · Diabetologia · doi:10.1007/bf00280883 · Cited by 31,502 · see citing works
Grundy, S. M., Bilheimer, D. W., Chait, A., Clark, L. T., Denke, M. A., Havel, R. J., Hazzard, W. R., Hulley, S. B., Hunninghake, D. B., Kreisberg, R. A., et al. (1993) · JAMA · doi:10.1001/jama.269.23.3015 · Cited by 27,944 · see citing works
The Ferric Reducing Ability of Plasma (FRAP) as a Measure of “Antioxidant Power”: The FRAP Assay
Benzie, I. F. F., & Strain, J. (1996) · Analytical Biochemistry · doi:10.1006/abio.1996.0292 · Cited by 23,027 · see citing works
Atherosclerosis--an inflammatory disease
Ross, R. (1999) · New England Journal of Medicine · doi:10.1056/nejm199901143400207 · Cited by 21,725 · see citing works
Trumbo, P., Schlicker, S., Yates, A. A., & Poos, M. (2002) · Journal of the American Dietetic Association · doi:10.1016/s0002-8223(02)90346-9 · Cited by 5,993 · see citing works
Grundy, S. M., Brewer, H. B., Cleeman, J. I., Smith, S. C., & Lenfant, C. (2004) · Circulation · doi:10.1161/01.cir.0000111245.75752.c6 · Cited by 5,982 · see citing works
A Rapid, Simple Spectrophotometric Method for Simultaneous Detection of Nitrate and Nitrite
Miranda, K. M., Espey, M. G., & Wink, D. A. (2001) · Nitric Oxide · doi:10.1006/niox.2000.0319 · Cited by 3,858 · see citing works
Arginine metabolism: nitric oxide and beyond
WU, G., & MORRIS, S. M. (1998) · Biochemical Journal · doi:10.1042/bj3360001 · Cited by 2,811 · see citing works
Free radicals, antioxidants, and nutrition
Fang, Y.-Z., Yang, S., & Wu, G. (2002) · Nutrition · doi:10.1016/s0899-9007(02)00916-4 · Cited by 2,568 · see citing works
A simple fluorometric assay for lipoperoxide in blood plasma
Yagi, K. (1976) · Biochemical Medicine · doi:10.1016/0006-2944(76)90049-1 · Cited by 2,115 · see citing works
Total Antioxidant Capacity of Fruits
Wang, H., Cao, G., & Prior, R. L. (1996) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf950579y · Cited by 1,686 · see citing works
Landmesser, U., Dikalov, S., Price, S. R., McCann, L. A., Fukai, T., Holland, S. M., Mitch, W. E., & Harrison, D. G. (2003) · Journal of Clinical Investigation · doi:10.1172/jci14172 · Cited by 1,652 · see citing works
Arginine metabolism and nutrition in growth, health and disease
Wu, G., Bazer, F. W., Davis, T. A., Kim, S. W., Li, P., Marc Rhoads, J., Carey Satterfield, M., Smith, S. B., Spencer, T. E., & Yin, Y. (2009) · Amino Acids · doi:10.1007/s00726-008-0210-y · Cited by 1,362 · see citing works
International Day for the Evaluation of Abdominal Obesity (IDEA)
Balkau, B., Deanfield, J. E., Després, J., Bassand, J., Fox, K. A. A., Smith, S. C., Barter, P. J., Tan, C. T., Gaal, L. V., Wïttchen, H., et al. (2007) · Circulation · doi:10.1161/circulationaha.106.676379 · Cited by 731 · see citing works
Regulatory role for the arginine–nitric oxide pathway in metabolism of energy substrates
Jobgen, W. S., Fried, S. K., Fu, W. J., Meininger, C. J., & Wu, G. (2006) · The Journal of Nutritional Biochemistry · doi:10.1016/j.jnutbio.2005.12.001 · Cited by 713 · see citing works
Mollnau, H., Wendt, M., Szöcs, K., Lassègue, B., Schulz, E., Oelze, M., Li, H., Bodenschatz, M., August, M., Kleschyov, A. L., et al. (2002) · Circulation Research · doi:10.1161/01.res.0000012569.55432.02 · Cited by 676 · see citing works
On the nature of selenium toxicity and carcinostatic activity
Spallholz, J. E. (1994) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(94)90007-8 · Cited by 631 · see citing works
The Metabolic Syndrome: Inflammation, Diabetes Mellitus, and Cardiovascular Disease
Haffner, S. M. (2005) · The American Journal of Cardiology · doi:10.1016/j.amjcard.2005.11.010 · Cited by 563 · see citing works
The diverse role of selenium within selenoproteins: a review.
Holben, D., & Smith, A. M. (1999) · Journal of the American Dietetic Association · doi:10.1016/s0002-8223(99)00198-4 · Cited by 443 · see citing works
Dietary Patterns and Markers of Systemic Inflammation among Iranian Women
Esmaillzadeh, A., Kimiagar, M., Mehrabi, Y., Azadbakht, L., Hu, F. B., & Willett, W. C. (2007) · The Journal of Nutrition · doi:10.1093/jn/137.4.992 · Cited by 405 · see citing works
Esteghamati, A., Meysamie, A., Khalilzadeh, O., Rashidi, A., Haghazali, M., Asgari, F., Kamgar, M., Gouya, M., & Abbasi, M. (2009) · BMC Public Health · doi:10.1186/1471-2458-9-167 · Cited by 388 · see citing works
Dietary L-arginine supplementation reduces fat mass in Zucker diabetic fatty rats
Fu, W. J., Haynes, T. E., Kohli, R., Hu, J., Shi, W., Spencer, T. E., Carroll, R. J., Meininger, C. J., & Wu, G. (2005) · The Journal of Nutrition · doi:10.1093/jn/135.4.714 · Cited by 353 · see citing works
RESPONSIVENESS OF SELENOPROTEINS TO DIETARY SELENIUM,
Allan, C. B., Lacourciere, G. M., & Stadtman, T. C. (1999) · Annual Review of Nutrition · doi:10.1146/annurev.nutr.19.1.1 · Cited by 351 · see citing works
The implication of obesity and central fat on markers of chronic inflammation: The ATTICA study
PANAGIOTAKOS, D., PITSAVOS, C., YANNAKOULIA, M., CHRYSOHOOU, C., & STEFANADIS, C. (2005) · Atherosclerosis · doi:10.1016/j.atherosclerosis.2005.03.010 · Cited by 340 · see citing works
Tan, B., Yin, Y., Liu, Z., Xin-guo, L., Xu, H.-J., Kong, X., Huang, R., Tang, W., Shinzato, I., Smith, S. B., et al. (2008) · Amino Acids · doi:10.1007/s00726-008-0148-0 · Cited by 309 · see citing works
Selenium, oxidative stress, and health aspects
Brenneisen, P., Steinbrenner, H., & Sies, H. (2005) · Molecular Aspects of Medicine · doi:10.1016/j.mam.2005.07.004 · Cited by 299 · see citing works
Jobgen, W., Meininger, C. J., Jobgen, S. C., Li, P., Lee, M.-J., Smith, S. B., Spencer, T. E., Fried, S. K., & Wu, G. (2009) · The Journal of Nutrition · doi:10.3945/jn.108.096362 · Cited by 288 · see citing works
Bartosz, G. (2003) · Advances in clinical chemistry · doi:10.1016/s0065-2423(03)37010-6 · Cited by 286 · see citing works
Kuhlencordt, P. J., Chen, J., Han, F., Astern, J., & Huang, P. L. (2001) · Circulation · doi:10.1161/01.cir.103.25.3099 · Cited by 252 · see citing works
Lucotti, P., Setola, E., Monti, L. D., Galluccio, E., Costa, S., Sandoli, E. P., Fermo, I., Rabaiotti, G., Gatti, R., & Piatti, P. (2006) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00002.2006 · Cited by 249 · see citing works
<scp>l</scp> -Arginine Supplementation in Peripheral Arterial Disease: No Benefit and Possible Harm
Wilson, A. M., Harada, R., Nair, N., Balasubramanian, N., & Cooke, J. P. (2007) · Circulation · doi:10.1161/circulationaha.106.683656 · Cited by 241 · see citing works
Role of Selenium in the Nutrition of the Chick
Thompson, J. N., & Scott, M. L. (1969) · Journal of Nutrition · doi:10.1093/jn/97.3.335 · Cited by 222 · see citing works
Detmers, P. A., Hernandez, M., Mudgett, J. S., Hassing, H., Burton, C. A., Mundt, S. S., Chun, S. H., Fletcher, D. S., Card, D., Lisnock, J., et al. (2000) · The Journal of Immunology · doi:10.4049/jimmunol.165.6.3430 · Cited by 206 · see citing works
Cheung, M. C., Zhao, X.-Q., Chait, A., Albers, J. J., & Brown, B. G. (2001) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/hq0801.095151 · Cited by 191 · see citing works
Acid-volatile selenium formation catalyzed by glutathione reductase
Hsieh, H., & Ganther, H. E. (1975) · Biochemistry · doi:10.1021/bi00679a014 · Cited by 185 · see citing works
Measurement of total antioxidant capacity
Young, I. S. (2001) · Journal of Clinical Pathology · doi:10.1136/jcp.54.5.339 · Cited by 180 · see citing works
Prabhu, K. S., Zamamiri-Davis, F. A., STEWART, J. B., et al. (2002) · Biochemical Journal · doi:10.1042/bj20020256 · Cited by 155 · see citing works
Kim, S. H., Johnson, V. J., Shin, T., et al. (2004) · Experimental Biology and Medicine · doi:10.1177/153537020422900209 · Cited by 150 · see citing works
Glutathione oxidase activity of selenocystamine: A mechanistic study
Chaudière, J., Courtin, O., & Leclaire, J. (1992) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(92)90580-p · Cited by 146 · see citing works
Wu, G., Flynn, N. E., Flynn, S. P., Jolly, C. A., & Davis, P. K. (1999) · Journal of Nutrition · doi:10.1093/jn/129.7.1347 · Cited by 144 · see citing works
Martina, V., Masha, A., Gigliardi, V. R., Brocato, L., Manzato, E., Berchio, A., Massarenti, P., Settanni, F., Casa, L. D., Bergamini, S., et al. (2008) · Diabetes Care · doi:10.2337/dc07-2251 · Cited by 137 · see citing works
O’Brien, K. D., Brehm, B. J., Seeley, R. J., Bean, J. A., Wener, M. H., Daniels, S. R., & D’Alessio, D. A. (2005) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2004-1011 · Cited by 130 · see citing works
Olszanecka‐Glinianowicz, M., Zahorska‐Markiewicz, B., Janowska, J., & Żurakowski, A. (2004) · Metabolism · doi:10.1016/j.metabol.2004.07.001 · Cited by 128 · see citing works
Lucotti, P., Monti, L. D., Setola, E., Canna, G. L., Castiglioni, A., Rossodivita, A., Pala, M. G., Formica, F., Paolini, G., Catapano, A. L., et al. (2009) · Metabolism · doi:10.1016/j.metabol.2009.03.029 · Cited by 116 · see citing works
Liu, T.-H., Wu, C.-L., Chiang, C.-W., Lo, Y.-W., Tseng, H.-F., & Chang, C.-K. (2009) · The Journal of Nutritional Biochemistry · doi:10.1016/j.jnutbio.2008.05.005 · Cited by 101 · see citing works
Haruna, Y., Morita, Y., Komai, N., Yada, T., Sakuta, T., Tomita, N., Fox, D. A., & Kashihara, N. (2006) · Arthritis & Rheumatism · doi:10.1002/art.21891 · Cited by 83 · see citing works
Faure, P., Ramon, O., Favier, A. E., & Halimi, S. (2004) · European Journal of Clinical Investigation · doi:10.1111/j.1365-2362.2004.01362.x · Cited by 81 · see citing works
Shen, C., Song, W. C., & Pence, B. C. (2001) · PubMed · Cited by 70 · see citing works
Redlich, C. A., Chung, J. S., Cullen, M. R., Blaner, W. S., van Bennekum, A. M., & Berglund, L. (1999) · Atherosclerosis · doi:10.1016/s0021-9150(99)00266-x · Cited by 58 · see citing works
Belza, A., Toubro, S., Stender, S., & Astrup, A. V. (2009) · International Journal of Obesity · doi:10.1038/ijo.2009.27 · Cited by 58 · see citing works
Adverse Effects of Supplemental l -Arginine in Atherosclerosis
Loscalzo, J. (2003) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.0000040860.71626.9d · Cited by 58 · see citing works
Sensitive Amplified Immunoenzymometric Assays (IEMA) for Human Insulin and Intact Proinsulin
Alpha‐Bazin, B., Cox, L., Crowther, N. J., CLARK, P. M. S., & Hales, C. N. (1992) · Clinical Chemistry and Laboratory Medicine (CCLM) · doi:10.1515/cclm.1992.30.1.27 · Cited by 49 · see citing works
Effects of Dietary L-Arginine Intake on Cardiorespiratory and Metabolic Adaptation in Athletes
Bescós, R., González‐Haro, C., Pujol, P., Drobnic, F., Alonso, E., Santolaria, M. L., Ruiz, O. L. C., Estève, M., & Galilea, P. A. (2009) · International Journal of Sport Nutrition and Exercise Metabolism · doi:10.1123/ijsnem.19.4.355 · Cited by 47 · see citing works
Clifton, P. M. (2003) · Current Atherosclerosis Reports · doi:10.1007/s11883-003-0032-z · Cited by 40 · see citing works
Kang, B. P., Bansal, M. P., & Mehta, U. (1998) · PubMed · Cited by 40 · see citing works
Pea fiber lowers fasting and postprandial blood triglyceride concentrations in humans.
Sandström, B., Hansen, L. T., & Sørensen, A. (1994) · PubMed · doi:10.1093/jn/124.12.386 · Cited by 40 · see citing works
Yun, C., Yang, J. S., Kang, S., et al. (2007) · International Immunopharmacology · doi:10.1016/j.intimp.2007.05.002 · Cited by 33 · see citing works
Effects of Arginine Supplementation on Post-Exercise Metabolic Responses
Tsai, P.-H. (2009) · The Chinese Journal of Physiology · doi:10.4077/cjp.2009.amh037 · Cited by 33 · see citing works
Boualga, A., Prost, J., Taleb-Senouci, D., et al. (2009) · European Journal of Nutrition · doi:10.1007/s00394-009-0777-4 · Cited by 30 · see citing works
Jabłecka, A., Chęciński, P., Krauss, H., et al. (2004) · PubMed · Cited by 27 · see citing works
Influence of dietary golden pea proteins versus casein on plasma and hepatic lipids in rats
Lasekan, J. B., Gueth, L., & Khan, S. (1995) · Nutrition Research · doi:10.1016/0271-5317(95)91654-u · Cited by 24 · see citing works
Serum Triglyceride Elevation Following High-Dose Vitamin A Treatment for Pityriasis Rubra Pilaris
Murray, J. C. S. (1983) · Archives of Dermatology · doi:10.1001/archderm.1983.01650320049015 · Cited by 24 · see citing works
Huang, H., Ma, M., & Chen, J. (2008) · American Journal of Physiology-Renal Physiology · doi:10.1152/ajprenal.00405.2007 · Cited by 23 · see citing works
Serum Selenium Levels in Healthy Women in Tabriz, Iran
Rafraf, M., Mahdavi, R., & Rashidi, M. R. (2008) · Food and Nutrition Bulletin · doi:10.1177/156482650802900201 · Cited by 20 · see citing works
Tabatabaei, N., Jamalian, J., Owji, A. A., et al. (2008) · Food and Chemical Toxicology · doi:10.1016/j.fct.2008.08.029 · Cited by 18 · see citing works
Kang, B., Mehta, U., & Bansal, M. P. (1997) · Archives of Physiology and Biochemistry · doi:10.1076/apab.105.6.603.3271 · Cited by 17 · see citing works
Simonet, S., Rupin, A., Badier‐Commander, C., Coumailleau, S., Behr‐Roussel, D., & Verbeuren, T. J. (2004) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2004.03.052 · Cited by 16 · see citing works
Alizadeh, M., Daneghian, S., Ghaffari, A., Ostadrahimi, A., Safaeiyan, A., Estakhri, R., & Gargari, B. P. (2010) · Europe PMC (PubMed Central) · Cited by 15 · see citing works
Self-citationRedlich, C. A., Chung, J. S., Cullen, M. R., et al. (1999) · Atherosclerosis · doi:10.1016/s0021-9150(99)00270-1 · Cited by 14 · see citing works
The Effect of Weight Loss on C-Reactive Protein: A Systematic Review
Selvin, E. (2007) · Archives of Internal Medicine · doi:10.1001/archinte.167.1.31 · Cited by 5 · see citing works
Immunoturbidimetric C-reactive protein kit adapted to the Technicon RA-1000.
Harrison, S. P., & Barlow, I. M. (1988) · Clinical Chemistry · doi:10.1093/clinchem/34.1.172a · Cited by 2 · see citing works
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Regulation of obesity and insulin resistance by nitric oxide
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- Authors
- Alizadeh, M., Safaeiyan, A., Ostadrahimi, A., Estakhri, R., Daneghian, S., Ghaffari, A., & Gargari, B. P.
- Affiliations
- Urmia University of Medical Sciences (IR)
- Tabriz University of Medical Sciences (IR)
- ORCID
- Venue
- Annals of Nutrition and Metabolism
- Publisher
- S. Karger AG
- Published
- 2012
- Volume
- 60
- Issue
- 2
- Pages
- 157-168
- ISSN
- 0250-6807 (ISSN-L)
- 1421-9697
- OpenAlex
- W2083743440
- Cited by
- 76
- References
- 71
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 2 · 2023: 5 · 2022: 7 · 2021: 3 · 2020: 6 · 2019: 8 · 2018: 6 · 2017: 3 · 2016: 9 · 2015: 5 · 2014: 9 · 2013: 6 · 2012: 2
- FWCI
- 6.79
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Annals of Nutrition and Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of Tabriz
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Nutritional Studies and Diet
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes, Cardiovascular Risks, and Lipoproteins
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Keywords
- L-arginine, selenium, hypocaloric diet, Fabaceae, abdominal obesity, cardiovascular risk factors, premenopausal women, insulin resistance, nitric oxide, C-reactive protein, triglycerides, waist circumference, weight loss
- MeSH terms
- Adult, Arginine, Cardiovascular Diseases, Diet, Reducing, Double-Blind Method, Female, Humans, Insulin Resistance, Iran, Fabaceae, Middle Aged, Nitric Oxide, Risk Factors, Seeds, Selenium, Hypertriglyceridemia, Weight Loss, Dietary Supplements, Waist Circumference, Obesity, Abdominal
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