Triglyceride-rich lipoproteins isolated by selected-affinity anti-apolipoprotein B immunosorption from human atherosclerotic plaque
Open accessAbstract
We isolated and characterized immunoreactive apolipoprotein B (apoB)-containing lipoproteins from human atherosclerotic plaque and plasma to determine whether very-low-density lipoprotein (VLDL) can enter and become incorporated into the atherosclerotic lesion and how plaque apoB-containing lipoproteins differ from apoB-containing lipoproteins isolated from plasma. Atherosclerotic plaques were obtained during aortic surgery and processed immediately. Lipoproteins were extracted from minced plaque in a buffered saline solution (extract A). In selected cases a second extraction was done after plaque was incubated with collagenase (extract B). Lipoproteins were then isolated from the extracts by anti-apoB immunosorption and separated into VLDL + intermediate-density lipoprotein (IDL) (d < 1.019 g/mL) and low-density lipoprotein (LDL) (1.019 < d < 1.070 g/mL) fractions by ultracentrifugation. The VLDL + IDL fractions from plaque contained more than one third of the total apoB-associated lipoprotein cholesterol in both extracts A and B. The lipid composition of VLDL + IDL in both extracts was related to that of plasma VLDL + IDL. By electron microscopy mean particle diameters of VLDL + IDL from extracts A and B were 9% and 23%, respectively, greater than VLDL + IDL diameters from plasma. Mean diameters of LDL from extracts A and B were 11% and 31% greater than LDL diameters from plasma. The apoE-apoB ratio of extract A VLDL + IDL was nearly twice that of plasma VLDL + IDL and severalfold higher than that of extract A LDL. Immunoblots of both VLDL + IDL and LDL from extract A demonstrated minimal fragmentation of apoB.(ABSTRACT TRUNCATED AT 250 WORDS)
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Both apoB48- and apoB100-containing remnant particles have been identified in human atherosclerotic lesions.
“Both apoB48- and apoB100-containing remnant particles have been identified in human atherosclerotic lesions.147,148”
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- Authors
- Rapp, J. H., Lespine, A., Hamilton, R. L., Colyvas, N., Chaumeton, A. H., Tweedie-Hardman, J., Kotite, L., Kunitake, S. T., Havel, R. J., & Kane, J. P.
- Affiliations
- University of California, San Francisco (US)
- Venue
- Arteriosclerosis and Thrombosis: A Journal of Vascular Biology
- Published
- 1994-11
- Volume
- 14
- Issue
- 11
- Pages
- 1767-1774
- ISSN
- 1049-8834 (ISSN-L)
- OpenAlex
- W2126273261
- Cited by
- 340
- References
- 40
- Citations by year
- 2025: 5 · 2024: 5 · 2023: 5 · 2022: 7 · 2021: 9 · 2020: 6 · 2019: 4 · 2018: 7 · 2017: 11 · 2016: 6 · 2015: 9 · 2014: 13 · 2013: 13 · 2012: 8
- FWCI
- 15.22
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Arteriosclerosis and Thrombosis A Journal of Vascular Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r29hl041470-05, 5p50hl014237-25)
- National Heart, Lung, and Blood Institute (HL 14237, HL41470)
- Topics
- Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
- Health Sciences › Medicine › Surgery › Lipoproteins and Cardiovascular Health
- Life Sciences › Immunology and Microbiology › Immunology › Atherosclerosis and Cardiovascular Diseases
- Keywords
- triglyceride-rich lipoproteins, apolipoprotein B, atherosclerotic plaque, VLDL, intermediate density lipoprotein, low-density lipoprotein, lipoprotein particle size, atherosclerosis
- MeSH terms
- Aged, Antibodies, Apolipoproteins B, Apolipoproteins E, Arteriosclerosis, Female, Humans, Immunoelectrophoresis, Immunosorbent Techniques, Lipoproteins, Male, Middle Aged, Particle Size, Triglycerides
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