The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Electron Spin Resonance Characterization of Vascular Xanthine and NAD(P)H Oxidase Activity in Patients With Coronary Artery Disease: Relation to Endothelium-Dependent Vasodilation
Abstract
Background— Increased inactivation of nitric oxide by superoxide (O 2 ·− ) contributes to endothelial dysfunction in patients with coronary disease (CAD). We therefore characterized the vascular activities of xanthine oxidase and NAD(P)H oxidase, 2 major O 2 ·− -producing enzyme systems, and their relationship with flow-dependent, endothelium-mediated vasodilation (FDD) in patients with CAD. Methods and Results— Xanthine- and NAD(P)H-mediated O 2 ·− formation was determined in coronary arteries from 10 patients with CAD and 10 controls by using electron spin resonance spectroscopy. Furthermore, activity of endothelium-bound xanthine oxidase in vivo and FDD of the radial artery were determined in 21 patients with CAD and 10 controls. FDD was measured before and after infusion of the antioxidant vitamin C (25 mg/min i.a.) to determine the portion of FDD inhibited by radicals. In coronary arteries from patients with CAD, xanthine- and NAD(P)H-mediated O 2 ·− formation was increased compared with controls (xanthine: 12±2 versus 7±1 nmol O 2 ·− /μg protein; NADH: 11±1 versus 7±1 nmol O 2 ·− /μg protein; and NADPH: 12±2 versus 9±1 nmol O 2 ·− /μg protein; each P <0.05). Endothelium-bound xanthine oxidase activity was increased by >200% in patients with CAD (25±4 versus 9±1 nmol O 2 ·− /μL plasma per min; P <0.05) and correlated inversely with FDD ( r =−0.55; P <0.05) and positively with the effect of vitamin C on FDD ( r =0.54; P <0.05). Conclusions— The present study represents the first electron spin resonance measurements of xanthine and NAD(P)H oxidase activity in human coronary arteries and supports the concept that increased activities of both enzymes contribute to increased vascular oxidant stress in patients with CAD. Furthermore, the present study suggests that increased xanthine oxidase activity contributes to endothelial dysfunction in patients with CAD and may thereby promote the atherosclerotic process.
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Cites
Hypercholesterolemia increases endothelial superoxide anion production.
Ohara, Y., Peterson, T. E., & Harrison, D. G. (1993) · Journal of Clinical Investigation · doi:10.1172/jci116491 · Cited by 1,765
Self-citationAscorbic Acid Reverses Endothelial Vasomotor Dysfunction in Patients With Coronary Artery Disease
Levine, G. N., Frei, B., Koulouris, S. N., Gerhard, M. D., Keaney, J. F., & Vita, J. A. (1996) · Circulation · doi:10.1161/01.cir.93.6.1107 · Cited by 582
Gokce, N., Keaney, J. F., Frei, B., Holbrook, M., Olesiak, M., Zachariah, B. J., Leeuwenburgh, C., Heinecke, J. W., & Vita, J. A. (1999) · Circulation · doi:10.1161/01.cir.99.25.3234 · Cited by 387
White, C. R., Darley-Usmar, V, Berrington, W. R., McAdams, M., Gore, J. Z., Thompson, J. A., Parks, D. A., Tarpey, M. M., & Freeman, B. A. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.16.8745 · Cited by 367
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Cited by
The NOX Family of ROS-Generating NADPH Oxidases: Physiology and Pathophysiology
Bedard, K., & Krause, K.-H. (2007) · Physiological Reviews · doi:10.1152/physrev.00044.2005 · Cited by 6,989
Endothelial Function and Dysfunction: Testing and Clinical Relevance
Deanfield, J. E., Halcox, J. P., & Rabelink, T. J. (2007) · Circulation · doi:10.1161/circulationaha.106.652859 · Cited by 2,710
Oxidative Stress and Vascular Disease
Madamanchi, N. R., Vendrov, A., & Runge, M. S. (2005) · Arteriosclerosis, Thrombosis, and Vascular Biology · doi:10.1161/01.atv.0000150649.39934.13 · Cited by 1,618
Endothelial Function: A Critical Determinant in Atherosclerosis?
Landmesser, U., Hornig, B., & Drexler, H. (2004) · Circulation · doi:10.1161/01.cir.0000129501.88485.1f · Cited by 851
Self-citationCross talk between mitochondria and NADPH oxidases
Dikalov, S. (2011) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2011.06.033 · Cited by 783
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Metadata
- Authors
- Spiekermann, S., Landmesser, U., Dikalov, S., Bredt, M., Gamez, G., Tatge, H., Reepschläger, N., Hornig, B., Drexler, H., & Harrison, D. G.
- Venue
- Circulation
- Published
- 2003-03-18
- Volume
- 107
- Issue
- 10
- Pages
- 1383-1389
- ISSN
- 0009-7322 (ISSN-L)
- 1524-4539
- OpenAlex
- W2128651724
- Cited by
- 298
- References
- 5
- FWCI
- 12.30
- Type
- journal article
- Language
- en
- Open access
- closed
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- Spiekermann, S., Landmesser, U., Dikalov, S., Bredt, M., Gamez, G., Tatge, H., Reepschläger, N., Hornig, B., Drexler, H., & Harrison, D. G. (2003). Electron Spin Resonance Characterization of Vascular Xanthine and NAD(P)H Oxidase Activity in Patients With Coronary Artery Disease: Relation to Endothelium-Dependent Vasodilation. Circulation. https://doi.org/10.1161/01.cir.0000056762.69302.46
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