Lung microbiota and bacterial abundance in patients with bronchiectasis when clinically stable and during exacerbation
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RATIONALE: Characterization of bacterial populations in infectious respiratory diseases will provide improved understanding of the relationship between the lung microbiota, disease pathogenesis, and treatment outcomes. OBJECTIVES: To comprehensively define lung microbiota composition during stable disease and exacerbation in patients with bronchiectasis. METHODS: Sputum was collected from patients when clinically stable and before and after completion of antibiotic treatment of exacerbations. Bacterial abundance and community composition were analyzed using anaerobic culture and 16S rDNA pyrosequencing. MEASUREMENTS AND MAIN RESULTS: In clinically stable patients, aerobic and anaerobic bacteria were detected in 40 of 40 (100%) and 33 of 40 (83%) sputum samples, respectively. The dominant organisms cultured were Pseudomonas aeruginosa (n = 10 patients), Haemophilus influenzae (n = 12), Prevotella (n = 18), and Veillonella (n = 13). Pyrosequencing generated more than 150,000 sequences, representing 113 distinct microbial taxa; the majority of observed community richness resulted from taxa present in low abundance with similar patterns of phyla distribution in clinically stable patients and patients at the onset of exacerbation. After treatment of exacerbation, there was no change in total (P = 0.925), aerobic (P = 0.917), or anaerobic (P = 0.683) load and only a limited shift in community composition. Agreement for detection of bacteria by culture and pyrosequencing was good for aerobic bacteria such as P. aeruginosa (κ = 0.84) but poorer for other genera including anaerobes. Lack of agreement was largely due to bacteria being detected by pyrosequencing but not by culture. CONCLUSIONS: A complex microbiota is present in the lungs of patients with bronchiectasis and remains stable through treatment of exacerbations, suggesting that changes in microbiota composition do not account for exacerbations.
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Culture-independent studies comparing bacterial communities at baseline and during exacerbations in the airways of patients with COPD, cystic fibrosis, or bronchiectasis consistently report no increase in bacterial burden and no decrease in community diversity during exacerbations.
“With remarkable consistency, all studies report no increase in bacterial burden and no decrease in community diversity during exacerbations.”
From The microbiome and critical illness (2016) · cites this paper as [139]
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- [17]Einarsson GG, Klem E, Fodor AA, Wei L, Drain M, Wolfgang MC, Elborn JS, Tunney MM. Microbial community composition in the lungs of patients with cystic fibrosis and non-CF bronchiectasis [abstract] Thorax. 2011;66:A48. doi: 10.1136/thx.2010.137281. [DOI] [PMC free article] [PubMed] [Google Scholar] · DOI
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- Authors
- Tunney, M. M., Einarsson, G. G., Wei, L., Drain, M., Klem, E. R., Cardwell, C., Ennis, M., Boucher, R. C., Wolfgang, M. C., & Elborn, J. S.
- Affiliations
- Cystic Fibrosis and Airways Microbiology Research Group
- Centre for Infection and Immunity, School of Medicine, Dentistry, and Biomedical Sciences, Queen’s University Belfast, Belfast, United Kingdom
- University of North Carolina at Chapel Hill (US)
- Queen's University Belfast (GB)
- Corresponding author
- Tunney, Michael M.
- ORCID
- Venue
- American Journal of Respiratory and Critical Care Medicine
- Publisher
- Oxford University Press (OUP)
- Published
- 2013-05
- Volume
- 187
- Issue
- 10
- Pages
- 1118-1126
- ISSN
- 1073-449X (ISSN-L)
- 1535-4970
- PMC
- PMC3734618
- OpenAlex
- W2116345416
- Cited by
- 316
- References
- 62
- Citations by year
- 2026: 9 · 2025: 11 · 2024: 25 · 2023: 21 · 2022: 20 · 2021: 22 · 2020: 18 · 2019: 24 · 2018: 37 · 2017: 35 · 2016: 31 · 2015: 26 · 2014: 31 · 2013: 6
- FWCI
- 25.52
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- American Journal of Respiratory and Critical Care Medicine (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Research Portal (Queen's University Belfast) (submittedVersion)
- Funders
- Public Health Agency (STL/3713/07, CDV/3650/07)
- National Heart, Lung, and Blood Institute (R01HL092964, HL092964)
- Topics
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Cystic Fibrosis Research Advances
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Keywords
- bronchiectasis, lung microbiome, bacterial abundance, exacerbations, sputum microbiome, Pseudomonas aeruginosa, Haemophilus influenzae, Prevotella, Veillonella, anaerobic bacteria, bacterial community composition, antibiotic therapy
- MeSH terms
- Aged, Anti-Bacterial Agents, Bronchiectasis, Cross-Sectional Studies, Female, Humans, Longitudinal Studies, Lung, Male, Sputum, Cohort Studies, Metagenome
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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