The DOI matches its Crossref record; 50 of the 50 references it cites were found in a registry or an index.
Peroxynitrite inhibits leukocyte-endothelial cell interactions and protects against ischemia-reperfusion injury in rats.
Open accessAbstract
Peroxynitrite (ONOO-) anion, formed by the interaction of superoxide with nitric oxide (NO), has previously been implicated as a cytotoxic agent. However, the effects of this free radical species on neutrophil (PMN)-endothelial cell interactions is largely unknown. We investigated the direct actions of ONOO- on PMN adhesion to endothelial cells in vitro and in vivo, as well as the effects of ONOO- on PMN-mediated myocardial ischemia-reperfusion injury. In vitro, peroxynitrite (100-1,000 nM) inhibited the adhesion of rat PMNs to the endothelium of isolated thrombin- or H2O2-stimulated rat mesenteric artery (P < 0.01 vs. thrombin or H2O2 alone). In vivo, in the rat mesentery, thrombin (0.5 U/ml) or N(G)-nitro-L-arginine-methyl ester (50 microM) significantly increased venular leukocyte rolling and adherence, which were also significantly (P < 0.01) attenuated by ONOO (800 nM) accompanied by reduced P-selectin expression on the endothelial cell surface. Isolated perfused rat hearts were subjected to global ischemia and reperfusion with rat PMNs (10(8) cells), which resulted in profound cardiac depression (i.e., a marked reduction in left ventricular developed pressure and maximal rate of development of left ventricular pressure). Infusion of ONOO- reversed the myocardial contractile dysfunction of ischemic-reperfused rat hearts to near baseline levels, and markedly attenuated the accumulation of PMNs in the postischemic heart. The present study provides strong evidence that nanomolar concentrations of ONOO- both inhibit leukocyte-endothelial cell interactions and exert cytoprotective effects in myocardial ischemia-reperfusion injury. Furthermore, our results suggest that the inhibition of P-selectin expression by peroxynitrite is a key mechanism of the modulatory actions of ONOO- on leukocyte-endothelial cell interactions.
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Cites
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The chemistry of peroxynitrite: A product from the reaction of nitric oxide with superoxide
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“The metabolism of nitric oxide is complex, and NO has been shown to interact with a number of oxygen-derived free radicals including superoxide to form ONOO(2), which can then be protonated to form peroxynitrous acid, a relatively stable oxidant species (18, 19).”
BackgroundRadomski, M. W., Palmer, R. M. J., & Moncada, S. (1987) · British Journal of Pharmacology · doi:10.1111/j.1476-5381.1987.tb11367.x · Cited by 1,164
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[23] Oxidative chemistry of peroxynitrite
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Cited by
Nitric Oxide and Peroxynitrite in Health and Disease
Pacher, P., Beckman, J. S., & Liaudet, L. (2007) · Physiological Reviews · doi:10.1152/physrev.00029.2006 · Cited by 6,390
Biological aspects of reactive nitrogen species
Patel, R. P., McAndrew, J., Sellak, H., White, C. R., Jo, H., Freeman, B. A., & Darley-Usmar, V. M. (1999) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/s0005-2728(99)00028-6 · Cited by 498
Nitric oxide in myocardial ischemia/reperfusion injury
Schulz, R. (2004) · Cardiovascular Research · doi:10.1016/j.cardiores.2003.09.019 · Cited by 462
Nitric oxide, a protective molecule in the cardiovascular system
Lei, J., Vodovotz, Y., Tzeng, E., & Billiar, T. R. (2013) · Nitric Oxide · doi:10.1016/j.niox.2013.09.004 · Cited by 184
Insulin says NO to cardiovascular disease
Yu, Q., Gao, F., & Ma, X. L. (2011) · Cardiovascular Research · doi:10.1093/cvr/cvq349 · Cited by 172
Metadata
- Authors
- Lefer, D. J., Scalia, R., Campbell, B., Nossuli, T., Hayward, R., Salamon, M., Grayson, J., & Lefer, A. M.
- Venue
- Journal of Clinical Investigation
- Published
- 1997-02-15
- Volume
- 99
- Issue
- 4
- Pages
- 684-691
- ISSN
- 0021-9738 (ISSN-L)
- OpenAlex
- W2079043570
- Cited by
- 263
- References
- 50
- FWCI
- 16.84
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Lefer, D. J., Scalia, R., Campbell, B., Nossuli, T., Hayward, R., Salamon, M., Grayson, J., & Lefer, A. M. (1997). Peroxynitrite inhibits leukocyte-endothelial cell interactions and protects against ischemia-reperfusion injury in rats. Journal of Clinical Investigation. https://doi.org/10.1172/jci119212
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
