The DOI matches its Crossref record; 47 of the 47 references it cites were found in a registry or an index.
CCR2 modulates inflammatory and metabolic effects of high-fat feeding
Open accessAbstract
The C-C motif chemokine receptor-2 (CCR2) regulates monocyte and macrophage recruitment and is necessary for macrophage-dependent inflammatory responses and the development of atherosclerosis.Although adipose tissue expression and circulating concentrations of CCL2 (also known as MCP1), a high-affinity ligand for CCR2, are elevated in obesity, the role of CCR2 in metabolic disorders, including insulin resistance, hepatic steatosis, and inflammation associated with obesity, has not been studied.To determine what role CCR2 plays in the development of metabolic phenotypes, we studied the effects of Ccr2 genotype on the development of obesity and its associated phenotypes.Genetic deficiency in Ccr2 reduced food intake and attenuated the development of obesity in mice fed a high-fat diet.In obese mice matched for adiposity, Ccr2 deficiency reduced macrophage content and the inflammatory profile of adipose tissue, increased adiponectin expression, ameliorated hepatic steatosis, and improved systemic glucose homeostasis and insulin sensitivity.In mice with established obesity, short-term treatment with a pharmacological antagonist of CCR2 lowered macrophage content of adipose tissue and improved insulin sensitivity without significantly altering body mass or improving hepatic steatosis.These data suggest that CCR2 influences the development of obesity and associated adipose tissue inflammation and systemic insulin resistance and plays a role in the maintenance of adipose tissue macrophages and insulin resistance once obesity and its metabolic consequences are established.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“Hypertrophy leads to multiple fold increase in infiltration of macrophages in WAT (55, 56), which promotes a pro-inflammatory and insulin resistant environment.”
From Metabolic impact of endogenously produced estrogens by adipose tissue in females and males across the lifespan (2025) · cites this paper as [55]
Experimental — accuracy not yet measuredIn the citing paper
“Adipose tissue macrophages (ATM) have been shown to comprise a significant proportion of the cellular component of adipose tissue in both lean and obese states [60–63].”
From Macrophage polarization: an opportunity for improved outcomes in biomaterials and regenerative medicine (2012) · cites this paper as [62]
In the citing paper
“In normal rodents and humans, ATMs make up as much as ten percent of the cellular constituents of the tissue [62]. In comparison, it has been shown that in obese subjects that number rises to as much as 40% [62].”
From Macrophage polarization: an opportunity for improved outcomes in biomaterials and regenerative medicine (2012) · cites this paper as [62]
In the citing paper
“Although a marginal relationship between CRP concentration and the Mediterranean diet was observed, this association was no longer significant when other cardiovascular risk factors were considered in the models.”
From Dietary factors and low-grade inflammation in relation to overweight and obesity (2011) · cites this paper as [282]
In the citing paper
“This association was independent of several known cardiovascular risk factors, and persisted when twins within pairs were compared, suggesting that the results were not confounded by shared environmental and genetic factors.”
From Dietary factors and low-grade inflammation in relation to overweight and obesity (2011) · cites this paper as [282]
In the citing paper
“Observational studies have examined the association of the Mediterranean diet with inflammatory markers in healthy persons (282,283,285) , and they generally report inverse correlations.”
From Dietary factors and low-grade inflammation in relation to overweight and obesity (2011) · cites this paper as [282]
In the citing paper
“In a recent study (282) investigating psychological, behavioural and biological risk factors for subclinical CVD in 345 middleaged male twins, an inverse association between adherence to the Mediterranean diet and inflammation, as measured by plasma IL-6 concentration, was noted.”
From Dietary factors and low-grade inflammation in relation to overweight and obesity (2011) · cites this paper as [282]
In the citing paper
“Ccl2 and/or Ccr2 expression is increased in obese adipose tissue (119-122)”
From Insulin sensitivity: Modulation by nutrients and inflammation (2008) · cites this paper as [120]
In the citing paper
“In addition, KO of Ccl2 or Ccr2 or neutralization of CCL2 leads to decreased macrophage recruitment to adipose tissue and protection from obesity-induced insulin resistance (120, 121).”
From Insulin sensitivity: Modulation by nutrients and inflammation (2008) · cites this paper as [120]
In the citing paper
“Other factors produced by inflammatory cells have since been shown to inhibit insulin signaling in the adipocyte including IL-6, IFN-γ and CCL2 (72,73).”
From The ominous triad of adipose tissue dysfunction: inflammation, fibrosis, and impaired angiogenesis. (2017) · cites this paper as [73]
In the citing paper
“There is considerable evidence for the pathophysiological role of macrophage-and/or hypertrophic adipocytederived chemotactic MCP-1/CCR2 pathways in the regulation of monocyte accumulation in obese AT (16,17).”
From Adipose tissue remodeling and obesity (2011) · cites this paper as [17]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Vandesompele, J., De Preter, K., Pattyn, F., Poppe, B., Van Roy, N., De Paepe, A., & Speleman, F. (2002) · Genome Biology · doi:10.1186/gb-2002-3-7-research0034 · Cited by 20,038
Obesity is associated with macrophage accumulation in adipose tissue
Weisberg, S. P., McCann, D., Desai, M., Rosenbaum, M., Leibel, R. L., & Ferrante, A. W. (2003) · Journal of Clinical Investigation · doi:10.1172/jci200319246 · Cited by 10,041
Self-citationAdipose Expression of Tumor Necrosis Factor-α: Direct Role in Obesity-Linked Insulin Resistance
Hotamisligil, G. S., Shargill, N. S., & Spiegelman, B. M. (1993) · Science · doi:10.1126/science.7678183 · Cited by 7,518
Xu, H., Barnes, G. T., Yang, Q., Tan, G., Yang, D., Chou, C. J., Sole, J., Nichols, A., Ross, J. S., Tartaglia, L. A., et al. (2003) · Journal of Clinical Investigation · doi:10.1172/jci200319451 · Cited by 6,760
Inflammation, stress, and diabetes
Wellen, K. E., & Hotamisligil, G. S. (2005) · Journal of Clinical Investigation · doi:10.1172/jci25102 · Cited by 4,143
A central role for JNK in obesity and insulin resistance
Hirosumi, J., Tuncman, G., Chang, L., Görgün, C. Z., Uysal, K. T., Maeda, K., Karin, M., & Hotamisligil, G. S. (2002) · Nature · doi:10.1038/nature01137 · Cited by 3,359
Local and systemic insulin resistance resulting from hepatic activation of IKK-β and NF-κB
Cai, D., Yuan, M., Frantz, D. F., Melendez, P. A., Hansen, L., Lee, J., & Shoelson, S. E. (2005) · Nature Medicine · doi:10.1038/nm1166 · Cited by 2,245
Adipose Tissue, Inflammation, and Cardiovascular Disease
Berg, A. H., & Scherer, P. E. (2005) · Circulation Research · doi:10.1161/01.res.0000163635.62927.34 · Cited by 2,113
Boring, L., Gosling, J., Cleary, M., & Charo, I. F. (1998) · Nature · doi:10.1038/29788 · Cited by 1,965
Self-citationYuan, M., Konstantopoulos, N., Lee, J., Hansen, L., Li, Z.-W., Karin, M., & Shoelson, S. E. (2001) · Science · doi:10.1126/science.1061620 · Cited by 1,884
Research works printed but not linked: 4
- 10.1172/JCI200525102 · DOI · Paper page
- Fried, 1998, J. Clin. Endocrinol. Metab., 83, 847
- 10.1172/JCI200421752 · DOI · Paper page
- Dandona, 1998, J. Clin. Endocrinol. Metab., 83, 2907
Cited by
Sorted by citations
Items 1,001–1,002 of 1,002
Immunometabolism in type 2 diabetes mellitus: tissue-specific interactions
Pinheiro-Machado, E., Gurgul-Convey, E., & Marzec, M. (2020) · Archives of Medical Science · doi:10.5114/aoms.2020.92674 · Cited by 19
Guerre-Millo, M. (2006) · Journal de la Société de Biologie · doi:10.1051/jbio:2006005 · Cited by 16
Metadata
- Authors
- Weisberg, S. P., Hunter, D., Huber, R., Lemieux, J., Slaymaker, S., Vaddi, K., Charo, I., Leibel, R. L., & Jr., A. W. F.
- Affiliations
- Columbia University Irving Medical Center (US)
- Incyte (United States) (US)
- Columbia University (US)
- Gladstone Institutes (US)
- Venue
- Journal of Clinical Investigation
- Published
- 2006-01-04
- Volume
- 116
- Issue
- 1
- Pages
- 115-124
- ISSN
- 0021-9738 (ISSN-L)
- PMC
- PMC1307559
- OpenAlex
- W4250725029
- Cited by
- 1,497
- References
- 47
- Citations by year
- 2026: 32 · 2025: 53 · 2024: 42 · 2023: 59 · 2022: 53 · 2021: 71 · 2020: 67 · 2019: 65 · 2018: 74 · 2017: 77 · 2016: 104 · 2015: 76 · 2014: 83 · 2013: 108 · 2012: 93
- FWCI
- 43.36
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- public-domain
- Locations
- Journal of Clinical Investigation (publishedVersion, public-domain)
- PubMed Central (publishedVersion)
- Funders
- National Institutes of Health (DK066525)
- National Institute of Diabetes and Digestive and Kidney Diseases (DK-063608, DK59960, R56DK066525, (DK066525, K08DK059960, P30DK063608, R01DK066525)
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adipokines, Inflammation, and Metabolic Diseases
- Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiovascular Disease and Adiposity
- Keywords
- CCR2, obesity, high-fat diet, adipose tissue inflammation, macrophage recruitment, insulin resistance, hepatic steatosis, glucose homeostasis, adiponectin, CCR2 knockout mice, CCR2 antagonist, metabolic inflammation
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
