The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.
Analysis of the biologic functions of H- and L-ferritins in HeLa cells by transfection with siRNAs and cDNAs: evidence for a proliferative role of L-ferritin
Open accessAbstract
We describe the use of small interfering RNAs (siRNAs) to down-regulate H- and L-ferritin levels in HeLa cells. siRNAs repressed H- and L-ferritin expression to about 20% to 25% of the background level in both stable and transient transfections. HeLa cells transfected with H- and L-ferritin cDNAs were analyzed in parallel to compare the effects of ferritin up- and down-regulation. We found that large modifications of L-ferritin levels did not affect iron availability in HeLa cells but positively affected cell proliferation rate in an iron-independent manner. The transient down-regulation of H-ferritin modified cellular iron availability and resistance to oxidative damage, as expected. In contrast, the stable suppression of H-ferritin in HeLa cell clones transfected with siRNAs did not increase cellular iron availability but made cells less resistant to iron supplementation and chelation. The results indicate that L-ferritin has no direct effects on cellular iron homeostasis in HeLa cells, while it has new, iron-unrelated functions. In addition, they suggest that H-ferritin function is to act as an iron buffer.
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“In addition, HeLa cells that express mutant ferritin L chains exhibited a phenotype of iron loading (61).”
From Intracellular iron transport and storage: from molecular mechanisms to health implications (2008) · cites this paper as [61]
In the citing paper
“Studies with recombinant mutant ferritin L showed that, although it was able to assemble into 24-subunit ferritin shells, the efficiency of assembly and capacity to incorporate iron were both decreased in vitro (61).”
From Intracellular iron transport and storage: from molecular mechanisms to health implications (2008) · cites this paper as [61]
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“Indeed, over-expression or knockdown of ferritin expression in mammalian cells has proven the cytoprotective role of ferritin under proox-idative conditions (59, 60, 78, 159, 227, 243).”
Metadata
- Authors
- Cozzi, A., Corsi, B., Levi, S., Santambrogio, P., Biasiotto, G., & Arosio, P.
- Affiliations
- IRCCS San Raffaele Roma (IT)
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2004-03-15
- Volume
- 103
- Issue
- 6
- Pages
- 2377-2383
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W1519013154
- Cited by
- 132
- References
- 43
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 3 · 2023: 7 · 2022: 3 · 2021: 2 · 2020: 6 · 2019: 5 · 2018: 1 · 2017: 3 · 2016: 4 · 2015: 7 · 2014: 9 · 2013: 21 · 2012: 4
- FWCI
- 3.07
- Type
- journal article
- Language
- en
- Open access
- Licence
- other-oa
- Locations
- Blood (publishedVersion)
- PubMed (publishedVersion)
- INFM-OAR (INFN Catania) (submittedVersion, other-oa)
- Topics
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Ferroptosis and cancer prognosis
- Life Sciences › Neuroscience › Neurology › Neurological diseases and metabolism
- Keywords
- H-ferritin, L-ferritin, HeLa cells, cell proliferation, iron homeostasis, iron availability, iron supplementation, chelation, siRNA knockdown
- MeSH terms
- Quaternary Ammonium Compounds, Buffers, Cell Division, Ferric Compounds, Ferritins, HeLa Cells, Humans, Iron, Transfection, Down-Regulation, Up-Regulation, Gene Expression, DNA, Complementary, Oxidative Stress, RNA, Small Interfering
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