The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.
Analysis of the biologic functions of H- and L-ferritins in HeLa cells by transfection with siRNAs and cDNAs: evidence for a proliferative role of L-ferritin
Open accessAbstract
We describe the use of small interfering RNAs (siRNAs) to down-regulate H- and L-ferritin levels in HeLa cells. siRNAs repressed H- and L-ferritin expression to about 20% to 25% of the background level in both stable and transient transfections. HeLa cells transfected with H- and L-ferritin cDNAs were analyzed in parallel to compare the effects of ferritin up- and down-regulation. We found that large modifications of L-ferritin levels did not affect iron availability in HeLa cells but positively affected cell proliferation rate in an iron-independent manner. The transient down-regulation of H-ferritin modified cellular iron availability and resistance to oxidative damage, as expected. In contrast, the stable suppression of H-ferritin in HeLa cell clones transfected with siRNAs did not increase cellular iron availability but made cells less resistant to iron supplementation and chelation. The results indicate that L-ferritin has no direct effects on cellular iron homeostasis in HeLa cells, while it has new, iron-unrelated functions. In addition, they suggest that H-ferritin function is to act as an iron buffer.
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In the citing paper
“In addition, HeLa cells that express mutant ferritin L chains exhibited a phenotype of iron loading (61).”
From Intracellular iron transport and storage: from molecular mechanisms to health implications (2008) · cites this paper as [61]
In the citing paper
“Studies with recombinant mutant ferritin L showed that, although it was able to assemble into 24-subunit ferritin shells, the efficiency of assembly and capacity to incorporate iron were both decreased in vitro (61).”
From Intracellular iron transport and storage: from molecular mechanisms to health implications (2008) · cites this paper as [61]
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Cites
A System for Stable Expression of Short Interfering RNAs in Mammalian Cells
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The ferritins: molecular properties, iron storage function and cellular regulation
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Self-citationHentze, M. W., & Kühn, L. C. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.16.8175 · Cited by 1,292
Killing the messenger: short RNAs that silence gene expression
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Mineralization in ferritin: an efficient means of iron storage
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Solving the structure of human H ferritin by genetically engineering intermolecular crystal contacts
Lawson, D. M., Artymiuk, P. J., Yewdall, S. J., Smith, J. M. A., Livingstone, J. C., Treffry, A., Luzzago, A., Levi, S., Arosio, P., Cesareni, G., et al. (1991) · Nature · doi:10.1038/349541a0 · Cited by 777
Self-citationArosio, P., & Levi, S. (2002) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(02)00842-0 · Cited by 553
Self-citationCurtis, A. R. J., Fey, C., Morris, C. M., Bindoff, L. A., Ince, P. G., Chinnery, P. F., Coulthard, A., Jackson, M. J., Jackson, A. P., McHale, D. P., et al. (2001) · Nature Genetics · doi:10.1038/ng571 · Cited by 537
Ferritin and the response to oxidative stress
ORINO, K., LEHMAN, L., TSUJI, Y., AYAKI, H., TORTI, S. V., & TORTI, F. M. (2001) · Biochemical Journal · doi:10.1042/bj3570241 · Cited by 535
A human mitochondrial ferritin encoded by an intronless gene
Levi, S., Corsi, B., Bosisio, M., Invernizzi, R., Volz, A., Sanford, D., Arosio, P., & Drysdale, J. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.c100141200 · Cited by 400
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Iron and cancer: more ore to be mined
Torti, S. V., & Torti, F. M. (2013) · Nature Reviews Cancer · doi:10.1038/nrc3495 · Cited by 1,621
Ferritins: A family of molecules for iron storage, antioxidation and more
Arosio, P., Ingrassia, R., & Cavadini, P. (2009) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2008.09.004 · Cited by 886
Self-citationPham, C. G., Bubici, C., Zazzeroni, F., Papa, S., Jones, J., Alvarez, K., Jayawardena, S., De Smaele, E., Cong, R., Beaumont, C., et al. (2004) · Cell · doi:10.1016/j.cell.2004.10.017 · Cited by 663
Mechanisms of mammalian iron homeostasis
Pantopoulos, K., Porwal, S. K., Tartakoff, A., & Devireddy, L. (2012) · Biochemistry · doi:10.1021/bi300752r · Cited by 598
Intracellular iron transport and storage: from molecular mechanisms to health implications
Mackenzie, E. L., Iwasaki, K., & Tsuji, Y. (2008) · Antioxidants & Redox Signaling · doi:10.1089/ars.2007.1893 · Cited by 526
“Indeed, over-expression or knockdown of ferritin expression in mammalian cells has proven the cytoprotective role of ferritin under proox-idative conditions (59, 60, 78, 159, 227, 243).”
Metadata
- Authors
- Cozzi, A., Corsi, B., Levi, S., Santambrogio, P., Biasiotto, G., & Arosio, P.
- Affiliations
- IRCCS San Raffaele Roma (IT)
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2004-03-15
- Volume
- 103
- Issue
- 6
- Pages
- 2377-2383
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W1519013154
- Cited by
- 132
- References
- 43
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 3 · 2023: 7 · 2022: 3 · 2021: 2 · 2020: 6 · 2019: 5 · 2018: 1 · 2017: 3 · 2016: 4 · 2015: 7 · 2014: 9 · 2013: 21 · 2012: 4
- FWCI
- 3.07
- Type
- journal article
- Language
- en
- Open access
- Licence
- other-oa
- Locations
- Blood (publishedVersion)
- PubMed (publishedVersion)
- INFM-OAR (INFN Catania) (submittedVersion, other-oa)
- Topics
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Ferroptosis and cancer prognosis
- Life Sciences › Neuroscience › Neurology › Neurological diseases and metabolism
- Keywords
- H-ferritin, L-ferritin, HeLa cells, cell proliferation, iron homeostasis, iron availability, iron supplementation, chelation, siRNA knockdown
- MeSH terms
- Quaternary Ammonium Compounds, Buffers, Cell Division, Ferric Compounds, Ferritins, HeLa Cells, Humans, Iron, Transfection, Down-Regulation, Up-Regulation, Gene Expression, DNA, Complementary, Oxidative Stress, RNA, Small Interfering
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