Regulation of lipid metabolism-related gene expression in whole blood cells of normo- and dyslipidemic men after fish oil supplementation
Open accessAbstract
Background: Beneficial effects of omega-3 polyunsaturated fatty acids (n-3 PUFAs) on the lipid levels of dyslipidemic subjects are widely described in the literature. However, the underlying molecular mechanisms are largely unknown. The aim of this study was to investigate the effects of n-3 PUFAs on the expression of lipid metabolism-related genes in normo- and dyslipidemic men to unveil potential genes and pathways affecting lipid metabolism. Methods: Ten normo- and ten dyslipidemic men were supplemented for twelve weeks with six fish oil capsules per day, providing 1.14 g docosahexaenoic acid and 1.56 g eicosapentaenoic acid. The gene expression levels were determined by whole genome microarray analysis and quantitative real-time polymerase chain reaction. Results: Several transcription factors (peroxisome proliferator-activated receptor α (PPARα), retinoid X receptor (RXR) α, RXRγ, hepatic nuclear factor (HNF) 6, and HNF1ß) as well as other genes related to triacylglycerol (TG) synthesis or high-density lipoprotein (HDL-C) and cholesterol metabolism (phospholipids transfer protein, ATP-binding cassette sub-family G member 5, 2-acylglycerol O-acyltransferase (MOGAT) 3, MOGAT2, diacylglycerol O-acyltransferase 1, sterol O-acyltransferase 1, apolipoprotein CII, and low-density lipoprotein receptor) were regulated after n-3 PUFA supplementation, especially in dyslipidemic men. Conclusion: Gene expression analyses revealed several possible molecular pathways by which n-3 PUFAs lower the TG level and increase the HDL-C and low-density lipoprotein level, whereupon the regulation of PPARα appear to play a central role. Trial registration: ClinicalTrials.gov (ID: NCT01089231)
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Expression of LDL receptor mRNA in peripheral blood was not influenced by OM3FA treatment in normolipidemic individuals, but decreased in hyperlipidemic individuals.
“Expression of LDL receptor mRNA in peripheral blood, used as a surrogate marker for expression in the liver, was not influenced by OM3FA treatment in normolipidemic individuals [51, 62], but decreased in hyperlipidemic individuals [62, 63].”
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- Authors
- Schmidt, S., Willers, J., Stahl, F., Mutz, K.-O., Scheper, T., Hahn, A., & Schuchardt, J. P.
- Affiliations
- Leibniz University Hannover (DE)
- Corresponding author
- Schuchardt, Jan Philipp
- Venue
- Lipids in Health and Disease
- Published
- 2012-12
- Volume
- 11
- Issue
- 1
- Pages
- 172-172
- ISSN
- 1476-511X (ISSN-L)
- PMC
- PMC3543286
- OpenAlex
- W2119155873
- Cited by
- 31
- References
- 84
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 3 · 2022: 1 · 2021: 4 · 2020: 3 · 2019: 1 · 2018: 2 · 2017: 1 · 2016: 1 · 2015: 5 · 2014: 6 · 2013: 1
- FWCI
- 3.58
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
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- cc-by
- Locations
- Lipids in Health and Disease (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
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- Institutional Repository of Leibniz Universität Hannover (Leibniz Universität Hannover) (submittedVersion, cc-by-nc-nd)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Leibniz Universität Hannover (cc-by)
- Funders
- Bundesministerium für Bildung und Forschung
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
- Keywords
- omega-3 polyunsaturated fatty acids, fish oil supplementation, dyslipidemia, lipid metabolism, gene expression, PPARα, retinoid X receptor, transcription factors, triglyceride synthesis, HDL cholesterol, LDL cholesterol, apolipoprotein C-II, LDL receptor, diacylglycerol acyltransferase, whole blood cells
- MeSH terms
- Adult, Fish Oils, Humans, Lipids, Cholesterol, HDL, Lipoproteins, LDL, Male, Middle Aged, Triglycerides, Fatty Acids, Omega-3, Gene Expression, Genome, Human, Dietary Supplements, Microarray Analysis, PPAR alpha, Dyslipidemias, Lipid Metabolism
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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