Cited by 31Fatty Acid Research and HealthLipid metabolism and biosynthesisLipid metabolism and disorders

Regulation of lipid metabolism-related gene expression in whole blood cells of normo- and dyslipidemic men after fish oil supplementation

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Schmidt, Simone, Willers, Janina, Stahl, Frank, Mutz, Kai-Oliver, Scheper, Thomas, Hahn, Andreas, Schuchardt, Jan Philipp·2012·Lipids in Health and Disease

Abstract

Background: Beneficial effects of omega-3 polyunsaturated fatty acids (n-3 PUFAs) on the lipid levels of dyslipidemic subjects are widely described in the literature. However, the underlying molecular mechanisms are largely unknown. The aim of this study was to investigate the effects of n-3 PUFAs on the expression of lipid metabolism-related genes in normo- and dyslipidemic men to unveil potential genes and pathways affecting lipid metabolism. Methods: Ten normo- and ten dyslipidemic men were supplemented for twelve weeks with six fish oil capsules per day, providing 1.14 g docosahexaenoic acid and 1.56 g eicosapentaenoic acid. The gene expression levels were determined by whole genome microarray analysis and quantitative real-time polymerase chain reaction. Results: Several transcription factors (peroxisome proliferator-activated receptor α (PPARα), retinoid X receptor (RXR) α, RXRγ, hepatic nuclear factor (HNF) 6, and HNF1ß) as well as other genes related to triacylglycerol (TG) synthesis or high-density lipoprotein (HDL-C) and cholesterol metabolism (phospholipids transfer protein, ATP-binding cassette sub-family G member 5, 2-acylglycerol O-acyltransferase (MOGAT) 3, MOGAT2, diacylglycerol O-acyltransferase 1, sterol O-acyltransferase 1, apolipoprotein CII, and low-density lipoprotein receptor) were regulated after n-3 PUFA supplementation, especially in dyslipidemic men. Conclusion: Gene expression analyses revealed several possible molecular pathways by which n-3 PUFAs lower the TG level and increase the HDL-C and low-density lipoprotein level, whereupon the regulation of PPARα appear to play a central role. Trial registration: ClinicalTrials.gov (ID: NCT01089231)

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Authors
Schmidt, S., Willers, J., Stahl, F., Mutz, K.-O., Scheper, T., Hahn, A., & Schuchardt, J. P.
Affiliations
Leibniz University Hannover (DE)
Corresponding author
Schuchardt, Jan Philipp
Venue
Lipids in Health and Disease
Published
2012-12
Volume
11
Issue
1
Pages
172-172
ISSN
1476-511X (ISSN-L)
OpenAlex
W2119155873
Cited by
31
References
84
Citations by year
2026: 1 · 2025: 2 · 2024: 3 · 2022: 1 · 2021: 4 · 2020: 3 · 2019: 1 · 2018: 2 · 2017: 1 · 2016: 1 · 2015: 5 · 2014: 6 · 2013: 1
FWCI
3.58
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Lipids in Health and Disease (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Institutional Repository of Leibniz Universität Hannover (Leibniz Universität Hannover) (submittedVersion, cc-by-nc-nd)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
  • Leibniz Universität Hannover (cc-by)
Funders
Bundesministerium für Bildung und Forschung
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
Keywords
omega-3 polyunsaturated fatty acids, fish oil supplementation, dyslipidemia, lipid metabolism, gene expression, PPARα, retinoid X receptor, transcription factors, triglyceride synthesis, HDL cholesterol, LDL cholesterol, apolipoprotein C-II, LDL receptor, diacylglycerol acyltransferase, whole blood cells
MeSH terms
Adult, Fish Oils, Humans, Lipids, Cholesterol, HDL, Lipoproteins, LDL, Male, Middle Aged, Triglycerides, Fatty Acids, Omega-3, Gene Expression, Genome, Human, Dietary Supplements, Microarray Analysis, PPAR alpha, Dyslipidemias, Lipid Metabolism

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