Plasma specific miRNAs as predictive biomarkers for diagnosis and prognosis of glioma
Open accessAbstract
OBJECTIVE: Glioblastoma multiforme (GBM) is a highly malignant brain tumor with a poor prognosis. MicroRNAs (miRNAs) are a class of small non-coding RNAs, approximately 21-25 nucleotides in length. Recently, some researchers have demonstrated that plasma miRNAs are sensitive and specific biomarkers of various cancers. The primary aim of the study is to investigate whether miRNAs present in the plasma of GBM patients can be used as diagnostic biomarkers and are associated with glioma classification and clinical treatment. MATERIALS AND METHODS: Plasma samples were attained by venipuncture from 50 patients and 10 healthy donors. Plasma levels of miRNAs were determined by real-time quantitative polymerase chain reaction. RESULTS: The plasma levels of miR-21, miR-128 and miR-342-3p were significantly altered in GBM patients compared to normal controls and could discriminate glioma from healthy controls with high specificity and sensitivity. However, these three miRNAs were not significantly changed in patients with other brain tumors such as meningioma or pituitary adenoma. Furthermore, the plasma levels of these three miRNAs in GBM patients treated by operation and chemo-radiation almost revived to normal levels. Finally, we also demonstrated that miR-128 and miR-342-3p were positively correlated with histopathological grades of glioma. CONCLUSIONS: These findings suggest that plasma specific miRNAs have potential use as novel biomarkers of glioma and may be useful in clinical management for glioma patients.
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Serum miR-21 expression levels were significantly higher in pre-operative glioma samples than in postoperative serum of grade 2 (p=0.0079), grade 3 (p=0.0079) and grade 4 (p=0.0096).
“Moreover, the serum expression levels were also significantly higher in pre-operative glioma samples than in postoperative serum of grade 2 (p=0.0079 ), grade 3 (p=0.0079) and grade 4 (p=0.0096)which is consistent with previous findings [26,30] .”
From miR-21 as a diagnostic and prognostic biomarker in glioma: tissue and serum expression analysis in a Pakistani cohort (2026) · cites this paper as [30]
The Results section on treatment changes reports no significant difference in plasma miR-21 between pre- and postoperative GBM samples (P = 0.393), contrary to the claimed significant decrease. Its grade II–IV comparison concerns glioma samples versus healthy controls, not grade-specific pre- and postoperative serum comparisons. (LLM, conf=0.94)
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Experimental — accuracy not yet measuredThe increase in miR-21 levels may be linked to its role in angiogenesis and tumor proliferation, key features of grade 4 tumors.
“This increase in miR-21 levels may be linked to its role in angiogenesis and tumor proliferation, key features of grade 4 tumors [29,30].”
From miR-21 as a diagnostic and prognostic biomarker in glioma: tissue and serum expression analysis in a Pakistani cohort (2026) · cites this paper as [30]
The Results section on histopathological grade (page not provided) reports elevated plasma miR-21 in grade IV glioblastoma compared with controls, supporting the reported increase. The Discussion (page not provided) describes miR-21 as anti-apoptotic, but does not link the increase to angiogenesis or tumor proliferation. The Results also say miR-21 expression was not associated with glioma grade, so the proposed grade-IV-specific explanation is not supported. (LLM, conf=0.84)
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- Authors
- Wang, Q., Li, P., Li, A., Jiang, W., Wang, H., Wang, J., & Xie, K.
- Affiliations
- Tianjin Huanhu Hospital (CN)
- Tianjin Medical University (CN)
- Tianjin Medical University General Hospital (CN)
- Corresponding author
- Wang, Jinhuan
- Xie, Keliang
- Venue
- Journal of Experimental & Clinical Cancer Research
- Published
- 2012-11-22
- Volume
- 31
- Issue
- 1
- Pages
- 97-97
- ISSN
- 0392-9078 (ISSN-L)
- 1756-9966
- PMC
- PMC3554474
- OpenAlex
- W2099467660
- Cited by
- 202
- References
- 2
- Citations by year
- 2026: 6 · 2025: 10 · 2024: 24 · 2023: 11 · 2022: 15 · 2021: 13 · 2020: 19 · 2019: 14 · 2018: 10 · 2017: 17 · 2016: 12 · 2015: 21 · 2014: 19 · 2013: 11
- FWCI
- 5.42
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Journal of Experimental & Clinical Cancer Research (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- National Natural Science Foundation of China (81101409, 2011KZ108)
- Ministry of Health of the People's Republic of China
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Health Sciences › Medicine › Genetics › Glioma Diagnosis and Treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Keywords
- glioblastoma, glioma, plasma microRNA, diagnostic biomarkers, prognostic factors, miR-21, miR-128, miR-342-3p, histopathological grade, treatment response, brain tumors
- MeSH terms
- Adult, Aged, Brain Neoplasms, Female, Glioblastoma, Humans, Male, Middle Aged, Prognosis, Biomarkers, Tumor, Gene Expression Regulation, Neoplastic, Case-Control Studies, MicroRNAs, Neoplasm Grading
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