Cited by 754Barrier Structure and Function StudiesDrug Transport and Resistance MechanismsExtracellular vesicles in disease

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The hCMEC/D3 cell line as a model of the human blood brain barrier

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Weksler, Babette, Romero, Ignacio A, Couraud, Pierre-Olivier·2013·Fluids and Barriers of the CNS

DOI 10.1186/2045-8118-10-16PMC PMC3623852

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Abstract

Since the first attempts in the 1970s to isolate cerebral microvessel endothelial cells (CECs) in order to model the blood–brain barrier (BBB) in vitro , the need for a human BBB model that closely mimics the in vivo phenotype and is reproducible and easy to grow, has been widely recognized by cerebrovascular researchers in both academia and industry. While primary human CECs would ideally be the model of choice, the paucity of available fresh human cerebral tissue makes wide-scale studies impractical. The brain microvascular endothelial cell line hCMEC/D3 represents one such model of the human BBB that can be easily grown and is amenable to cellular and molecular studies on pathological and drug transport mechanisms with relevance to the central nervous system (CNS). Indeed, since the development of this cell line in 2005 over 100 studies on different aspects of cerebral endothelial biology and pharmacology have been published. Here we review the suitability of this cell line as a human BBB model for pathogenic and drug transport studies and we critically consider its advantages and limitations.

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Self-citation

Authors: Weksler, B., Romero, I. A., & Couraud, P.-O.
Affiliations: Inserm (FR)
The Open University (GB)
Centre National de la Recherche Scientifique (FR)
Corresponding author: Couraud, Pierre-Olivier
ORCID: Weksler, Babette
Romero, Ignacio A
Venue: Fluids and Barriers of the CNS
Publisher: Springer Science and Business Media LLC
Published: 2013-03-26
Volume: 10
Issue: 1
Pages: 16-16
ISSN: 2045-8118 (ISSN-L)
DOI: 10.1186/2045-8118-10-16
PMC: PMC3623852
OpenAlex: W2115924220
Cited by: 753
References: 77
Citations by year: 2026: 45 · 2025: 70 · 2024: 76 · 2023: 75 · 2022: 68 · 2021: 83 · 2020: 58 · 2019: 63 · 2018: 36 · 2017: 53 · 2016: 42 · 2015: 36 · 2014: 36 · 2013: 10
FWCI: 23.39
Type: journal article
Language: en
Open access: gold
doi.org
Licence: cc-by
Locations: Fluids and Barriers of the CNS (publishedVersion, cc-by)
Europe PMC (PubMed Central) (publishedVersion, cc-by)
PubMed Central (publishedVersion, cc-by)
PubMed (publishedVersion)
Open Research Online - ORO (The Open University) (acceptedVersion)
HAL (submittedVersion, other-oa)
Topics: Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
Keywords: blood-brain barrier, in vitro BBB model, drug transport across the blood-brain barrier, brain drug delivery, endothelial cell biology

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The hCMEC/D3 cell line as a model of the human blood brain barrier

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