Cited by 754Barrier Structure and Function StudiesDrug Transport and Resistance MechanismsExtracellular vesicles in disease

The DOI matches its Crossref record; 77 of the 77 references it cites were found in a registry or an index.

The hCMEC/D3 cell line as a model of the human blood brain barrier

Open access

Weksler, Babette, Romero, Ignacio A, Couraud, Pierre-Olivier·2013·Fluids and Barriers of the CNS

DOI 10.1186/2045-8118-10-16PMC PMC3623852

Open this paper in CoChat — free account Open PDF

Abstract

Since the first attempts in the 1970s to isolate cerebral microvessel endothelial cells (CECs) in order to model the blood–brain barrier (BBB) in vitro , the need for a human BBB model that closely mimics the in vivo phenotype and is reproducible and easy to grow, has been widely recognized by cerebrovascular researchers in both academia and industry. While primary human CECs would ideally be the model of choice, the paucity of available fresh human cerebral tissue makes wide-scale studies impractical. The brain microvascular endothelial cell line hCMEC/D3 represents one such model of the human BBB that can be easily grown and is amenable to cellular and molecular studies on pathological and drug transport mechanisms with relevance to the central nervous system (CNS). Indeed, since the development of this cell line in 2005 over 100 studies on different aspects of cerebral endothelial biology and pharmacology have been published. Here we review the suitability of this cell line as a human BBB model for pathogenic and drug transport studies and we critically consider its advantages and limitations.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1186/2045-8118-10-16

Cites

77 of 77 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Blood‐brain barrier‐specific properties of a human adult brain endothelial cell line
Weksler, B. B., Subileau, E.-A., Perrière, N., Charneau, P., Holloway, K. E., Leveque, M., Tricoire-Leignel, H., Nicotra, A., Bourdoulous, S., Turowski, P., et al. (2005) · The FASEB Journal · doi:10.1096/fj.04-3458fje · Cited by 1,325
Self-citation
Wnt/β-catenin signaling controls development of the blood–brain barrier
Liebner, S., Corada, M., Bangsow, T., Babbage, J., Taddei, A., Czupalla, C. J., Reis, M., Felici, A., Wolburg, H., Fruttiger, M., et al. (2008) · The Journal of Cell Biology · doi:10.1083/jcb.200806024 · Cited by 860
Derivation of blood-brain barrier endothelial cells from human pluripotent stem cells
Lippmann, E. S., Azarin, S. M., Kay, J. E., Nessler, R. A., Wilson, H. K., Al-Ahmad, A., Palecek, S. P., & Shusta, E. V. (2012) · Nature Biotechnology · doi:10.1038/nbt.2247 · Cited by 800
Electrical resistance across the blood-brain barrier in anaesthetized rats: a developmental study.
Butt, A. M., Jones, H. C., & Abbott, N. J. (1990) · The Journal of Physiology · doi:10.1113/jphysiol.1990.sp018243 · Cited by 775
Tight junctions at the blood brain barrier: physiological architecture and disease-associated dysregulation
Luissint, A.-C., Artus, C., Glacial, F., Ganeshamoorthy, K., & Couraud, P.-O. (2012) · Fluids and Barriers of the CNS · doi:10.1186/2045-8118-9-23 · Cited by 654
Self-citation
ABC transporters, cytochromes P450 and their main transcription factors: expression at the human blood–brain barrier
Dauchy, S., Dutheil, F., Weaver, R. J., Chassoux, F., Daumas‐Duport, C., Couraud, P., Scherrmann, J., de Waziers, I., & Declèves, X. (2008) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2008.05720.x · Cited by 361
Self-citation
Development of a three-dimensional, all-human in vitro model of the blood–brain barrier using mono-, co-, and tri-cultivation Transwell models
Hatherell, K. E., Couraud, P., Romero, I. A., Weksler, B. B., & Pilkington, G. J. (2011) · Journal of Neuroscience Methods · doi:10.1016/j.jneumeth.2011.05.012 · Cited by 358
Self-citation
The human brain endothelial cell line hCMEC/D3 as a human blood‐brain barrier model for drug transport studies
Poller, B., Gutmann, H., Krähenbühl, S., Weksler, B. B., Romero, I. A., Couraud, P., Tuffin, G., Drewe, J., & Huwyler, J. (2008) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2008.05730.x · Cited by 318
Self-citation
Differential effects of hydrocortisone and TNFα on tight junction proteins in an in vitro model of the human blood–brain barrier
Förster, C. Y., Burek, M., Romero, I. A., Weksler, B. B., Couraud, P., & Drenckhahn, D. (2008) · The Journal of Physiology · doi:10.1113/jphysiol.2007.146852 · Cited by 306
Self-citation
Immortalized Human Brain Endothelial Cells and Flow-Based Vascular Modeling: A Marriage of Convenience for Rational Neurovascular Studies
Cucullo, L., Couraud, P., Weksler, B. B., Romero, I. A., Hossain, M. K., Rapp, E., & Janigro, D. (2007) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1038/sj.jcbfm.9600525 · Cited by 266
Self-citation

Show all 77 references

Research works printed but not linked: 1

Lopez-Ramirez MA, Fischer R, Torres-Badillo CC, Davies HA, Logan K, Pfizenmaier K, Male DK, Sharrack B, Romero IA: Role of caspases in cytokine-induced barrier breakdown in human brain endothelial cells. J Immunol. 2012, 189: 3130-3139. 10.4049/jimmunol.1103460. · DOI

Sorted by citations

Items 101–200 of 754

Blood–brain barrier (BBB)-on-a-chip: a promising breakthrough in brain disease research
Peng, B., Hao, S., Tong, Z., Bai, H., Pan, S., Lim, K., Li, L., Voelcker, N. H., & Huang, W. (2022) · Lab on a Chip · doi:10.1039/d2lc00305h · Cited by 61
Evaluation of the adaptogenic potential exerted by ginsenosides Rb1 and Rg1 against oxidative stress-mediated neurotoxicity in an in vitro neuronal model
Fernández‐Moriano, C., González‐Burgos, E., Peinado, I. I., Lozano, R., & Gómez-Serranillos, M. P. (2017) · PLoS ONE · doi:10.1371/journal.pone.0182933 · Cited by 62
Development of a direct contact astrocyte-human cerebral microvessel endothelial cells blood–brain barrier coculture model
Kulczar, C. D., Lubin, K. E., Lefebvre, S., Miller, D. W., & Knipp, G. T. (2017) · Journal of Pharmacy and Pharmacology · doi:10.1111/jphp.12803 · Cited by 62
A host–gut microbial amino acid co-metabolite, p -cresol glucuronide, promotes blood–brain barrier integrity in vivo
Stachulski, A. V., Knausenberger, T. B.-A., Shah, S. N., Hoyles, L., & McArthur, S. (2023) · Tissue Barriers · doi:10.1080/21688370.2022.2073175 · Cited by 61
In vitro screening of nanomedicines through the blood brain barrier: A critical review
Aparicio-Blanco, J., Martín‐Sabroso, C., & Torres‐Suárez, A. I. (2016) · Biomaterials · doi:10.1016/j.biomaterials.2016.06.051 · Cited by 64
The Emerging Role of N-Methyl-D-Aspartate (NMDA) Receptors in the Cardiovascular System: Physiological Implications, Pathological Consequences, and Therapeutic Perspectives
Soda, T., Brunetti, V., Berra‐Romani, R., & Moccia, F. D. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24043914 · Cited by 65
Blood-Brain Barrier Effects of the Fusarium Mycotoxins Deoxynivalenol, 3 Acetyldeoxynivalenol, and Moniliformin and Their Transfer to the Brain
Behrens, M., Hüwel, S., Galla, H., & Humpf, H. (2015) · PLoS ONE · doi:10.1371/journal.pone.0143640 · Cited by 61
In vitro blood–brain barrier models for drug research: state-of-the-art and new perspectives on reconstituting these models on artificial basement membrane platforms
Banerjee, J., Shi, Y., & Azevedo, H. S. (2016) · Drug Discovery Today · doi:10.1016/j.drudis.2016.05.020 · Cited by 64
Carrier-Mediated Cocaine Transport at the Blood-Brain Barrier as a Putative Mechanism in Addiction Liability
Chapy, H., Smirnova, M., André, P., Schlatter, J., Chiadmi, F., Couraud, P., Scherrmann, J., Declèves, X., & Cisternino, S. (2014) · The International Journal of Neuropsychopharmacology · doi:10.1093/ijnp/pyu001 · Cited by 67
Self-citation
Branched BBB-shuttle peptides: chemoselective modification of proteins to enhance blood–brain barrier transport
Díaz‐Perlas, C., Oller‐Salvia, B., Sánchez‐Navarro, M., Teixidò, M., & Giralt, E. (2018) · Chemical Science · doi:10.1039/c8sc02415d · Cited by 59
Nonionotropic Action of Endothelial NMDA Receptors on Blood–Brain Barrier Permeability via Rho/ROCK-Mediated Phosphorylation of Myosin
Mehra, A., Guérit, S., Macrez, R., Gosselet, F., Sevin, E., Lebas, H., Maubert, E., de Vries, H. E., Bardou, I., Vivien, D., et al. (2020) · Journal of Neuroscience · doi:10.1523/jneurosci.0969-19.2019 · Cited by 59
Mechanism of drug extrusion by brain endothelial cells via lysosomal drug trapping and disposal by neutrophils
Noack, A., Gericke, B., von Köckritz‐Blickwede, M., Menze, A., Noack, S., Gerhauser, I., Osten, F., Naim, H. Y., & Löscher, W. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1719642115 · Cited by 59
Enhanced dissolution, permeation and oral bioavailability of aripiprazole mixed micelles: In vitro and in vivo evaluation
Piazzini, V., Landucci, E., Urru, M., Chiarugi, A., Pellegrini-Giampietro, D. E., Bilia, A. R., & Bergonzi, M. C. (2020) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2020.119361 · Cited by 59
From cells to organoids: The evolution of blood-brain barrier technology for modelling drug delivery in brain cancer
Gonzales‐Aloy, E., Ahmed-Cox, A., Tsoli, M., Ziegler, D. S., & Kavallaris, M. (2023) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2023.114777 · Cited by 60
AI-Validated Brain Targeted mRNA Lipid Nanoparticles with Neuronal Tropism
Sela, M., Chen, G., Kadosh, H., Kagan, T., Nicola, R., Turutov, S., Richtman, Y., Zhige, L., Albalak, M. R., Kagan, S., et al. (2025) · ACS Nano · doi:10.1021/acsnano.4c15013 · Cited by 63
Tailoring Lipid and Polymeric Nanoparticles as siRNA Carriers towards the Blood-Brain Barrier – from Targeting to Safe Administration
Gomes, M. J., Fernandes, C., Martins, S., Borges, F., & Sarmento, B. F. C. C. (2016) · Journal of Neuroimmune Pharmacology · doi:10.1007/s11481-016-9685-6 · Cited by 57
Development of a blood-brain barrier model in a membrane-based microchip for characterization of drug permeability and cytotoxicity for drug screening
Shao, X., Gao, D., Chen, Y., Jin, F., Hu, G., Jiang, Y., & Liu, H. (2016) · Analytica Chimica Acta · doi:10.1016/j.aca.2016.06.028 · Cited by 61
Designed Macrocyclic Peptides as Nanomolar Amyloid Inhibitors Based on Minimal Recognition Elements
Spanopoulou, A., Heidrich, L., Chen, H., Frost, C. V., Hrle, D., Malideli, E., Hille, K., Grammatikopoulos, A., Bernhagen, J., Zacharias, M., et al. (2018) · Angewandte Chemie International Edition · doi:10.1002/anie.201802979 · Cited by 57
A novel L1CAM isoform with angiogenic activity generated by NOVA2-mediated alternative splicing
Angiolini, F., Belloni, E., Giordano, M., Campioni, M., Forneris, F., Paronetto, M. P., Lupia, M., Brandas, C., Pradella, D., Matteo, A. D., et al. (2019) · eLife · doi:10.7554/elife.44305 · Cited by 58
Ultrathin Silicon Membranes for in Situ Optical Analysis of Nanoparticle Translocation across a Human Blood–Brain Barrier Model
Hudecz, D., Khire, T. S., Chung, H. L., Adumeau, L., Glavin, D., Luke, E., Nielsen, M. S., Dawson, K. A., McGrath, J. L., & Yan, Y. (2020) · ACS Nano · doi:10.1021/acsnano.9b08870 · Cited by 56
Angiopep-2-Functionalized Lipid Cubosomes for Blood–Brain Barrier Crossing and Glioblastoma Treatment
Cai, X., Refaat, A., Gan, P., Fan, B., Yu, H., Thang, S. H., Drummond, C. J., Voelcker, N. H., Tran, N. L., & Zhai, J. (2024) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.3c14709 · Cited by 56
CXCL1/CXCL8 (GROα/IL-8) in human diabetic ketoacidosis plasma facilitates leukocyte recruitment to cerebrovascular endothelium in vitro
Omatsu, T., Cepinskas, G., Clarson, C. L., Patterson, E. K., Alharfi, I. M., Summers, K. L., Couraud, P., Romero, I. A., Weksler, B. B., & Fraser, D. D. (2014) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00659.2013 · Cited by 56
Self-citation
Cys34-PEGylated Human Serum Albumin for Drug Binding and Delivery
Mehtala, J. G., Kulczar, C. D., Lavan, M., Knipp, G. T., & Wei, A. (2015) · Bioconjugate Chemistry · doi:10.1021/acs.bioconjchem.5b00143 · Cited by 57
Occludin regulates glucose uptake and ATP production in pericytes by influencing AMP-activated protein kinase activity
Castro, V., Skowrońska, M., Lombardi, J., He, J., Seth, N., Velichkovska, M., & Toborek, M. J. (2017) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1177/0271678x17720816 · Cited by 55
Assessment of HBEC-5i endothelial cell line cultivated in astrocyte conditioned medium as a human blood-brain barrier model for ABC drug transport studies
Puech, C., Hodin, S., Forest, V., Hé, Z., Mismetti, P., Delavenne, X., & Perek, N. (2018) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2018.09.040 · Cited by 54
Neutrophil-Derived Microvesicle Induced Dysfunction of Brain Microvascular Endothelial Cells In Vitro
Ajikumar, A., Long, M. B., Heath, P. R., Wharton, S. B., Ince, P. G., Ridger, V. C., & Simpson, J. E. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20205227 · Cited by 59
Region-specific blood–brain barrier transporter changes leads to increased sensitivity to amisulpride in Alzheimer’s disease
Sekhar, G. N., Fleckney, A. L., Boyanova, S., Rupawala, H., Lo, R., Wang, H., Farag, D. B., Rahman, K. M., Broadstock, M., Reeves, S., et al. (2019) · Fluids and Barriers of the CNS · doi:10.1186/s12987-019-0158-1 · Cited by 57
Brain Vascular Dysfunction in Mothers and Their Children Exposed to Preeclampsia
Escudero, C., Kupka, E., Ibáñez, B., Sandoval, H., Troncoso, F., Wikström, A., López-Espíndola, D., Acurio, J., Torres‐Vergara, P., & Bergman, L. (2022) · Hypertension · doi:10.1161/hypertensionaha.122.19408 · Cited by 53
Recent advances in blood-brain barrier-on-a-chip models
Vetter, J., Palagi, I., Waisman, A., & Blaeser, A. (2025) · Acta Biomaterialia · doi:10.1016/j.actbio.2025.03.041 · Cited by 58
Setting-up an In Vitro Model of Rat Blood-brain Barrier (BBB): A Focus on BBB Impermeability and Receptor-mediated Transport
Molino, Y., Jabès, F., Lacassagne, E., Gaudin, N., & Khrestchatisky, M. (2014) · Journal of Visualized Experiments · doi:10.3791/51278 · Cited by 69
Quantification of M13 and T7 bacteriophages by TaqMan and SYBR green qPCR
Peng, X., Nguyen, A., & Ghosh, D. (2017) · Journal of Virological Methods · doi:10.1016/j.jviromet.2017.11.012 · Cited by 55
Anti-TNFR1 targeting in humanized mice ameliorates disease in a model of multiple sclerosis
Williams, S. K., Fairless, R., Maier, O., Liermann, P. C., Pichi, K., Fischer, R., Eisel, U. L. M., Kontermann, R. E., Herrmann, A., Weksler, B. B., et al. (2018) · Scientific Reports · doi:10.1038/s41598-018-31957-7 · Cited by 55
Self-citation
Impact of metabolic disorders on the structural, functional, and immunological integrity of the blood‐brain barrier: Therapeutic avenues
Sheikh, M. H., Errede, M., d’Amati, A., Khan, N. Q., Fanti, S., Loiola, R. A., McArthur, S., Purvis, G. S. D., O’Riordan, C. E., Ferorelli, D., et al. (2021) · The FASEB Journal · doi:10.1096/fj.202101297r · Cited by 56
Transport of Gold Nanoparticles by Vascular Endothelium from Different Human Tissues
Gromnicova, R., Kaya, M., Romero, I. A., Williams, P., Satchell, S. C., Sharrack, B., & Male, D. K. (2016) · PLoS ONE · doi:10.1371/journal.pone.0161610 · Cited by 55
Self-citation
Antiretroviral Treatment with Efavirenz Disrupts the Blood-Brain Barrier Integrity and Increases Stroke Severity
Bertrand, L., Dygert, L., & Toborek, M. J. (2016) · Scientific Reports · doi:10.1038/srep39738 · Cited by 57
Real‐time acquisition of transendothelial electrical resistance in an all‐human, in vitro , 3‐dimensional, blood‐brain barrier model exemplifies tight‐junction integrity
Maherally, Z., Fillmore, H. L., Tan, S. L., Tan, S. F., Jassam, S. A., Quack, F., Hatherell, K. E., & Pilkington, G. J. (2017) · The FASEB Journal · doi:10.1096/fj.201700162r · Cited by 54
Nanotechnology-mediated crossing of two impermeable membranes to modulate the stars of the neurovascular unit for neuroprotection
Surnar, B., Basu, U., Banik, B., Ahmad, A., Marples, B., Kolishetti, N., & Dhar, S. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1816429115 · Cited by 53
Human Induced Pluripotent Stem Cell-Derived Brain Endothelial Cells: Current Controversies
Lu, T. M., Durán, J. G. B., Houghton, S., Rafii, S., Redmond, D., & Lis, R. (2021) · Frontiers in Physiology · doi:10.3389/fphys.2021.642812 · Cited by 54
Vascularized human brain organoid on-chip
Tan, S. Y., Feng, X., Cheng, L. K., & Wu, A. R. (2023) · Lab on a Chip · doi:10.1039/d2lc01109c · Cited by 52
In Vitro Cerebrovascular Modeling in the 21st Century: Current and Prospective Technologies
Palmiotti, C. A., Prasad, S., Naik, P. U., Kaisar, M. A., Sajja, R. K., Achyuta, A. H., & Cucullo, L. (2014) · Pharmaceutical Research · doi:10.1007/s11095-014-1464-6 · Cited by 56
Delivery of siRNA Silencing P-gp in Peptide-Functionalized Nanoparticles Causes Efflux Modulation at the Blood–Brain Barrier
Gomes, M. J., Kennedy, P. J., Martins, S., & Sarmento, B. F. C. C. (2017) · Nanomedicine · doi:10.2217/nnm-2017-0023 · Cited by 52
Evaluation of brain-targeted chitosan nanoparticles through blood–brain barrier cerebral microvessel endothelial cells
Şahin, A., Yöyen-Ermiş, D., Caban-Toktas, S., Horzum, U., Aktaş, Y., Couvreur, P., Esendağlı, G., & Çapan, Y. (2017) · Journal of Microencapsulation · doi:10.1080/02652048.2017.1375039 · Cited by 52
Preeclampsia and Increased Permeability Over the Blood–Brain Barrier: A Role of Vascular Endothelial Growth Receptor 2
Bergman, L., Acurio, J., Pino, J. M. L., Gatu, E., Friis, T., Nelander, M., Wikström, J., Larsson, A. O., Lara, E., Aguayo, C. R., et al. (2020) · American Journal of Hypertension · doi:10.1093/ajh/hpaa142 · Cited by 52
Extracellular Vesicles Derived from a Human Brain Endothelial Cell Line Increase Cellular ATP Levels
Dave, K. M., Zhao, W., Hoover, C. A., D’souza, A. A., & Manickam, D. S. (2021) · AAPS PharmSciTech · doi:10.1208/s12249-020-01892-w · Cited by 51
The interplay between glioblastoma and microglia cells leads to endothelial cell monolayer dysfunction via the interleukin‐6‐induced JAK2/STAT3 pathway
Couto, M., Coelho‐Santos, V., Santos, L., Ribeiro, C., Silva, A. P., & Gomes, C. M. F. (2019) · Journal of Cellular Physiology · doi:10.1002/jcp.28575 · Cited by 51
Heterocellular spheroids of the neurovascular blood-brain barrier as a platform for personalized nanoneuromedicine
Kumarasamy, M., & Sosnik, A. (2021) · iScience · doi:10.1016/j.isci.2021.102183 · Cited by 52
Hepatitis E virus infects brain microvascular endothelial cells, crosses the blood–brain barrier, and invades the central nervous system
Tian, D., Li, W., Heffron, C. L., Wang, B., Mahsoub, H. M., Sooryanarain, H., Hassebroek, A. M., Clark‐Deener, S., LeRoith, T., & Meng, X. (2022) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2201862119 · Cited by 51
FDA‐approved carbonic anhydrase inhibitors reduce amyloid β pathology and improve cognition, by ameliorating cerebrovascular health and glial fitness
Canepa, E., Parodi‐Rullán, R. M., Vázquez‐Torres, R., Gamallo‐Lana, B., Guzman‐Hernandez, R. A., Lemon, N. L., Angiulli, F., Debure, L., Ilies, M. A., Østergaard, L., et al. (2023) · Alzheimer s & Dementia · doi:10.1002/alz.13063 · Cited by 54
Generation of an alveolar epithelial type II cell line from induced pluripotent stem cells
Tamò, L., Hibaoui, Y., Kallol, S., Alves, M. P., Albrecht, C., Hostettler, K., Féki, A., Rougier, J., Abriel, H., Knudsen, L., et al. (2018) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00357.2017 · Cited by 48
In vivo biodistribution of venlafaxine-PLGA nanoparticles for brain delivery: plain vs. functionalized nanoparticles
Otero, M. D. C., Gomes, M. J., Martins, C., Álvarez‐Fuentes, J., Arévalo, M. F., Sarmento, B. F. C. C., & Martín‐Banderas, L. (2019) · Expert Opinion on Drug Delivery · doi:10.1080/17425247.2019.1690452 · Cited by 53
A Reconfigurable In Vitro Model for Studying the Blood–Brain Barrier
Moya, M. L., Triplett, M., Simon, M. G., Alvarado, J. A., Booth, R., Osburn, J. J., Soscia, D. A., Qian, F., Fischer, N. O., Kulp, K. S., et al. (2019) · Annals of Biomedical Engineering · doi:10.1007/s10439-019-02405-y · Cited by 51
RETRACTED: MiR-18a increased the permeability of BTB via RUNX1 mediated down-regulation of ZO-1, occludin and claudin-5
Miao, Y., Zhao, Y., Zhao, L., Wang, P., Liu, Y., Ma, J., & Xue, Y. (2014) · Cellular Signalling · doi:10.1016/j.cellsig.2014.10.008 · Cited by 49
Retracted
A Novel Blood-Brain Barrier Co-Culture System for Drug Targeting of Alzheimer’s Disease: Establishment by Using Acitretin as a Model Drug
Freese, C., Reinhardt, S., Hefner, G., Unger, R. E., Kirkpatrick, C. J., & Endres, K. (2014) · PLoS ONE · doi:10.1371/journal.pone.0091003 · Cited by 49
CC17 group B Streptococcus exploits integrins for neonatal meningitis development
de Cambronne, R. D., Fouet, A., Picart, A., Bourrel, A.-S., Anjou, C., Bouvier, G., Candeias, C., Bouaboud, A., Costa, L., Boulay, A.-C., et al. (2021) · Journal of Clinical Investigation · doi:10.1172/jci136737 · Cited by 50
Review of Design Considerations for Brain-on-a-Chip Models
Cameron, T., Bennet, T. J., Rowe, E. M., Anwer, M., Wellington, C. L., & Cheung, K. C. (2021) · Micromachines · doi:10.3390/mi12040441 · Cited by 49
Oncostatin M triggers brain inflammation by compromising blood–brain barrier integrity
Hermans, D., Houben, E., Baeten, P., Slaets, H., Janssens, K., Hoeks, C., Hosseinkhani, B., Duran, G., Bormans, S., Gowing, E., et al. (2022) · Acta Neuropathologica · doi:10.1007/s00401-022-02445-0 · Cited by 48
Protecting the BBB endothelium against cigarette smoke-induced oxidative stress using popular antioxidants: Are they really beneficial?
Kaisar, M. A., Prasad, S., & Cucullo, L. (2015) · Brain Research · doi:10.1016/j.brainres.2015.09.018 · Cited by 48
Regulation of Tight-Junction Integrity by Insulin in an In Vitro Model of Human Blood–Brain Barrier
Ito, S., Yanai, M., Yamaguchi, S., Couraud, P., & Ohtsuki, S. (2017) · Journal of Pharmaceutical Sciences · doi:10.1016/j.xphs.2017.04.036 · Cited by 49
Self-citation
Andrographolide-loaded nanoparticles for brain delivery: Formulation, characterisation and in vitro permeability using hCMEC/D3 cell line
Guccione, C., Oufir, M., Piazzini, V., Eigenmann, D. E., Jähne, E. A., Zabela, V., Faleschini, M. T., Bergonzi, M. C., Smieško, M., Hamburger, M. O., et al. (2017) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/j.ejpb.2017.06.018 · Cited by 48
Quantification of blood–brain barrier transport and neuronal toxicity of unlabelled multiwalled carbon nanotubes as a function of surface charge
Gonzalez‐Carter, D., Goode, A. E., Kiryushko, D. V., Masuda, S., Hu, S., Rodrigues, R. L., Dexter, D. T., Shaffer, M. S. P., & Porter, A. E. (2019) · Nanoscale · doi:10.1039/c9nr02866h · Cited by 48
Loss of miR-101-3p Promotes Transmigration of Metastatic Breast Cancer Cells through the Brain Endothelium by Inducing COX-2/MMP1 Signaling
Harati, R., Mohammad, M. G., Tlili, A., El‐Awady, R., & Hamoudi, R. (2020) · Pharmaceuticals · doi:10.3390/ph13070144 · Cited by 47
Induced expression of P-gp and BCRP transporters on brain endothelial cells using transferrin functionalized nanostructured lipid carriers: A first step of a potential strategy for the treatment of Alzheimer’s disease
Arduino, I., Iacobazzi, R. M., Riganti, C., Lopedota, A., Perrone, M. G., Lopalco, A., Cutrignelli, A., Cantore, M., Laquintana, V., Franco, M., et al. (2020) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2020.120011 · Cited by 47
Human placental mesenchymal stem cells improve stroke outcomes via extracellular vesicles-mediated preservation of cerebral blood flow
Barzegar, M., Wang, Y., Eshaq, R. S., Yun, J. W., Boyer, C. J., Cananzi, S. G., White, L. A., Chernyshev, O. Y., Kelley, R. E., Minagar, A., et al. (2020) · EBioMedicine · doi:10.1016/j.ebiom.2020.103161 · Cited by 48
Understanding the glioblastoma tumor biology to optimize photodynamic therapy: From molecular to cellular events
Ibarra, L. E., Vilchez, M. L., Caverzán, M. D., & Sanabria, L. N. M. (2020) · Journal of Neuroscience Research · doi:10.1002/jnr.24776 · Cited by 48
miR-27a-3p regulates expression of intercellular junctions at the brain endothelium and controls the endothelial barrier permeability
Harati, R., Hammad, S., Tlili, A., Mahfood, M., Mabondzo, A. M., & Hamoudi, R. (2022) · PLoS ONE · doi:10.1371/journal.pone.0262152 · Cited by 50
In vitroinfection models to study fungal–host interactions
Last, A., Maurer, M., Mosig, A. S., Gresnigt, M. S., & Hube, B. (2021) · FEMS Microbiology Reviews · doi:10.1093/femsre/fuab005 · Cited by 45
Modified cyclodextrin-based nanoparticles mediated delivery of siRNA for huntingtin gene silencing across an in vitro BBB model
Mendonça, M. C. P., Cronin, M. F., Cryan, J. F., & O’Driscoll, C. M. (2021) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/j.ejpb.2021.11.003 · Cited by 48
Effect of full flavor and denicotinized cigarettes exposure on the brain microvascular endothelium: a microarray-based gene expression study using a human immortalized BBB endothelial cell line
Naik, P. U., Sajja, R. K., Prasad, S., & Cucullo, L. (2015) · BMC Neuroscience · doi:10.1186/s12868-015-0173-3 · Cited by 45
Junctional adhesion molecules in cerebral endothelial tight junction and brain metastasis.
Jia, W., Martin, T. A., Zhang, G., & Jiang, W. G. (2013) · PubMed · Cited by 45
Uptake and transcytosis of functionalized superparamagnetic iron oxide nanoparticles in an in vitro blood brain barrier model
Ivask, A., Pilkington, E. H., Blin, T., Käkinen, A., Vija, H., Visnapuu, M., Quinn, J. F., Whittaker, M. R., Qiao, R., Davis, T. P., et al. (2017) · Biomaterials Science · doi:10.1039/c7bm01012e · Cited by 46
Paving the Way Toward Complex Blood-Brain Barrier Models Using Pluripotent Stem Cells
Lauschke, K., Frederiksen, L., & Hall, V. J. (2017) · Stem Cells and Development · doi:10.1089/scd.2017.0003 · Cited by 46
A multiparametric study of gold nanoparticles cytotoxicity, internalization and permeability using anin vitromodel of blood–brain barrier. Influence of size, shape and capping agent
Enea, M., de Almeida, M. P., Eaton, P. J., da Silva, D. D., Pereira, E., Soares, M. E., Bastos, M. D. L., & Carmo, H. (2019) · Nanotoxicology · doi:10.1080/17435390.2019.1621398 · Cited by 44
Microphysiological systems for recapitulating physiology and function of blood-brain barrier
Seo, S., Kim, H., Sung, J. H., Choi, N., Lee, K., & Kim, H. N. (2019) · Biomaterials · doi:10.1016/j.biomaterials.2019.119732 · Cited by 46
Biomedical engineering approaches to enhance therapeutic delivery for malignant glioma
McCrorie, P., Vasey, C. E., Smith, S. J., Marlow, M., Alexander, C., & Rahman, R. (2020) · Journal of Controlled Release · doi:10.1016/j.jconrel.2020.11.022 · Cited by 44
Edaravone Protects against Methylglyoxal-Induced Barrier Damage in Human Brain Endothelial Cells
Tóth, A. E., Walter, F. R., Bocsik, A., Sántha, P., Veszelka, S., Nagy, L. I., Puskás, L. G., Couraud, P., Takata, F., Dohgu, S., et al. (2014) · PLoS ONE · doi:10.1371/journal.pone.0100152 · Cited by 46
Self-citation
Understanding the Blood‐Brain Barrier and Beyond: Challenges and Opportunities for Novel CNS Therapeutics
de Lange, E. C. M., & Hammarlund‐Udenaes, M. (2022) · Clinical Pharmacology & Therapeutics · doi:10.1002/cpt.2545 · Cited by 47
Identification and Validation of Compounds Selectively Killing Resistant Cancer: Delineating Cell Line–Specific Effects from P-Glycoprotein–Induced Toxicity
Füredi, A., Tóth, S., Szebényi, K., Pape, V. F. S., Türk, D., Kucsma, N., Cervenak, L., Tóvári, J., & Szakács, G. (2016) · Molecular Cancer Therapeutics · doi:10.1158/1535-7163.mct-16-0333-t · Cited by 43
Blood-brain barrier disruption in diabetic mice is linked to Nrf2 signaling deficits: Role of ABCB10?
Sajja, R. K., Prasad, S., Tang, S., Kaisar, M. A., & Cucullo, L. (2017) · Neuroscience Letters · doi:10.1016/j.neulet.2017.05.059 · Cited by 43
Fabrication and Validation of an Organ-on-chip System with Integrated Electrodes to Directly Quantify Transendothelial Electrical Resistance
van der Helm, M. W., Odijk, M., Frimat, J., van der Meer, A. D., Eijkel, J. C. T., van den Berg, A., & Segerink, L. I. (2017) · Journal of Visualized Experiments · doi:10.3791/56334 · Cited by 43
Fine-tuning the neuroprotective and blood-brain barrier permeability profile of multi-target agents designed to prevent progressive mitochondrial dysfunction
Benfeito, S., Oliveira, C., Fernandes, C., Cagide, F., Teixeira, J., Amorim, R., Garrido, J. M. P. J., Martins, C., Sarmento, B. F. C. C., Silva, R. V. D. S., et al. (2019) · European Journal of Medicinal Chemistry · doi:10.1016/j.ejmech.2019.01.055 · Cited by 48
Cerebral Biomarkers and Blood-Brain Barrier Integrity in Preeclampsia
Friis, T., Wikström, A., Acurio, J., Pino, J. M. L., Zetterberg, H., Blennow, K., Nelander, M., Åkerud, H., Kaihola, H., Cluver, C. A., et al. (2022) · Cells · doi:10.3390/cells11050789 · Cited by 40
Uptake Transporters at the Blood–Brain Barrier and Their Role in Brain Drug Disposition
Parvez, M. M., Sadighi, A., Ahn, Y., Keller, S. F., & Enoru, J. O. (2023) · Pharmaceutics · doi:10.3390/pharmaceutics15102473 · Cited by 42
Multi-level analysis of gut microbiome extracellular vesicles-host interaction reveals a connection to gut-brain axis signaling
Mottawea, W., Yousuf, B., Sultan, S., Ahmed, T. A., Yeo, J., Hüttmann, N., Li, Y., Bouhlel, N. E., Hassan, H., Zhang, X., et al. (2024) · Microbiology Spectrum · doi:10.1128/spectrum.01368-24 · Cited by 45
miR‐34c Regulates the Permeability of Blood–Tumor Barrier via MAZ‐Mediated Expression Changes of ZO‐1, Occludin, and Claudin‐5
Zhao, L., Wang, P., Liu, Y., Ma, J., & Xue, Y. (2014) · Journal of Cellular Physiology · doi:10.1002/jcp.24799 · Cited by 42
H-Ferritin nanoparticle-mediated delivery of antibodies across a BBB in vitro model for treatment of brain malignancies
Rizzuto, M. A., Magro, R. D., Barbieri, L., Pandolfi, L., Sguazzini-Viscontini, A., Truffi, M., Salvioni, L., Corsi, F., Colombo, M., Re, F., et al. (2021) · Biomaterials Science · doi:10.1039/d0bm01726d · Cited by 42
Streptococcus suis serotype 2 enolase interaction with host brain microvascular endothelial cells and RPSA-induced apoptosis lead to loss of BBB integrity
Liu, H., Lei, S., Li, J., Xia, X., Sun, Y., Jiang, H., Zhu, R., Li, S., Qu, G., Gu, J., et al. (2021) · Veterinary Research · doi:10.1186/s13567-020-00887-6 · Cited by 40
Inflammation Induces Changes in the Functional Expression of P-gp, BCRP, and MRP2: An Overview of Different Models and Consequences for Drug Disposition
Saïb, S., & Delavenne, X. (2021) · Pharmaceutics · doi:10.3390/pharmaceutics13101544 · Cited by 42
Measuring barrier function in organ-on-chips with cleanroom-free integration of multiplexable electrodes
Bossink, E. G. B. M., Zakharova, M., de Bruijn, D. S., Odijk, M., & Segerink, L. I. (2021) · Lab on a Chip · doi:10.1039/d0lc01289k · Cited by 41
Nanoparticle Formulation Improves the Anticonvulsant Effect of Clonazepam on the Pentylenetetrazole-Induced Seizures: Behavior and Electroencephalogram
Leyva‐Gómez, G., González-Trujano, M. E., López-Ruiz, E., Couraud, P., Wekslerg, B., Romero, I. A., Miller, F., Delié, F., Allémann, É., & Quintanar‐Guerrero, D. (2014) · Journal of Pharmaceutical Sciences · doi:10.1002/jps.24044 · Cited by 41
Self-citation
Microparticles shed from multidrug resistant breast cancer cells provide a parallel survival pathway through immune evasion
Jaiswal, R., Johnson, M. S., Pokharel, D., Krishnan, S. R., & Bebawy, M. (2017) · BMC Cancer · doi:10.1186/s12885-017-3102-2 · Cited by 41
Synthesis and properties of a series of carboranyl-BODIPYs
Gibbs, J. H., Wang, H., Bhupathiraju, N. V. S. D. K., Fronczek, F. R., Smith, K. M., & Vicente, M. G. H. (2015) · Journal of Organometallic Chemistry · doi:10.1016/j.jorganchem.2015.05.009 · Cited by 41
Arachidonic Acid Evokes an Increase in Intracellular Ca2+ Concentration and Nitric Oxide Production in Endothelial Cells from Human Brain Microcirculation
Berra‐Romani, R., Faris, P. S., Negri, S., Botta, L., Genova, T., & Moccia, F. D. (2019) · Cells · doi:10.3390/cells8070689 · Cited by 42
Bacteriophage K1F targets Escherichia coli K1 in cerebral endothelial cells and influences the barrier function
Møller-Olsen, C., Ross, T., Leppard, K. N., Foisor, V., Smith, C. J., Grammatopoulos, D. K., & Sagona, A. P. (2020) · Scientific Reports · doi:10.1038/s41598-020-65867-4 · Cited by 41
Silencing miR-202-3p increases MMP-1 and promotes a brain invasive phenotype in metastatic breast cancer cells
Harati, R., Hafezi, S., Mabondzo, A. M., & Tlili, A. (2020) · PLoS ONE · doi:10.1371/journal.pone.0239292 · Cited by 40
Dysregulation of Endoplasmic Reticulum Stress and Autophagic Responses by the Antiretroviral Drug Efavirenz
Bertrand, L., & Toborek, M. J. (2015) · Molecular Pharmacology · doi:10.1124/mol.115.098590 · Cited by 43
Cracking the Blood–Brain Barrier Code: Rational Nanomaterial Design for Next-Generation Neurological Therapies
Nájera-Maldonado, L., Parra-González, M., Peralta-Cuevas, E., Gutierrez-Onofre, A. J., Garcia-Atutxa, I., & Villanueva-Flores, F. (2025) · Pharmaceutics · doi:10.3390/pharmaceutics17091169 · Cited by 40
The endo-lysosomal system of bEnd.3 and hCMEC/D3 brain endothelial cells
Toth, A. E., Nielsen, S. S. E., Tomaka, W., Abbott, N. J., & Nielsen, M. S. (2019) · Fluids and Barriers of the CNS · doi:10.1186/s12987-019-0134-9 · Cited by 40
Quantitative Targeted Absolute Proteomics for Better Characterization of an In Vitro Human Blood–Brain Barrier Model Derived from Hematopoietic Stem Cells
Dehouck, M., Tachikawa, M., Hoshi, Y., Omori, K., Maurage, C., Strecker, G., Dehouck, L., Boucau, M., Uchida, Y., Gosselet, F., et al. (2022) · Cells · doi:10.3390/cells11243963 · Cited by 41
Implementation of blood-brain barrier on microfluidic chip: Recent advance and future prospects
Bi, W., Cai, S., Lei, T., & Wang, L. (2023) · Ageing Research Reviews · doi:10.1016/j.arr.2023.101921 · Cited by 44
BBBomics-Human Blood Brain Barrier Transcriptomics Hub
Kalari, K. R., Thompson, K. J., Nair, A., Tang, X., Bockol, M. A., Jhawar, N., Swaminathan, S. K., Lowe, V. J., & Kandimalla, K. K. (2016) · Frontiers in Neuroscience · doi:10.3389/fnins.2016.00071 · Cited by 40

Authors: Weksler, B., Romero, I. A., & Couraud, P.-O.
Affiliations: Inserm (FR)
The Open University (GB)
Centre National de la Recherche Scientifique (FR)
Corresponding author: Couraud, Pierre-Olivier
ORCID: Weksler, Babette
Romero, Ignacio A
Venue: Fluids and Barriers of the CNS
Publisher: Springer Science and Business Media LLC
Published: 2013-03-26
Volume: 10
Issue: 1
Pages: 16-16
ISSN: 2045-8118 (ISSN-L)
DOI: 10.1186/2045-8118-10-16
PMC: PMC3623852
OpenAlex: W2115924220
Cited by: 753
References: 77
Citations by year: 2026: 45 · 2025: 70 · 2024: 76 · 2023: 75 · 2022: 68 · 2021: 83 · 2020: 58 · 2019: 63 · 2018: 36 · 2017: 53 · 2016: 42 · 2015: 36 · 2014: 36 · 2013: 10
FWCI: 23.39
Type: journal article
Language: en
Open access: gold
doi.org
Licence: cc-by
Locations: Fluids and Barriers of the CNS (publishedVersion, cc-by)
Europe PMC (PubMed Central) (publishedVersion, cc-by)
PubMed Central (publishedVersion, cc-by)
PubMed (publishedVersion)
Open Research Online - ORO (The Open University) (acceptedVersion)
HAL (submittedVersion, other-oa)
Topics: Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
Keywords: blood-brain barrier, in vitro BBB model, drug transport across the blood-brain barrier, brain drug delivery, endothelial cell biology

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

The hCMEC/D3 cell line as a model of the human blood brain barrier

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks