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miR-96, miR-145 and miR-9 expression increases, and IGF-1R and FOXO1 expression decreases in peripheral blood mononuclear cells of aging humans

Budzinska, Monika, Owczarz, Magdalena, Pawlik-Pachucka, Eliza, Roszkowska-Gancarz, Malgorzata, Slusarczyk, Przemyslaw, Puzianowska-Kuznicka, Monika·2016·BMC Geriatrics
DOI 10.1186/s12877-016-0379-yPMC PMC5131432
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Fig. 1 miR-96 and miR-182 interact with the 3’UTR of IGF-1R mRNA. HEK 293 cells were co-transfected with the pmirGLO reporter vector and miRNA precursors. The mean relative luminescence induced by firefly luciferase expressed from the reporter vectors containing cloned DNA corresponding to the 3’UTR fragments, in the presence of negative control miRNA, was normalized to 100%. miR-96 interacts with two (a, b), and miR-182 with one out of two in silico indicated binding sites (c, d). pmirGLO: “empty” reporter vector; pmirGLO_IGF-1R_5’: reporter vector containing DNA corresponding to the 5’ end of 3’UTR of IGF-1R mRNA; pmirGLO_IGF-1R_3’: reporter vector containing DNA corresponding to the 3’ end of 3’UTR of IGF-1R mRNA; pre-miR-neg, pre-miR-96, pre-miR-182: miRNA precursors
Fig. 1 miR-96 and miR-182 interact with the 3’UTR of IGF-1R mRNA. HEK 293 cells were co-transfected with the pmirGLO reporter vector and miRNA precursors. The mean relative luminescence induced by firefly luciferase expressed from the reporter vectors containing cloned DNA corresponding to the 3’UTR fragments, in the presence of negative control miRNA, was normalized to 100%. miR-96 interacts with two (a, b), and miR-182 with one out of two in silico indicated binding sites (c, d). pmirGLO: “empty” reporter vector; pmirGLO_IGF-1R_5’: reporter vector containing DNA corresponding to the 5’ end of 3’UTR of IGF-1R mRNA; pmirGLO_IGF-1R_3’: reporter vector containing DNA corresponding to the 3’ end of 3’UTR of IGF-1R mRNA; pre-miR-neg, pre-miR-96, pre-miR-182: miRNA precursorsCC BY · PubMed Central
Fig. 2 miR-145 and miR-132 interact with the 3’UTR of FOXO1 mRNA. HEK 293 cells were co-transfected with the pmirGLO reporter vector and miRNA precursors. The mean relative luminescence induced by firefly luciferase expressed from the reporter vectors containing cloned 3’UTR fragments in the presence of negative control miRNA was normalized to 100%. Both miR-145 (a) and miR-132 (b) interact with in silico indicated binding sites. pmirGLO: “empty” reporter vector; pmirGLO_FOXO1_5’: reporter vector containing DNA corresponding to the 5’ end of 3’UTR of FOXO1 mRNA; pmirGLO_FOXO1_3’: reporter vector containing DNA corresponding to the 3’ end of 3’UTR of FOXO1 mRNA; pre-miR-neg, pre-miR-145, pre-miR-132: miRNA precursors
Fig. 2 miR-145 and miR-132 interact with the 3’UTR of FOXO1 mRNA. HEK 293 cells were co-transfected with the pmirGLO reporter vector and miRNA precursors. The mean relative luminescence induced by firefly luciferase expressed from the reporter vectors containing cloned 3’UTR fragments in the presence of negative control miRNA was normalized to 100%. Both miR-145 (a) and miR-132 (b) interact with in silico indicated binding sites. pmirGLO: “empty” reporter vector; pmirGLO_FOXO1_5’: reporter vector containing DNA corresponding to the 5’ end of 3’UTR of FOXO1 mRNA; pmirGLO_FOXO1_3’: reporter vector containing DNA corresponding to the 3’ end of 3’UTR of FOXO1 mRNA; pre-miR-neg, pre-miR-145, pre-miR-132: miRNA precursorsCC BY · PubMed Central

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Metadata

Authors
Budzinska, M., Owczarz, M., Pawlik-Pachucka, E., Roszkowska-Gancarz, M., Slusarczyk, P., & Puzianowska-Kuznicka, M.
Venue
BMC Geriatrics
Publisher
Springer Science and Business Media LLC
Published
2016-11-30
Volume
16
Issue
1
ISSN
1471-2318
DOI
10.1186/s12877-016-0379-y
PMC
PMC5131432
Cited by
29
References
8
Language
en
Licence
www.springer.com/tdm (unspecified)
Funders
Polish Ministry of Science and Higher Education (NN401037338)

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