Cited by 68Medicinal Plants and NeuroprotectionNeurological Disorders and TreatmentsNeurological Disease Mechanisms and Treatments

Neuroprotective effects of Liriope platyphylla extract against hydrogen peroxide-induced cytotoxicity in human neuroblastoma SH-SY5Y cells

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Park, Hee Ra, Lee, Heeeun, Park, Hwayong, Jeon, Jong Wook, Cho, Won-Kyung, Ma, Jin Yeul·2015·BMC Complementary and Alternative Medicine

Abstract

BACKGROUND: Oxidative stress is involved in neuronal cell death and mitochondrial dysfunction in neurodegenerative diseases. Liriope platyphylla (LP) has been suggested to have anti-inflammation, anti-bacterial, and anti-cancer effects. However, whether LP exerts neuroprotective effects on neuronal cells is unknown. METHODS: The present study was performed to investigate the neuroprotective effects of LP extract (LPE) against hydrogen peroxide (H2O2)-induced injury in human neuroblastoma cells SH-SY5Y. To test neuroprotective effects of LPE, we performed cell viability assay, flow cytometry analysis and western blot analysis. In addition, mitochondrial membrane potential (MMP) and oxidative stress were performed to evaluate the anti-apoptotic and anti-oxidant effects. RESULTS: LPE pretreatment conferred significant protection against the H2O2-induced decrease of SH-SY5Y cell viability. H2O2-induced increases of intracellular oxidative stress and mitochondrial dysfunction were attenuated by LPE pretreatment. Therefore, LPE pretreatment prevented SH-SY5Y cell injury. Treatment with H2O2 significantly induced poly(ADP ribose) polymerase (PARP) and caspase-3 cleavage, which was blocked by LPE. We found that p38 activation was involved in the neuroprotective effects of LPE. CONCLUSIONS: Current findings suggest that LPE exerts neuroprotective effects against H2O2-induced apoptotic cell death by modulating p38 activation in SH-SY5Y cells. Therefore, LPE has potential anti-apoptotic effects that may be neuroprotective in neurodegenerative diseases and aging-related dementia.

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Authors
Park, H. R., Lee, H., Park, H., Jeon, J. W., Cho, W.-K., & Ma, J. Y.
Affiliations
Korea Institute of Oriental Medicine (KR)
Venue
BMC Complementary and Alternative Medicine
Published
2015-06-09
Volume
15
Issue
1
Pages
171-171
ISSN
1472-6882 (ISSN-L)
OpenAlex
W1533508825
Cited by
68
References
57
Citations by year
2025: 1 · 2024: 6 · 2023: 5 · 2022: 9 · 2021: 6 · 2020: 8 · 2019: 6 · 2018: 10 · 2017: 10 · 2016: 7
FWCI
17.66
Type
journal article
Language
en
Open access
Locations
  • BMC Complementary and Alternative Medicine (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
Korea Institute of Oriental Medicine (K15280)
Topics
  • Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
  • Life Sciences › Neuroscience › Neurology › Neurological Disorders and Treatments
  • Life Sciences › Neuroscience › Neurology › Neurological Disease Mechanisms and Treatments
Keywords
Liriope platyphylla, neuroprotection, hydrogen peroxide, oxidative stress, apoptosis, mitochondrial dysfunction, mitochondrial membrane potential, p38 MAPK, caspase-3, PARP cleavage, SH-SY5Y cells, neurodegenerative diseases, plant extracts
MeSH terms
Antioxidants, Cell Survival, Dementia, Humans, Hydrogen Peroxide, Phytotherapy, Mitochondria, Neuroblastoma, Neurons, Plant Extracts, Poly(ADP-ribose) Polymerases, Cell Death, Apoptosis, Oxidative Stress, Neuroprotective Agents, Neurodegenerative Diseases, Liliaceae, Cell Line, Tumor, Membrane Potential, Mitochondrial, Caspase 3

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