Neuroprotective Effects of Bioavailable Polyphenol-Derived Metabolites against Oxidative Stress-Induced Cytotoxicity in Human Neuroblastoma SH-SY5Y Cells
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Abstract Oxidative stress is involved in cell death in neurodegenerative diseases. Dietary polyphenols can exert health benefits, but their direct effects on neuronal cells are debatable because most phenolics are metabolized and do not reach the brain as they occur in the dietary sources. Herein, we evaluate the effects of a panel of bioavailable polyphenols and derived metabolites at physiologically relevant conditions against H2O2-induced apoptosis in human neuroblastoma SH-SY5Y cells. Among the 19 metabolites tested, 3,4-dihydroxyphenylpropionic acid, 3,4-dihydroxyphenylacetic acid, gallic acid, ellagic acid, and urolithins prevented neuronal apoptosis via attenuation of ROS levels, increased REDOX activity, and decreased oxidative stress-induced apoptosis by preventing the caspase-3 activation via the mitochondrial apoptotic pathway in SH-SY5Y cells. This suggests that dietary sources containing the polyphenol precursors of these molecules such as cocoa, berries, walnuts, and tea could be potential functional foods to reduce oxidative stress associated with the onset and progress of neurodegenerative diseases.
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The human neuroblastoma SH-SY5Y is among the most used cell lines, a subclone of the parental neuronal line SK-N-SH, which originated in 1970 from a biopsy metastatic tumor.
“The human neuroblastoma SH-SY5Y is among the most used cell lines [131,136,137,138,139,140,141,142], a subclone of a parental neuronal line SK-N-SH, which originated in 1970 from a biopsy metastatic tumor.”
From Multi-Target Mechanisms of Phytochemicals in Alzheimer’s Disease: Effects on Oxidative Stress, Neuroinflammation and Protein Aggregation (2022) · cites this paper as [138]
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The gut microbiota: a key factor in the therapeutic effects of (poly)phenols
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- Authors
- González-Sarrías, A., Núñez-Sánchez, M. A., Tomás-Barberán, F. A., & Espín, J. C.
- Affiliations
- CEBAS-CSIC , , P.O. Box 164, , ,
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2016-11-14
- Volume
- 65
- Issue
- 4
- Pages
- 752-758
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2556479552
- Cited by
- 174
- References
- 48
- Citations by year
- 2026: 15 · 2025: 12 · 2024: 15 · 2023: 12 · 2022: 28 · 2021: 24 · 2020: 21 · 2019: 17 · 2018: 19 · 2017: 11
- FWCI
- 12.28
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion, other-oa)
- PubMed (publishedVersion)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion, other-oa)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion)
- Funders
- Ministerio de Economía y Competitividad (NA)
- Consejo Superior de Investigaciones Científicas (201370E068)
- Seventh Framework Programme (312090)
- Comisión Interministerial de Ciencia y Tecnología (AGL2011-22447, AGL2015-64124-R)
- Topics
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Health Sciences › Nursing › Nutrition and Dietetics › Pomegranate: compositions and health benefits
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Keywords
- polyphenol metabolites, oxidative stress, neuroprotection, apoptosis, SH-SY5Y cells, hydrogen peroxide, reactive oxygen species, caspase-3, mitochondrial apoptosis, DOPAC, gallic acid, ellagic acid, urolithins, neurodegenerative diseases, functional foods, dietary polyphenols
- MeSH terms
- Humans, Neurons, Molecular Structure, Apoptosis, Oxidative Stress, Neuroprotective Agents, Cell Line, Tumor, Caspase 3, Polyphenols
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