The DOI matches its Crossref record; 53 of the 53 references it cites were found in a registry or an index.
Development of Dirofilaria immitis within the mosquito Aedes (Finlaya) koreicus, a new invasive species for Europe
Open accessAbstract
BACKGROUND: Over the recent decades, container-breeding mosquito species belonging to the genus Aedes have frequently been recorded far from their place of origin. Aedes koreicus was first reported in north-eastern Italy in 2011, in a region endemic for Dirofilaria immitis, the agent of canine heartworm disease. The vector competence of Ae. koreicus for D. immitis was here tested under laboratory conditions, by infecting mosquitoes with a local strain of D. immitis. METHODS: Blood containing 3000 microfilariae/ml was offered to 54 mosquitoes (T group) while 29 were left as a control (C group). Mosquitoes killed at scheduled days post infection (dpi) and naturally dead were divided in head, thorax and abdomen and examined for D. immitis larval stages by dissection under a microscope and molecularly. RESULTS: Of the 45 engorged mosquitoes in T, 32 (71.1%) scored positive for D. immitis larval stages. L3 were found as early as 8 dpi in the Malpighian tubules and then in the thorax, salivary glands, palp and proboscis. At the end of the study a total of 18 mosquitoes developed L3 giving an estimated infection rate at 12 dpi of 68.2% and a vector efficiency index of 25.2%. The rate of mortality in T group within the first 9 days post infection was significantly higher in T group (47.6%) than in C group (8.3%) (p < 0.01). The concordance between microscopy and PCR was high (0.8-0.9), however, a positivity for D. immitis in the head was found molecularly at 13 dpi, three days before microscopy. CONCLUSIONS: Aedes koreicus, a new invasive species for Europe, is most likely a competent vector of D. immitis being of potential relevance in the natural cycle of the parasite. This poses a new threat for animal and human health in endemic areas for dirofilariosis and enhances the risk of spreading the infection in previously non-endemic areas. These results stress the importance of active surveillance and control strategies to minimize the risk of introduction of invasive alien species.
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“Moreover, a new vector was detected in Europe, Ae. koreicus, which was identified in the Province of Belluno, north-eastern Italy, and in Chiasso, in the Swiss–Italian border region [152,153].”
From What has happened to heartworm disease in Europe in the last 10 years? (2022) · cites this paper as [152]
In the citing paper
“D. immitis has spread in the last years in Europe due to climate change, the spreading of mosquito Aedes albopictus and the introduction of new vector Aedes koreicus [29].”
From Dirofilaria immitis and Angiostrongylus vasorum: the contemporaneous detection in kennels (2015) · cites this paper as [29]
In the citing paper
“Despite the large natural infestation of European mosquitoes from endemic areas [5–22], only a few species have been experimentally confirmed as competent for Dirofilaria spp. (e.g., Cx. pipiens s.l., Ae. albopictus, Aedes aegypti, Aedes japonicus, Aedes geniculatus and Aedes koreicus) [47–50].”
From Dirofilaria immitis and Dirofilaria repens in mosquitoes from Corsica Island, France (2021) · cites this paper as [48]
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The prevalence of Dirofilaria immitis and D. repens in the Old World
Genchi, C., & Kramer, L. H. (2020) · Veterinary Parasitology · doi:10.1016/j.vetpar.2019.108995 · Cited by 187
Subcutaneous dirofilariosis (Dirofilaria repens): an infection spreading throughout the old world
Genchi, C., & Kramer, L. (2017) · Parasites & Vectors · doi:10.1186/s13071-017-2434-8 · Cited by 159
Parasite Ecology of Invasive Species: Conceptual Framework and New Hypotheses
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Heartworm disease – Overview, intervention, and industry perspective
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Metadata
- Authors
- Montarsi, F., Ciocchetta, S., Devine, G., Ravagnan, S., Mutinelli, F., Frangipane di Regalbono, A., Otranto, D., & Capelli, G.
- Affiliations
- Istituto Zooprofilattico Sperimentale delle Venezie (IT)
- Queensland University of Technology (AU)
- QIMR Berghofer Medical Research Institute (AU)
- University of Bari Aldo Moro (IT)
- Corresponding author
- Capelli, Gioia
- ORCID
- Venue
- Parasites & Vectors
- Published
- 2015-03-23
- Volume
- 8
- Issue
- 1
- Pages
- 177-177
- ISSN
- 1756-3305 (ISSN-L)
- PMC
- PMC4382832
- OpenAlex
- W2126586237
- Cited by
- 101
- References
- 53
- Citations by year
- 2026: 6 · 2025: 3 · 2024: 4 · 2023: 8 · 2022: 22 · 2021: 12 · 2020: 8 · 2019: 8 · 2018: 8 · 2017: 12 · 2016: 6 · 2015: 4
- FWCI
- 7.80
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Parasites & Vectors (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- QUT ePrints (Queensland University of Technology) (submittedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- CINECA IRIS Institutional Research Information System (University of Bari Aldo Moro) (submittedVersion, other-oa)
- Padua Research Archive (University of Padova) (submittedVersion, cc-by)
- Funders
- Bayer HealthCare
- Ministero della Salute
- Regione del Veneto
- Topics
- Health Sciences › Medicine › Infectious Diseases › Parasitic Diseases Research and Treatment
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Mosquito-borne diseases and control
- Life Sciences › Immunology and Microbiology › Parasitology › Vector-borne infectious diseases
- Keywords
- Dirofilaria immitis, Aedes koreicus, vector competence, canine heartworm disease, invasive mosquito species, mosquito mortality, Malpighian tubules, salivary glands, dirofilariasis, invasive species, Europe
- MeSH terms
- Aedes, Animals, Dirofilaria immitis, Dirofilariasis, Dog Diseases, Dogs, Europe, Humans, Insect Vectors, Italy, Larva, Introduced Species
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