Cytochrome C Oxidase in Alzheimer's Disease Brain: Purification and Characterization
Abstract
Cytochrome c oxidase (COX) is deficient in both peripheral tissue and brain of Alzheimer's disease (AD) patients and may be of pathogenic significance in AD. We purified COX from AD brains (n = 3) and control brains (n = 3) and characterized the enzyme kinetically and spectrally. Purified AD brain COX displayed anomalous kinetic behavior compared with control brain COX in that the low Km binding site was kinetically unidentifiable. For purposes of comparison, we purified COX from a standard beef heart preparation and found normal kinetic behavior. AD brain COX may be structurally abnormal and may make an important contribution to the bioenergetic defect seen in AD.
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Brain mitochondria in AD patients have dysfunctional respiratory chain complexes and decreased ATP generation.
“Brain mitochondria in AD patients have shown dysfunctional respiratory chain complexes and decreased ATP generation”
From Apoe4 and Alzheimer’s Disease Pathogenesis—Mitochondrial Deregulation and Targeted Therapeutic Strategies (2023) · cites this paper as [58]
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- Authors
- Parker, W. D., & Parks, J. K.
- Affiliations
- University of Virginia (US)
- Venue
- Neurology
- Published
- 1995-03
- Volume
- 45
- Issue
- 3
- Pages
- 482-486
- ISSN
- 0028-3878 (ISSN-L)
- 1526-632X
- OpenAlex
- W2059543204
- Cited by
- 142
- References
- 1
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 2 · 2023: 7 · 2022: 3 · 2021: 9 · 2020: 5 · 2019: 5 · 2018: 3 · 2017: 3 · 2016: 7 · 2015: 2 · 2013: 5 · 2012: 8
- FWCI
- 3.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neurology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute on Aging (AG09417, P01AG009417)
- Topics
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Coenzyme Q10 studies and effects
- Keywords
- cytochrome c oxidase, Alzheimer's disease, enzyme kinetics, enzyme purification, brain bioenergetics, structural abnormalities
- MeSH terms
- Aged, Aged, 80 and over, Alzheimer Disease, Binding Sites, Brain, Electron Transport Complex IV, Humans, Kinetics, Middle Aged, Polarography, Spectrophotometry
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