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Cited by 45melanin and skin pigmentationDevelopmental Biology and Gene RegulationZebrafish Biomedical Research Applications

Heterotypic interactions regulate cell shape and density during color pattern formation in zebrafish

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Mahalwar, Prateek, Singh, Ajeet Pratap, Fadeev, Andrey, Nüsslein-Volhard, Christiane, Irion, Uwe·2016·Biology Open
DOI 10.1242/bio.022251PMC PMC5155543
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Abstract

The conspicuous striped coloration of zebrafish is produced by cell-cell interactions among three different types of chromatophores: black melanophores, orange/yellow xanthophores and silvery/blue iridophores. During color pattern formation xanthophores undergo dramatic cell shape transitions and acquire different densities, leading to compact and orange xanthophores at high density in the light stripes, and stellate, faintly pigmented xanthophores at low density in the dark stripes. Here, we investigate the mechanistic basis of these cell behaviors in vivo, and show that local, heterotypic interactions with dense iridophores regulate xanthophore cell shape transition and density. Genetic analysis reveals a cell-autonomous requirement of gap junctions composed of Cx41.8 and Cx39.4 in xanthophores for their iridophore-dependent cell shape transition and increase in density in light-stripe regions. Initial melanophore-xanthophore interactions are independent of these gap junctions; however, subsequently they are also required to induce the acquisition of stellate shapes in xanthophores of the dark stripes. In summary, we conclude that, whereas homotypic interactions regulate xanthophore coverage in the skin, their cell shape transitions and density is regulated by gap junction-mediated, heterotypic interactions with iridophores and melanophores.

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  • The authors' data, together with those of others, suggest that some of these signals depend on melanophores, promoting differentiation of iridoblasts toward a state having ordered reflecting crystal platelets that are physiologically responsive (blue ⟷ yellow) in stripes, and away from an alternative state of disordered-crystal platelets lacking physiological responsiveness (silvery-yellow) in the

    “Our data, together with those of others, suggest that some of these signals depend on melanophores14,15,25,47, promoting differentiation of iridoblasts toward a state having ordered reflecting crystal platelets that are physiologically responsive (blue ⟷ yellow) in stripes, and away from an alternative state of disordered-crystal platelets lacking physiological responsiveness (silvery-yellow) in”

    From In situ differentiation of iridophore crystallotypes underlies zebrafish stripe patterning (2020) · cites this paper as [47]

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Authors
Mahalwar, P., Singh, A. P., Fadeev, A., Nüsslein-Volhard, C., & Irion, U.
Affiliations
  • Max Planck Institute for Developmental Biology (DE)
  • Max Planck Institute for Developmental Biology, Spemannstrasse 35, Tübingen 72076, Germany
Corresponding author
  • Mahalwar, Prateek
  • Irion, Uwe
ORCID
Venue
Biology Open
Publisher
The Company of Biologists
Published
2016-11-15
Volume
5
Issue
11
Pages
1680-1690
ISSN
2046-6390 (ISSN-L)
DOI
10.1242/bio.022251
PMC
PMC5155543
OpenAlex
W2531189335
Cited by
45
References
1
Citations by year
2025: 2 · 2024: 3 · 2023: 7 · 2021: 8 · 2020: 9 · 2019: 6 · 2018: 7 · 2017: 3
FWCI
3.67
Type
journal article
Language
en
Open access
Licence
Locations
  • Biology Open (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
  • Max Planck Institute for Plasma Physics (submittedVersion)
  • Max Planck Digital Library (submittedVersion)
  • HighWire Press Open Archive (submittedVersion)
  • MPG.PuRe (Max Planck Society) (submittedVersion, other-oa)
Funders
Max-Planck Society
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
Keywords
xanthophores, iridophores, melanophores, cell density, gap junctions

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