The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Intraflagellar transport is essential for endochondral bone formation
Open accessAbstract
While cilia are present on most cells in the mammalian body, their functional importance has only recently been discovered. Cilia formation requires intraflagellar transport (IFT), and mutations disrupting the IFT process result in loss of cilia and mid-gestation lethality with developmental defects that include polydactyly and abnormal neural tube patterning. The early lethality in IFT mutants has hindered research efforts to study the role of this organelle at later developmental stages. Thus, to investigate the role of cilia during limb development, we generated a conditional allele of the IFT protein Ift88 (polaris). Using the Cre-lox system, we disrupted cilia on different cell populations within the developing limb. While deleting cilia in regions of the limb ectoderm had no overt effect on patterning, disruption in the mesenchyme resulted in extensive polydactyly with loss of anteroposterior digit patterning and shortening of the proximodistal axis. The digit patterning abnormalities were associated with aberrant Shh pathway activity, whereas defects in limb outgrowth were due in part to disruption of Ihh signaling during endochondral bone formation. In addition, the limbs of mesenchymal cilia mutants have ectopic domains of cells that resemble chondrocytes derived from the perichondrium, which is not typical of Indian hedgehog mutants. Overall these data provide evidence that IFT is essential for normal formation of the appendicular skeleton through disruption of multiple signaling pathways.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Vertebrate Smoothened functions at the primary cilium
Corbit, K. C., Aanstad, P., Singla, V., Norman, A. R., Stainier, D. Y. R., & Reiter, J. F. (2005) · Nature · doi:10.1038/nature04117 · Cited by 1,481
Bitgood, M. J., & McMahon, A. P. (1995) · Developmental Biology · doi:10.1006/dbio.1995.0010 · Cited by 1,370
Pazour, G. J., Dickert, B. L., Vucica, Y., Seeley, E. S., Rosenbaum, J. L., Witman, G. B., & Cole, D. G. (2000) · The Journal of Cell Biology · doi:10.1083/jcb.151.3.709 · Cited by 1,147
Wang, B., Fallon, J. F., & Beachy, P. A. (2000) · Cell · doi:10.1016/s0092-8674(00)80678-9 · Cited by 1,014
Haycraft, C. J., Banizs, B., Aydin-Son, Y., Zhang, Q., Michaud, E. J., & Yoder, B. K. (2005) · PLoS Genetics · doi:10.1371/journal.pgen.0010053 · Cited by 902
Self-citationCole, D. G., Diener, D. R., Himelblau, A. L., Beech, P. L., Fuster, J. C., & Rosenbaum, J. L. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.141.4.993 · Cited by 898
Cilia and Hedgehog responsiveness in the mouse
Huangfu, D., & Anderson, K. V. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0505328102 · Cited by 820
Simons, M., Gloy, J., Ganner, A., Bullerkotte, A., Bashkurov, M., Krönig, C., Schermer, B., Benzing, T., Cabello, O. A., Jenny, A., et al. (2005) · Nature Genetics · doi:10.1038/ng1552 · Cited by 767
Kozminski, K. G., Beech, P. L., & Rosenbaum, J. L. (1995) · The Journal of cell biology · doi:10.1083/jcb.131.6.1517 · Cited by 586
Cited by
Kinesin superfamily motor proteins and intracellular transport
Hirokawa, N., Noda, Y., Tanaka, Y., & Niwa, S. (2009) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2774 · Cited by 1,859
Ciliopathies: an expanding disease spectrum
Waters, A. M., & Beales, P. L. (2011) · Pediatric Nephrology · doi:10.1007/s00467-010-1731-7 · Cited by 676
Primary Cilia and Mammalian Hedgehog Signaling
Bangs, F., & Anderson, K. V. (2017) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a028175 · Cited by 642
Hedgehog signaling and primary cilia are required for the formation of adult neural stem cells
Han, Y.-G., Spassky, N., Romaguera-Ros, M., Garcia-Verdugo, J.-M., Aguilar, A., Schneider-Maunoury, S., & Alvarez-Buylla, A. (2008) · Nature Neuroscience · doi:10.1038/nn2059 · Cited by 547
Davenport, J. R., Watts, A. J., Roper, V. C., Croyle, M. J., van Groen, T., Wyss, J. M., Nagy, T. R., Kesterson, R. A., & Yoder, B. K. (2007) · Current Biology · doi:10.1016/j.cub.2007.08.034 · Cited by 482
Self-citationMotile and non-motile cilia in human pathology: From function to phenotypes
Mitchison, H. M., & Valente, E. M. (2017) · The Journal of Pathology · doi:10.1002/path.4843 · Cited by 448
Primary cilia mediate mechanosensing in bone cells by a calcium-independent mechanism
Malone, A. M. D., Anderson, C. T., Tummala, P., Kwon, R. Y., Johnston, T. R., Stearns, T., & Jacobs, C. R. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0700636104 · Cited by 477
Beckervordersandforth, R., Tripathi, P., Ninkovic, J., Bayam, E., Lepier, A., Stempfhuber, B., Kirchhoff, F., Hirrlinger, J., Haslinger, A., Lie, D. C., et al. (2010) · Cell Stem Cell · doi:10.1016/j.stem.2010.11.017 · Cited by 335
Self-citationCiliary Hedgehog Signaling Restricts Injury-Induced Adipogenesis
Kopinke, D., Roberson, E. C., & Reiter, J. F. (2017) · Cell · doi:10.1016/j.cell.2017.06.035 · Cited by 260
Complex interactions between genes controlling trafficking in primary cilia
Ocbina, P. J. R., Eggenschwiler, J. T., Moskowitz, I., & Anderson, K. V. (2011) · Nature Genetics · doi:10.1038/ng.832 · Cited by 208
Lack of Primary Cilia Primes Shear-Induced Endothelial-to-Mesenchymal Transition
Egorova, A. D., Khedoe, P. P. S. J., Goumans, M.-J. T. H., Yoder, B. K., Nauli, S. M., ten Dijke, P., Poelmann, R. E., & Hierck, B. P. (2011) · Circulation Research · doi:10.1161/circresaha.110.231860 · Cited by 195
Self-citationRuiz-Perez, V. L., Blair, H. J., Rodriguez-Andres, M. E., Blanco, M. J., Wilson, A., Liu, Y.-N., Miles, C., Peters, H., & Goodship, J. A. (2007) · Development · doi:10.1242/dev.007542 · Cited by 175
Primary Cilia Are Not Required for Normal Canonical Wnt Signaling in the Mouse Embryo
Ocbina, P. J. R., Tuson, M., & Anderson, K. V. (2009) · PLoS ONE · doi:10.1371/journal.pone.0006839 · Cited by 164
How do cilia organize signalling cascades?
Nachury, M. V. (2014) · Philosophical Transactions of the Royal Society B: Biological Sciences · doi:10.1098/rstb.2013.0465 · Cited by 150
Yuan, X., Cao, J., He, X., Serra, R., Qu, J., Cao, X., & Yang, S. (2016) · Nature Communications · doi:10.1038/ncomms11024 · Cited by 122
Self-citationPrimary cilia sensitize endothelial cells to BMP and prevent excessive vascular regression
Vion, A.-C., Alt, S., Klaus-Bergmann, A., Szymborska, A., Zheng, T., Perovic, T., Hammoutene, A., Oliveira, M. B., Bartels-Klein, E., Hollfinger, I., et al. (2018) · Journal of Cell Biology · doi:10.1083/jcb.201706151 · Cited by 113
Guo, J., Otis, J. M., Suciu, S. K., Catalano, C., Xing, L., Constable, S., Wachten, D., Gupton, S., Lee, J., Lee, A., et al. (2019) · Developmental Cell · doi:10.1016/j.devcel.2019.11.005 · Cited by 100
Canonical and noncanonical intraflagellar transport regulates craniofacial skeletal development
Noda, K., Kitami, M., Kitami, K., Kaku, M., & Komatsu, Y. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1519458113 · Cited by 83
Hippocampal and Cortical Primary Cilia Are Required for Aversive Memory in Mice
Berbari, N. F., Malarkey, E. B., Yazdi, S. M. Z. R., McNair, A. D., Kippe, J. M., Croyle, M. J., Kraft, T. W., & Yoder, B. K. (2014) · PLoS ONE · doi:10.1371/journal.pone.0106576 · Cited by 69
Self-citationPrimary cilia maintain corneal epithelial homeostasis by regulation of the Notch signaling pathway
Grisanti, L., Revenkova, E., Gordon, R. E., & Iomini, C. (2016) · Development · doi:10.1242/dev.132704 · Cited by 71
Hwang, S.-H., White, K. A., Somatilaka, B. N., Shelton, J. M., Richardson, J. A., & Mukhopadhyay, S. (2018) · Development · doi:10.1242/dev.154054 · Cited by 60
Shimada, I. S., Hwang, S.-H., Somatilaka, B. N., Wang, X., Skowron, P., Kim, J., Kim, M., Shelton, J. M., Rajaram, V., Xuan, Z., et al. (2018) · Cell Reports · doi:10.1016/j.celrep.2018.01.018 · Cited by 52
Martin, L., Kaci, N., Estibals, V., Goudin, N., Garfa-Traore, M., Benoist-Lasselin, C., Dambroise, E., & Legeai-Mallet, L. (2018) · Human Molecular Genetics · doi:10.1093/hmg/ddx374 · Cited by 51
The Ciliary Protein Ftm Is Required for Ventricular Wall and Septal Development
Gerhardt, C., Lier, J. M., Kuschel, S., & Rüther, U. (2013) · PLoS ONE · doi:10.1371/journal.pone.0057545 · Cited by 42
McCafferty, C. L., Papoulas, O., Jordan, M. A., Hoogerbrugge, G., Nichols, C., Pigino, G., Taylor, D. W., Wallingford, J. B., & Marcotte, E. M. (2022) · eLife · doi:10.7554/elife.81977 · Cited by 40
Wang, L., Guo, Q., Acharya, S., Zheng, X., Huynh, V., Whitmore, B., Yimit, A., Malhotra, M., Chatterji, S., Rosin, N., et al. (2024) · Nature Neuroscience · doi:10.1038/s41593-024-01726-z · Cited by 33
Self-citationPrimary cilia are required for the persistence of memory and stabilization of perineuronal nets
Jovasevic, V., Zhang, H., Sananbenesi, F., Guedea, A. L., Soman, K. V., Wiktorowicz, J. E., Fischer, A., & Radulovic, J. (2021) · iScience · doi:10.1016/j.isci.2021.102617 · Cited by 32
TGF‐β Suppresses Ift88 Expression in Chondrocytic ATDC5 Cells
Kawasaki, M., Ezura, Y., Hayata, T., Notomi, T., Izu, Y., & Noda, M. (2015) · Journal of Cellular Physiology · doi:10.1002/jcp.25005 · Cited by 26
Mustafa, R., Kreiner, G., Kamińska, K., Wood, A.-E. J., Kirsch, J., Tucker, K. L., & Parlato, R. (2019) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2019.00565 · Cited by 18
Cilia function is associated with axon initial segment morphology
Wang, B., Hu, L., Sun, Z., & Zhang, Y. (2019) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2019.05.172 · Cited by 17
Cilia in cell signaling and human disorders
Duldulao, N. A., Li, J., & Sun, Z. (2010) · Protein & Cell · doi:10.1007/s13238-010-0098-7 · Cited by 13
The primary cilia are associated with the axon initial segment in neurons
Wang, H., Li, Y., Li, X., Sun, Z., Yu, F., Pashang, A., Kulasiri, D., Li, H. W., Chen, H., Hou, H., et al. (2025) · Advanced Science · doi:10.1002/advs.202407405 · Cited by 13
Temporal ablation of the ciliary protein IFT88 alters normal brainwave patterns
Strobel, M. R., Zhou, Y., Qiu, L., Hofer, A. M., & Chen, X. (2025) · Scientific Reports · doi:10.1038/s41598-024-83432-1 · Cited by 9
Astrocyte-specific inhibition of the primary cilium suppresses C3 expression in reactive astrocyte
Muhamad, N. A., Masutani, K., Furukawa, S., Yuri, S., Toriyama, M., Matsumoto, C., Itoh, S., Shinagawa, Y., Isotani, A., Toriyama, M., et al. (2024) · Cellular and Molecular Neurobiology · doi:10.1007/s10571-024-01482-5 · Cited by 9
Metadata
- Authors
- Haycraft, C. J., Zhang, Q., Song, B., Jackson, W. S., Detloff, P. J., Serra, R., & Yoder, B. K.
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 2007-01-15
- Volume
- 134
- Issue
- 2
- Pages
- 307-316
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W1968904141
- Cited by
- 408
- References
- 9
- FWCI
- 11.13
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
