The G protein-coupled receptor GPRC5B contributes to neurogenesis in the developing mouse neocortex
Abstract
Neural progenitor cells in the developing brain give rise to neurons and glia. Multiple extrinsic signalling molecules and their cognate membrane receptors have been identified to control neural progenitor fate. However, a role for G protein-coupled receptors in cell fate decisions in the brain remains largely putative. Here we show that GPRC5B, which encodes an orphan G protein-coupled receptor, is present in the ventricular surface of cortical progenitors in the mouse developing neocortex and is required for their neuronal differentiation. GPRC5B-depleted progenitors fail to adopt a neuronal fate and ultimately become astrocytes. Furthermore, GPRC5B-mediated signalling is associated with the proper regulation of β-catenin signalling, a pathway crucial for progenitor fate decision. Our study uncovers G protein-coupled receptor signalling in the neuronal fate determination of cortical progenitors.
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GPRC5B is a regulator of neurogenesis and inflammatory signaling in obesity.
“the latter being a regulator of neurogenesis72,73 and inflammatory signaling in obesity74.”
From Genetic meta-analysis of diagnosed Alzheimer’s disease identifies new risk loci and implicates Aβ, tau, immunity and lipid processing (2019) · cites this paper as [72]
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- Authors
- Kurabayashi, N., Nguyen, M. D., & Sanada, K.
- Affiliations
- Molecular Genetics Research Laboratory, Graduate School of Science, The University of Tokyo (JP)
- University of Calgary (CA)
- Corresponding author
- Sanada, Kamon
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 2013-11-01
- Volume
- 140
- Issue
- 21
- Pages
- 4335-4346
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W2049774426
- Cited by
- 46
- References
- 51
- Citations by year
- 2026: 1 · 2025: 7 · 2024: 2 · 2023: 1 · 2022: 7 · 2021: 4 · 2020: 4 · 2019: 5 · 2018: 3 · 2017: 2 · 2016: 3 · 2015: 1 · 2014: 6
- FWCI
- 1.24
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Development (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of Washington
- University of Tokyo
- University of Illinois at Urbana-Champaign
- Canadian Institutes of Health Research
- Japan Society for the Promotion of Science (24650161, 24700314)
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- GPRC5B, orphan G protein-coupled receptor, neurogenesis, neocortex development, cortical progenitor cells, neuronal differentiation, astrocyte differentiation, cell fate determination, β-catenin signaling, Wnt signaling, mouse brain development
- MeSH terms
- Animals, Cell Differentiation, Immunohistochemistry, Plasmids, Signal Transduction, In Situ Hybridization, DNA Primers, Electroporation, Gene Expression Regulation, Developmental, Neocortex, Receptors, G-Protein-Coupled, Mice, Neurogenesis, Neural Stem Cells
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