Cited by 1,157Neurogenesis and neuroplasticity mechanismsDevelopmental Biology and Gene RegulationNeuroscience and Neuropharmacology Research

Isolation of radial glial cells by fluorescent-activated cell sorting reveals a neuronal lineage

Malatesta, Paolo, Hartfuss, Eva, Götz, Magdalena·2000·Development

Abstract

The developing central nervous system of vertebrates contains an abundant cell type designated radial glial cells. These cells are known as guiding cables for migrating neurons, while their role as precursor cells is less clear. Since radial glial cells express a variety of astroglial characteristics and differentiate as astrocytes after completing their guidance function, they have been considered as part of the glial lineage. Using fluorescence-activated cell sorting, we show here that radial glial cells also are neuronal precursors and only later, after neurogenesis, do they shift towards an exclusive generation of astrocytes. These results thus demonstrate a novel function for radial glial cells, namely their ability to generate two major cell types found in the nervous system, neurons and astrocytes.

Integrity signals

Registry

Crossref · 10.1242/dev.127.24.5253

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • After symmetric divisions, radial glia divide asymmetrically, generating postmitotic excitatory neurons that migrate to their final location in the cerebral cortex by using the basal process of the radial glial cell as a scaffold.

    “After this period of symmetric divisions, these progenitor cells — also known as radial glia — divide asymmetrically, generating postmitotic excitatory neurons that will migrate to their final location in the cerebral cortex by using the basal process of the radial glial cell as a scaffold55–58.”

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Malatesta, P., Hartfuss, E., & Götz, M.
Affiliations
Max Planck Institute of Neurobiology (DE)
Corresponding author
Götz, Magdalena
Venue
Development
Published
2000-12-15
Volume
127
Issue
24
Pages
5253-5263
ISSN
  • 0950-1991 (ISSN-L)
  • 1477-9129
OpenAlex
W2168945089
Cited by
1,157
References
1
Citations by year
2026: 16 · 2025: 22 · 2024: 29 · 2023: 24 · 2022: 27 · 2021: 28 · 2020: 47 · 2019: 38 · 2018: 29 · 2017: 29 · 2016: 43 · 2015: 42 · 2014: 56 · 2013: 58 · 2012: 49
FWCI
12.08
Type
journal article
Language
en
Open access
closed
Locations
  • Development (publishedVersion)
  • PubMed (publishedVersion)
  • CINECA IRIS Institutial Research Information System (University of Genoa) (submittedVersion)
Topics
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords
radial glia, neuronal lineage, neuronal precursors, astrocyte differentiation, glial lineage, neurogenesis, central nervous system development
MeSH terms
Animals, Astrocytes, Cell Differentiation, Cell Separation, Cerebral Cortex, Flow Cytometry, Glial Fibrillary Acidic Protein, Luminescent Proteins, Male, Mice, Inbred C57BL, Mice, Transgenic, Neuroglia, Neurons, Promoter Regions, Genetic, Recombinant Proteins, Stem Cells, Rats, Wistar, ATP-Binding Cassette Transporters, Amino Acid Transport System X-AG, Green Fluorescent Proteins, Mice, Rats

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review