Cleavage orientation and the asymmetric inheritance of notchl immunoreactivity in mammalian neurogenesis
Open accessAbstract
Neurons in the mammalian central nervous system are generated from progenitor cells near the lumen of the neural tube. Time-lapse microscopy of dividing cells in slices of developing cerebral cortex reveals that cleavage orientation predicts the fates of daughter cells. Vertical cleavages produce behaviorally and morphologically identical daughters that resemble precursor cells; these symmetric divisions may serve to expand or maintain the progenitor pool. In contrast, horizontally dividing cells produce basal daughters that behave like young migratory neurons and apical daughters that remain within the proliferative zone. Notch1 immunoreactivity is distributed asymmetrically in mitotic cells, with Notch1 inherited selectively by the basal (neuronal) daughter of horizontal divisions. These results provide cellular and molecular evidence that cortical neurons are generated from asymmetric divisions.
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During brain development, RGCs can divide symmetrically or asymmetrically; symmetric divisions result in two daughter RGCs, while asymmetric divisions produce a RGC and one other cell.
“During brain development, RGCs can divide symmetrically or asymmetrically. While symmetric divisions result in two daughter RGCs, asymmetric divisions produce a RGC and one another cell [96].”
From The ASD Living Biology: from cell proliferation to clinical phenotype (2019) · cites this paper as [96]
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- Authors
- Chenn, A., & McConnell, S. K.
- Affiliations
- Stanford University (US)
- ORCID
- McConnell, Susan K.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1995-08
- Volume
- 82
- Issue
- 4
- Pages
- 631-641
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2021515035
- Cited by
- 766
- References
- 44
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 11 · 2023: 18 · 2022: 12 · 2021: 12 · 2020: 21 · 2019: 15 · 2018: 15 · 2017: 18 · 2016: 20 · 2015: 16 · 2014: 23 · 2013: 28 · 2012: 22
- FWCI
- 10.07
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Cell (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Funders
- National Institute of Mental Health (MH51864)
- National Institute of General Medical Sciences (T32GM007365, GM 07365)
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Congenital heart defects research
- Keywords
- cleavage orientation, asymmetric cell division, Notch1, neurogenesis, cortical development, neural progenitor cells, neuronal fate specification, time-lapse microscopy
- MeSH terms
- Animals, Cell Division, Cerebral Cortex, Ferrets, Immunohistochemistry, Membrane Proteins, Neurons, Receptors, Cell Surface, Stem Cells, Time Factors, Transcription Factors, Cell Polarity, Receptor, Notch1
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