Expression of Cnox-2, a HOM/HOX homeobox gene in hydra, is correlated with axial pattern formation
Abstract
Cnox-2 is a HOM/HOX homeobox gene that we have identified in the simple metazoan Hydra vulgaris (Cnidaria: Hydrozoa). Cnox-2 is most closely related to anterior members of the Antennapedia gene complex from Drosophila, with the greatest similarity to Deformed. The Cnox-2 protein is expressed in the epithelial cells of adult hydra polyps in a region-specific pattern along the body axis, at a low level in the head and at a high level in the body column and the foot. The expression pattern of Cnox-2 is consistent with a role in axial pattern formation. Alteration of hydra axial patterning by treatment with diacylglycerol (DAG) results in an increase of head activation down the body column and in a coordinate reduction of Cnox-2 expression in epithelial cells in 'head-like' regions. These results suggest that Cnox-2 expression is negatively regulated by a signaling pathway acting through protein kinase C (PKC), and that the varying levels of expression of Cnox-2 along the body axis have the potential to result in differential gene expression which is important for hydra pattern formation.
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Cnidarian Hox-like genes are involved in patterning the oral-aboral axis during regeneration or asexual reproduction of the polyp stage.
“It has been shown that cnidarian Hox-like genes are involved in the patterning of the oral-aboral axis during regeneration or asexual reproduction of the polyp stage (Schummer et al., 1992;Shenk et al., 1993;Cartwright and Buss, 1999;Galliot 2000).”
From Conservation of Hox/ParaHox-Related Genes in the Early Development of a Cnidarian (2001) · cites this paper as Shenk et al., 1993
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- Authors
- Shenk, M. A., Bode, H. R., & Steele, R. E.
- Affiliations
- University of California, Irvine (US)
- Corresponding author
- Shenk, M. Andrew
- ORCID
- Steele, Robert E.
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 1993-02-01
- Volume
- 117
- Issue
- 2
- Pages
- 657-667
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W2341734344
- Cited by
- 129
- References
- 59
- Citations by year
- 2020: 1 · 2019: 2 · 2017: 3 · 2015: 2 · 2013: 1 · 2012: 1
- FWCI
- 4.12
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Development (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5p01hd027173-05, 5r01hd024511-04)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD 24511, HD-07029, HD 27173)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Physical Sciences › Earth and Planetary Sciences › Paleontology › Marine Invertebrate Physiology and Ecology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- Hydra vulgaris, body axis patterning, Deformed, diacylglycerol, protein kinase C signaling, epithelial cells, gene expression regulation
- MeSH terms
- Amino Acid Sequence, Animals, Base Sequence, Diglycerides, Drosophila melanogaster, Genes, Homeobox, Hydra, Microscopy, Fluorescence, Molecular Sequence Data, Morphogenesis, Gene Expression, Sequence Homology, Amino Acid
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