The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
The Drosophila disembodied gene controls late embryonic morphogenesis and codes for a cytochrome P450 enzyme that regulates embryonic ecdysone levels
Abstract
Ecdysteroids regulate a wide variety of cellular processes during arthropod development, yet little is known about the genes involved in the biosynthesis of these hormones. Previous studies have suggested that production of 20-hydroxyecdysone in Drosophila and other arthropods involves a series of cytochrome P450 catalyzed hydroxylations of cholesterol. In this report, we show that the disembodied (dib) locus of Drosophila codes for a P450-like sequence. In addition, we find that dib mutant embryos have very low titers of ecdysone and 20-hydroxyecdysone (20E) and fail to express IMP-E1 and L1, two 20E-inducible genes, in certain tissues of the embryo. In situ hybridization studies reveal that dib is expressed in a complex pattern in the early embryo, which eventually gives way to restricted expression in the prothoracic portion of the ring gland. In larval and adult tissues, dib expression is observed in the prothoracic gland and follicle cells of the ovaries respectively, two tissues known to synthesize ecdysteroids. Phenotypic analysis reveals that dib mutant embryos produce little or no cuticle and exhibit severe defects in many late morphogenetic processes such as head involution, dorsal closure and gut development. In addition, we examined the phenotypes of several other mutants that produce defective embryonic cuticles. Like dib, mutations in the spook (spo) locus result in low embryonic ecdysteroid titers, severe late embryonic morphological defects, and a failure to induce IMP-E1. From these data, we conclude that dib and spo likely code for essential components in the ecdysone biosynthetic pathway and that ecdysteroids regulate many late embryonic morphogenetic processes such as cell movement and cuticle deposition.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Temporal control of puffing activity in polytene chromosomes
Ashburner, M., Chihara, C., Meltzer, P., & Richards, G. (1974) · Cold Spring Harbor Symposia on Quantitative Biology · doi:10.1101/sqb.1974.038.01.070 · Cited by 641
The dare gene: steroid hormone production, olfactory behavior, and neural degeneration in Drosophila
Freeman, M. R., Dobritsa, A., Gaines, P., Segraves, W. A., & Carlson, J. R. (1999) · Development · doi:10.1242/dev.126.20.4591 · Cited by 119
Cited by
Insect CYP Genes and P450 Enzymes
Feyereisen, R. (2012) · Insect Molecular Biology and Biochemistry · doi:10.1016/b978-0-12-384747-8.10008-x · Cited by 566
Control and biochemical nature of the ecdysteroidogenic pathway
Gilbert, L. I., Rybczynski, R., & Warren, J. T. (2002) · Annual Review of Entomology · doi:10.1146/annurev.ento.47.091201.145302 · Cited by 532
Prothoracicotropic Hormone Regulates Developmental Timing and Body Size in Drosophila
McBrayer, Z., Ono, H., Shimell, M., Parvy, J.-P., Beckstead, R. B., Warren, J. T., Thummel, C. S., Dauphin-Villemant, C., Gilbert, L. I., & O'Connor, M. B. (2007) · Developmental Cell · doi:10.1016/j.devcel.2007.11.003 · Cited by 434
Self-citationFeyereisen, R. (2005) · Comprehensive Molecular Insect Science · doi:10.1016/b0-44-451924-6/00049-1 · Cited by 420
Characterization of Drosophila melanogaster cytochrome P450 genes
Chung, H., Sztal, T., Pasricha, S., Sridhar, M., Batterham, P., & Daborn, P. J. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0812141106 · Cited by 310
Molecular Mechanisms of Developmental Timing in C. elegans and Drosophila
Thummel, C. S. (2001) · Developmental Cell · doi:10.1016/s1534-5807(01)00060-0 · Cited by 310
Gilbert, L. I. (2004) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2003.11.003 · Cited by 300
Warren, J. T., Petryk, A., Marqués, G., Parvy, J.-P., Shinoda, T., Itoyama, K., Kobayashi, J., Jarcho, M., Li, Y., O’Connor, M. B., et al. (2004) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2004.06.009 · Cited by 296
Self-citationChoudhary, D., Jansson, I., Stoilov, I., Sarfarazi, M., & Schenkman, J. B. (2005) · Archives of Biochemistry and Biophysics · doi:10.1016/j.abb.2005.02.001 · Cited by 261
Enzymes for ecdysteroid biosynthesis: Their biological functions in insects and beyond
Niwa, R., & Niwa, Y. S. (2014) · Bioscience, Biotechnology, and Biochemistry · doi:10.1080/09168451.2014.942250 · Cited by 249
Steroid signaling in plants and insects—common themes, different pathways
Thummel, C. S., & Chory, J. (2002) · Genes & Development · doi:10.1101/gad.1042102 · Cited by 161
Vesicle-Mediated Steroid Hormone Secretion in Drosophila melanogaster
Yamanaka, N., Marqués, G., & O’Connor, M. B. (2015) · Cell · doi:10.1016/j.cell.2015.10.022 · Cited by 147
Self-citationRegulation of ecdysone production in Drosophila by neuropeptides and peptide hormones
Kannangara, J. R., Mirth, C. K., & Warr, C. G. (2021) · Open Biology · doi:10.1098/rsob.200373 · Cited by 84
Tsukagoshi, Y., Ohyama, K., Seki, H., Akashi, T., Muranaka, T., Suzuki, H., & Fujimoto, Y. (2016) · Phytochemistry · doi:10.1016/j.phytochem.2016.03.010 · Cited by 27
Metadata
- Authors
- Chávez, V. M., Marqués, G., Delbecque, J. P., Kobayashi, K., Hollingsworth, M., Burr, J., Natzle, J. E., & O’Connor, M. B.
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 2000-10-01
- Volume
- 127
- Issue
- 19
- Pages
- 4115-4126
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- OpenAlex
- W2109286448
- Cited by
- 297
- References
- 2
- FWCI
- 3.43
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
