Temporal control of puffing activity in polytene chromosomes

Ashburner, M.; Chihara, C.; Meltzer, P.; Richards, G.

doi:10.1101/sqb.1974.038.01.070

PreprintCited by 643Genomics and Chromatin DynamicsChromosomal and Genetic VariationsDevelopmental Biology and Gene Regulation

Temporal control of puffing activity in polytene chromosomes

Ashburner, M., Chihara, C., Meltzer, P., Richards, G.·1974·Cold Spring Harbor Symposia on Quantitative Biology

DOI 10.1101/sqb.1974.038.01.070

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Abstract

The genetic organization of individual bands of polytene chromosomes is an enigma. On the one hand, we have evidence (Rudkin, 1965; Daneholt and Edström, 1967; Mulder et al, 1968; see also Rasch et al., 1971) that an “average” band contains DNA equivalent to 40,000 or so nucleotide pairs. The range is perhaps between 3000 and 100,000 nucleotide pairs (Beermann, 1972). On the other hand, classical (e.g., Painter, 1934; Bridges, 1935, 1936; Mackensen, 1935; Muller and Prokofyeva, 1935) and more modern (e.g., Hochman, 1971; Lefevre, 1971; Lim et al., 1971; Judd et al., 1972; Rayle, 1972) evidence strongly suggests that one band (or band plus adjacent interband(s), if you will: Crick, 1971) corresponds to a unit of genetic function.

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Crossref · 10.1101/sqb.1974.038.01.070

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The polytene chromosome model allowed description of the induction by 20E of a specific sequence of puffs, a key finding for understanding the mechanism of action of steroid hormones through their binding to nuclear receptors and inducing a sequence of gene activations.
“This biological model allowed description of the induction by 20E of a specific sequence of puffs, and this was a key finding for understanding the mechanism of action of steroid hormones through their binding to nuclear receptors and inducing a sequence of gene activations (Figure 5) [15,16].”
From Ecdysteroids (2021) · cites this paper as [16]

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Background
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“According to the Ashburner model (Ashburner et al., 1974), postlarval development begins by the hormone-dependent activation of a small set of “early” genes that include Broad-Complex (BR-C), E74, and E75.”
Background
Ecdysone coordinates the timing and amounts of E74A and E74B transcription in Drosophila.Preprint
Karim, F. D., & Thummel, C. S. (1991) · Genes & Development · doi:10.1101/gad.5.6.1067 · Cited by 145 · see citing works
Inducible expression of double-stranded RNA directs specific genetic interference in Drosophila
Lam, G., & Thummel, C. S. (2000) · Current Biology · doi:10.1016/s0960-9822(00)00631-x · Cited by 142 · see citing works
Glucocorticoid regulation of adipocyte differentiation: hormonal triggering of the developmental program and induction of a differentiation-dependent gene.
Chapman, A. B., Knight, D. M., & RINGOLD, G. M. (1985) · The Journal of Cell Biology · doi:10.1083/jcb.101.4.1227 · Cited by 141 · see citing works
Peptide Hormones, Steroid Hormones, and Puffs: Mechanisms and Models in Insect Development
Henrich, V. C., Rybczynski, R., & Gilbert, L. I. (1998) · Vitamins and hormones · doi:10.1016/s0083-6729(08)60934-6 · Cited by 141 · see citing works
Cytogenetic analysis of the 2B3-4-2B11 Region of the X-chromosome of Drosophila melanogaster
Belyaeva, E. S., Aizenzon, M. G., Semeshin, V. F., Kiss, I., Koczka, K., Baritcheva, E. M., Gorelova, T. D., & Zhimulev, I. F. (1980) · Chromosoma · doi:10.1007/bf00285954 · Cited by 139 · see citing works
20-Hydroxyecdysone is required for, and negatively regulates, transcription of Drosophila pupal cuticle protein genes
Apple, R. T., & Fristrom, J. W. (1991) · Developmental Biology · doi:10.1016/0012-1606(91)90257-4 · Cited by 140 · see citing works
Rapid Gene Regulation by Auxin
Theologis, A. (1986) · Annual Review of Plant Physiology · doi:10.1146/annurev.pp.37.060186.002203 · Cited by 139 · see citing works
Thyroid Hormone-dependent Gene Expression Program for Xenopus Neural Development
Denver, R. J., Pavgi, S., & Shi, Y. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.13.8179 · Cited by 136 · see citing works
Puffs and PCR: the in vivo dynamics of early gene expression during ecdysone responses in Drosophila
Huet, F., Ruiz, C., & Richards, G. (1993) · Development · doi:10.1242/dev.118.2.613 · Cited by 135 · see citing works
Self-citation
Sequential gene activation by ecdysone in Drosophila melanogaster: the hierarchical equivalence of early and early late genes
Huet, F., Ruiz, C., & Richards, G. (1995) · Development · doi:10.1242/dev.121.4.1195 · Cited by 134 · see citing works
Self-citation
The Drosophila E74 gene is required for metamorphosis and plays a role in the polytene chromosome puffing response to ecdysone
Fletcher, J. C., Burtis, K. C., Hogness, D. S., & Thummel, C. S. (1995) · Development · doi:10.1242/dev.121.5.1455 · Cited by 133 · see citing works
Insect Growth- and Development-Disrupting Insecticides
Dhadialla, T. S., Retnakaran, A., & Smagghe, G. J. (2005) · Elsevier eBooks · doi:10.1016/b0-44-451924-6/00076-4 · Cited by 139 · see citing works
The RXR ortholog USP suppresses early metamorphic processes in Drosophila in the absence of ecdysteroids
Schubiger, M., & Truman, J. W. (2000) · Development · doi:10.1242/dev.127.6.1151 · Cited by 129 · see citing works
The Ecdysone-Induced DHR4 Orphan Nuclear Receptor Coordinates Growth and Maturation in Drosophila
King‐Jones, K., Charles, J., Lam, G., et al. (2005) · Cell · doi:10.1016/j.cell.2005.03.030 · Cited by 129 · see citing works
“As a further test of a role for DHR4 in repressing early gene expression, we ectopically expressed DHR4 in late L3 at the time when EcR and the classic early puff transcripts, E74A and E75A, are initially being induced by ecdysone (Ashburner et al., 1974) (Figure 4B).”
Mosquito ecdysteroid receptor: Analysis of the cDNA and expression during vitellogenesis
Cho, W.-L., Kapitskaya, M. Z., & Raikhel, A. S. (1995) · Insect Biochemistry and Molecular Biology · doi:10.1016/0965-1748(94)00045-j · Cited by 126 · see citing works
Coordinate regulation of small temporal RNAs at the onset of Drosophila metamorphosis
Bashirullah, A., Pasquinelli, A. E., Kiger, A. A., Perrimon, N., Ruvkun, G., & Thummel, C. S. (2003) · Developmental Biology · doi:10.1016/s0012-1606(03)00063-0 · Cited by 126 · see citing works
“This hormone–receptor complex directly induces the expression of primary-response target genes, at least some of which encode transcription factors that transduce and amplify the hormonal signal by coordinating the expression of large batteries of downstream secondary-response late genes (Ashburner et al., 1974; Thumm…”
Background
Neuroendocrine Regulation of Ecdysis
Žitňan, D., & Adams, M. E. (2011) · Elsevier eBooks · doi:10.1016/b978-0-12-384749-2.10007-x · Cited by 127 · see citing works
Sequential gene activation by ecdysone in polytene chromosomes of Drosophila melanogaster,
Ashburner, M., & Richards, G. (1976) · Developmental Biology · doi:10.1016/0012-1606(76)90302-x · Cited by 127 · see citing works
Self-citation

Dissection of Larval Salivary Glands and Polytene Chromosome Preparation: Figure 1.Preprint
Kennison, J. A. (2008) · Cold Spring Harbor Protocols · doi:10.1101/pdb.prot4708 · Cited by 14 · see citing works
Autoradiography of Polytene Chromosomes of Rhynchosciara angelae at Different Stages of Larval Development
Ficq, A., & Pavan, C. (1957) · Nature · doi:10.1038/180983a0 · Cited by 174 · see citing works
Using PRINS for gene mapping in polytene chromosomes
Gu, H. F., Lind, M., Wieslander, L., et al. (1997) · Chromosome Research · doi:10.1023/a:1018417013699 · Cited by 11 · see citing works
Sequence diversity of polytene chromosome DNA from Drosophila hydei
Dickson, E., Boyd, J. B., & Laird, C. D. (1971) · Journal of Molecular Biology · doi:10.1016/0022-2836(71)90067-2 · Cited by 128 · see citing works
Polytene Chromosomes in Mammalian Cells
Zybina, E. V., & Zybina, T. G. (1996) · International review of cytology · doi:10.1016/s0074-7696(08)62220-2 · Cited by 157 · see citing works

Authors: Ashburner, M., Chihara, C., Meltzer, P., & Richards, G.
Affiliations: University of Cambridge (GB)
ORCID: Ashburner, M.
Venue: Cold Spring Harbor Symposia on Quantitative Biology
Publisher: Cold Spring Harbor Laboratory
Published: 1974-01-01
Volume: 38
Issue: 0
Pages: 655-662
ISSN: 0091-7451 (ISSN-L)
1943-4456
DOI: 10.1101/sqb.1974.038.01.070
OpenAlex: W2136267625
Cited by: 641
References: 0
Citations by year: 2026: 6 · 2025: 7 · 2024: 10 · 2023: 7 · 2022: 4 · 2021: 7 · 2020: 9 · 2019: 6 · 2018: 5 · 2017: 9 · 2016: 9 · 2015: 5 · 2014: 12 · 2013: 11 · 2012: 14
Type: posted content
Language: en
Open access: closed
Locations: Cold Spring Harbor Symposia on Quantitative Biology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords: polytene chromosomes, puffing, chromosome banding, genetic organization, interbands, Drosophila
MeSH terms: Animals, Anti-Bacterial Agents, Chromosomes, Cycloheximide, DNA, Drosophila melanogaster, Ecdysone, Enzyme Repression, Genes, Puromycin, RNA, Time Factors, Transcription, Genetic, Protein Biosynthesis

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Temporal control of puffing activity in polytene chromosomes

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