Cited by 133Alzheimer's disease research and treatmentsMicrotubule and mitosis dynamicsProtein Kinase Regulation and GTPase Signaling

Evidence that phosphorylation of the microtubule-associated protein Tau by SAPK4/p38δ at Thr50 promotes microtubule assembly

Feijoo, Carmen, Campbell, David G., Jakes, Ross, Goedert, Michel, Cuenda, Ana·2005·Journal of Cell Science

Abstract

Phosphorylation regulates both normal and pathological Tau functioning. This microtubule-associated protein plays a role in the organization and integrity of the neuronal cytoskeleton under normal conditions and becomes hyperphosphorylated and aggregated in a number of neurodegenerative diseases referred to as tauopathies. In this study, we identify and compare the residues in human Tau phosphorylated in vitro by all four p38 MAPK isoforms, and study the regulation of the phosphorylation of Thr50, under conditions where p38 MAPKs are active in cells. Through biochemical analysis, loss of function studies and analysis of endogenous and overexpressed Tau proteins, we show that SAPK4/p38delta is the major kinase phosphorylating Thr50 in Tau, when cells are exposed to osmotic stress. We also show that mutation of Thr50 to glutamic acid, which mimics phosphorylation, increases the ability of Tau to promote tubulin polymerisation in vitro and in vivo. Moreover, we show that Thr50 is phosphorylated in filamentous Tau from Alzheimer's disease brain. These findings suggest a role for Tau in the adaptative response of neurons to stress and indicate that SAPK4/p38delta and/or SAPK3/p38delta may contribute to the hyperphosphorylation of Tau in the human tauopathies.

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Crossref · 10.1242/jcs.01655

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Authors
Feijoo, C., Campbell, D. G., Jakes, R., Goedert, M., & Cuenda, A.
Affiliations
  • MRC Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dundee, DD1 5EH, UK
  • University of Dundee (GB)
  • MRC Laboratory of Molecular Biology (GB)
Corresponding author
Cuenda, Ana
Venue
Journal of Cell Science
Published
2005-01-15
Volume
118
Issue
2
Pages
397-408
ISSN
  • 0021-9533 (ISSN-L)
  • 1477-9137
OpenAlex
W2108675351
Cited by
133
References
65
Citations by year
2026: 6 · 2025: 5 · 2024: 2 · 2023: 4 · 2022: 7 · 2021: 6 · 2020: 7 · 2019: 7 · 2018: 3 · 2017: 7 · 2016: 8 · 2015: 5 · 2014: 5 · 2013: 7 · 2012: 8
FWCI
4.61
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Cell Science (publishedVersion)
  • PubMed (publishedVersion)
  • HighWire Press Open Archive (submittedVersion)
Topics
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
Keywords
tau protein, microtubule assembly, tau hyperphosphorylation, tauopathy, Alzheimer's disease, osmotic stress, neuronal stress response, neuronal cytoskeleton
MeSH terms
Cell Line, Humans, Microtubules, Phosphorylation, Threonine, tau Proteins, Protein Isoforms, p38 Mitogen-Activated Protein Kinases, Mitogen-Activated Protein Kinase 13

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