Cited by 610Alzheimer's disease research and treatmentsDementia and Cognitive Impairment ResearchNeuroinflammation and Neurodegeneration Mechanisms

Monoclonal antibody AT8 recognises tau protein phosphorylated at both serine 202 and threonine 205

Goedert, M., Jakes, R., Vanmechelen, E.·1995·Neuroscience Letters

Abstract

Hyperphosphorylated microtubule-associated protein tau is the major component of the paired helical filament of Alzheimer's disease. Phosphorylation-dependent anti-tau antibodies are being used to identify specific amino acids that are phosphorylated in tau from normal brain and Alzheimer's disease brain. As such, monoclonal antibody AT8 is widely used. By a combination of site-directed mutagenesis of recombinant tau and in vitro phosphorylation, we show that AT8 requires tau protein to be phosphorylated at both serine 202 and threonine 205 (using the numbering of the longest human brain tau isoform.

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Crossref · 10.1016/0304-3940(95)11484-e

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23 of 23 references verified0 citation errors · 0 not checkable

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Authors
Goedert, M., Jakes, R., & Vanmechelen, E.
Affiliations
  • MRC Laboratory of Molecular Biology (GB)
  • Fujirebio (Belgium) (BE)
Corresponding author
Goedert, M.
Venue
Neuroscience Letters
Publisher
Elsevier BV
Published
1995-04
Volume
189
Issue
3
Pages
167-170
ISSN
0304-3940 (ISSN-L)
OpenAlex
W2062772621
Cited by
608
References
24
Citations by year
2026: 36 · 2025: 33 · 2024: 20 · 2023: 30 · 2022: 27 · 2021: 26 · 2020: 20 · 2019: 20 · 2018: 20 · 2017: 15 · 2016: 12 · 2015: 22 · 2014: 12 · 2013: 17 · 2012: 14
FWCI
11.08
Type
journal article
Language
en
Open access
closed
Locations
  • Neuroscience Letters (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
  • Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
Keywords
tau protein, tau phosphorylation, paired helical filaments, Alzheimer's disease, anti-tau antibodies, site-directed mutagenesis, in vitro phosphorylation
MeSH terms
Amino Acid Sequence, Antibodies, Monoclonal, Cloning, Molecular, Escherichia coli, Humans, Molecular Sequence Data, Phosphorylation, Protein Kinases, Recombinant Proteins, Serine, Threonine, tau Proteins, DNA, Complementary

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