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Cited by 77

The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.

Dissecting the role of PtdIns(4,5)P 2 in endocytosis and recycling of the transferrin receptor

Abe, Namiko, Inoue, Takanari, Galvez, Thierry, Klein, Lawrence, Meyer, Tobias·2008·Journal of Cell Science
DOI 10.1242/jcs.020792
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Abstract

Endocytosis and recycling of membrane proteins are key processes for nutrient uptake, receptor signaling and synaptic transmission. Different steps in these fission and fusion cycles have been proposed to be regulated by physiological changes in plasma membrane (PM) phosphatidylinositol (4,5)-bisphosphate [PtdIns(4,5)P2] concentration. Here, we use a chemical enzyme-translocation strategy to rapidly reduce PM PtdIns(4,5)P2 levels while monitoring clathrin-mediated endocytosis and recycling. PtdIns(4,5)P2 hydrolysis blocked transferrin receptor endocytosis and led to a marked increase in the concentration of transferrin receptors in the PM, suggesting that endocytosis is more sensitive to changes in PtdIns(4,5)P2 than recycling. Reduction of PM PtdIns(4,5)P2 levels led to a near complete dissociation of Adaptor protein 2 (AP-2) from the PM but had only a small effect on clathrin assembly. This argues that receptor-mediated PtdIns(4,5)P2 reduction preferentially suppresses AP-2-mediated targeting of cargo to endocytic sites rather than the assembly of clathrin coats or recycling of endocytic vesicles.

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Research works printed but not linked: 23

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Metadata

Authors
Abe, N., Inoue, T., Galvez, T., Klein, L., & Meyer, T.
Affiliations
  • Graduate Program in Neurosciences, Stanford University School of Medicine, Stanford, CA 94305, USA
  • Department of Chemical and Systems Biology, Stanford University School of Medicine, Clark Center, 318 Campus Drive, Stanford, CA 94305, USA
  • Graduate Program in Biophysics, and Department of Microbiology and Immunology, Beckman Center, 279 Campus Drive, Stanford University Medical School, Stanford, CA 94305, USA
Venue
Journal of Cell Science
Publisher
The Company of Biologists
Published
2008-05-01
Volume
121
Issue
9
Pages
1488-1494
ISSN
  • 1477-9137
  • 0021-9533
DOI
10.1242/jcs.020792
Cited by
77
References
4
Language
en

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CoChat. (2026). Citation check: Dissecting the role of PtdIns(4,5)P 2 in endocytosis and recycling of the transferrin receptor. https://app.cochat.ai/papers/10.1242/jcs.020792
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Abe, N., Inoue, T., Galvez, T., Klein, L., & Meyer, T. (2008). Dissecting the role of PtdIns(4,5)P 2 in endocytosis and recycling of the transferrin receptor. Journal of Cell Science. https://doi.org/10.1242/jcs.020792

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