Pax7 and myogenic progression in skeletal muscle satellite cells
Abstract
Skeletal muscle growth and regeneration are attributed to satellite cells - muscle stem cells resident beneath the basal lamina that surrounds each myofibre. Quiescent satellite cells express the transcription factor Pax7 and when activated, coexpress Pax7 with MyoD. Most then proliferate, downregulate Pax7 and differentiate. By contrast, others maintain Pax7 but lose MyoD and return to a state resembling quiescence. Here we show that Pax7 is able to drive transcription in quiescent and activated satellite cells, and continues to do so in those cells that subsequently cease proliferation and withdraw from immediate differentiation. We found that constitutive expression of Pax7 in satellite-cell-derived myoblasts did not affect MyoD expression or proliferation. Although maintained expression of Pax7 delayed the onset of myogenin expression it did not prevent, and was compatible with, myogenic differentiation. Constitutive Pax7 expression in a Pax7-null C2C12 subclone increased the proportion of cells expressing MyoD, showing that Pax7 can act genetically upstream of MyoD. However these Pax7-null cells were unable to differentiate into normal myotubes in the presence of Pax7. Therefore Pax7 may be involved in maintaining proliferation and preventing precocious differentiation, but does not promote quiescence.
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Pax7+ cells might be more proliferative and less committed than their Pax7− counterparts.
“These results are consistent with the idea that Pax7+ cells might be more proliferative (Zammit et al., 2006) and less committed than their Pax7− counterparts.”
From Aging induces aberrant state transition kinetics in murine muscle stem cells (2020) · cites this paper as Zammit et al., 2006
Pax7 promotes proliferation in certain contexts.
“Previous functional studies with Pax7 overexpression constructs in MuSCs reported that Pax7 promotes proliferation in certain contexts (Zammit et al., 2006), consistent with increased Pax7 as cells enter into cycle later in the activation process.”
From Aging induces aberrant state transition kinetics in murine muscle stem cells (2020) · cites this paper as Zammit et al., 2006
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- Authors
- Zammit, P. S., Relaix, F., Nagata, Y., Ruiz, A. P., Collins, C. A., Partridge, T. A., & Beauchamp, J. R.
- Affiliations
- King's College London (GB)
- CNRS URA 2578, Département de Biologie Du Développement, Institut Pasteur, 25 Rue du Dr Roux, 75724 Paris CEDEX 15, France
- Hammersmith Hospital (GB)
- Muscle Cell Biology Group, Medical Research Council Clinical Sciences Centre, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London, W12 0NN, UK
- Corresponding author
- Zammit, Peter S.
- Venue
- Journal of Cell Science
- Publisher
- The Company of Biologists
- Published
- 2006-05-01
- Volume
- 119
- Issue
- 9
- Pages
- 1824-1832
- ISSN
- 0021-9533 (ISSN-L)
- 1477-9137
- OpenAlex
- W2096622475
- Cited by
- 614
- References
- 46
- Citations by year
- 2026: 26 · 2025: 30 · 2024: 27 · 2023: 35 · 2022: 35 · 2021: 27 · 2020: 28 · 2019: 36 · 2018: 27 · 2017: 34 · 2016: 32 · 2015: 53 · 2014: 30 · 2013: 41 · 2012: 27
- FWCI
- 9.51
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Cell Science (publishedVersion)
- PubMed (publishedVersion)
- Research Portal (King's College London) (submittedVersion)
- HighWire Press Open Archive (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Congenital heart defects research
- Keywords
- Pax7, skeletal muscle satellite cells, myogenesis, MyoD, quiescence, myoblasts, myotube formation, transcriptional regulation, muscle regeneration, C2C12 myoblasts
- MeSH terms
- Animals, Cell Differentiation, Cell Fusion, Cells, Cultured, Gene Expression Regulation, Recombinant Fusion Proteins, Transcription, Genetic, MyoD Protein, Muscle, Skeletal, Satellite Cells, Skeletal Muscle, Cell Proliferation, Mice, PAX7 Transcription Factor
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