CHUP1 mediates actin-based light-induced chloroplast avoidance movement in the moss Physcomitrella patens
Abstract
Chloroplasts change their intracellular distribution in response to light intensity. CHUP1 (CHLOROPLAST UNUSUAL POSITIONING1) is indispensable for this response in Arabidopsis thaliana. However, involvement of CHUP1 in light-induced chloroplast movement is unknown in other plants. In this study, CHUP1 orthologues were isolated from a moss, Physcomitrella patens, and a fern, Adiantum capillus-veneris, by cDNA library screening and PCR cloning based on the P. patens genome sequence. Functional motifs found in CHUP1 of A. thaliana were conserved among the CHUP1 orthologues. In addition to the putative functional regions, the C-terminal regions (approximately 250 amino acids), which are unique in CHUP1s, were highly conserved. Green fluorescent protein (GFP) fusions of P. patens CHUP1s (PpCHUP1A, PpCHUP1B and PpCHUP1C) were transiently expressed in protoplast cells. All GFP fusions were localized on the chloroplasts. Light-induced chloroplast avoidance movement of chup1 disruptants of P. patens was examined in the presence of cytoskeletal inhibitors because of the utilization of both microtubules and actin filaments for the movement in P. patens. When actin filaments were disrupted by cytochalasin B, the wild type (WT) and all chup1 disruptants showed chloroplast avoidance movement. However, when microtubules were disrupted by Oryzalin, chloroplasts in ∆chup1A and ∆chup1A/B rarely moved and stayed in the strong light-irradiated area. On the other hand, WT, ∆chup1B and ∆chup1C showed chloroplast avoidance movement. These results suggest that PpCHUP1A predominantly mediates the actin-based light-induced chloroplast avoidance movement. This study reveals that CHUP1 functions on the chloroplasts and is involved in the actin-based light-induced chloroplast avoidance movement in P. patens.
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CHUP1 is solely involved in actin-mediated motility but not in microtubule-mediated motility.
“CHUP1 is solely involved in actin-mediated motility but not in microtubule-mediated motility”
From The action of enhancing weak light capture via phototropic growth and chloroplast movement in plants (2022) · cites this paper as Usami et al., 2012
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- Authors
- Usami, H., Maeda, T., Fujii, Y., Oikawa, K., Takahashi, F., Kagawa, T., Wada, M., & Kasahara, M.
- Affiliations
- Ritsumeikan University (JP)
- National Institute for Basic Biology (JP)
- Japan Science and Technology Agency (JP)
- Institute of Agrobiological Sciences (JP)
- Kyushu University (JP)
- Corresponding author
- Kasahara, Masahiro
- Venue
- Planta
- Published
- 2012-08-30
- Volume
- 236
- Issue
- 6
- Pages
- 1889-1897
- ISSN
- 0032-0935 (ISSN-L)
- 1432-2048
- OpenAlex
- W2050609428
- Cited by
- 31
- References
- 32
- Citations by year
- 2025: 1 · 2024: 3 · 2023: 3 · 2022: 3 · 2020: 1 · 2019: 2 · 2018: 4 · 2017: 1 · 2016: 6 · 2015: 2 · 2014: 1 · 2013: 3 · 2012: 1
- FWCI
- 2.55
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Planta (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- Japan Society for the Promotion of Science (23570065, 23120523, 20227001)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Keywords
- F-actin, microtubules, Physcomitrella patens, bryophytes, Adiantum capillus-veneris, cytoskeletal inhibitors, cytochalasin B, oryzalin, chloroplast positioning
- MeSH terms
- Actins, Chloroplasts, Cloning, Molecular, Light, Actin Cytoskeleton, Gene Expression, Sequence Deletion, Sequence Analysis, DNA, Protein Structure, Tertiary, DNA, Complementary, Bryopsida, Ferns, Chloroplast Proteins
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