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A novel monoclonal antibody against human Argonaute proteins reveals unexpected characteristics of miRNAs in human blood cells
Abstract
Argonaute (Ago) proteins bind to microRNA (miRNAs) and short interfering RNAs (siRNAs) and form the core components of effector complexes that mediate miRNA and siRNA function. Currently, there is a paucity of reliable antibodies against mammalian Ago proteins, thus precluding studies of endogenous Ago proteins from tissues. Here we report the development of 2A8, a novel anti-Ago monoclonal antibody that recognizes human and mouse Ago proteins and efficiently immunoprecipitates miRNAs. We report the characterization of 2A8 and its use to clone miRNAs from human brain and from preparations of human polymorphonuclear leukocytes (neutrophils), which revealed a prevalent miRNA with unusual features.
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“The AGO antibody 2A8 that we used in this study was raised in mice immunized with recombinant human AGO2 protein, but the 2A8 antibody recognizes all 4 human AGO proteins (AGO1, AGO2, AGO3, and AGO4) [47].”
From The Mitochondria-Associated ER Membranes Are Novel Subcellular Locations Enriched for Inflammatory-Responsive MicroRNAs (2020) · cites this paper as [47]
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Chi, S. W., Hannon, G. J., & Darnell, R. B. (2012) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.2230 · Cited by 328
Moore, M. J., Scheel, T. K. H., Luna, J. M., Park, C. Y., Fak, J. J., Nishiuchi, E., Rice, C. M., & Darnell, R. B. (2015) · Nature Communications · doi:10.1038/ncomms9864 · Cited by 326
Kundu, P., Fabian, M. R., Sonenberg, N., Bhattacharyya, S. N., & Filipowicz, W. (2012) · Nucleic Acids Research · doi:10.1093/nar/gks148 · Cited by 180
Truesdell, S. S., Mortensen, R. D., Seo, M., Schroeder, J. C., Lee, J. H., LeTonqueze, O., & Vasudevan, S. (2012) · Scientific Reports · doi:10.1038/srep00842 · Cited by 173
Wang, W.-X., Prajapati, P., Nelson, P. T., & Springer, J. E. (2020) · Molecular Neurobiology · doi:10.1007/s12035-020-01937-y · Cited by 49
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Metadata
- Authors
- Nelson, P. T., De Planell-Saguer, M., Lamprinaki, S., Kiriakidou, M., Zhang, P., O'Doherty, U., & Mourelatos, Z.
- Venue
- RNA
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2007-08-24
- Volume
- 13
- Issue
- 10
- Pages
- 1787-1792
- ISSN
- 1355-8382
- 1469-9001
- Cited by
- 103
- References
- 1
- Language
- en
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