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Arabidopsis TFL2/LHP1 Specifically Associates with Genes Marked by Trimethylation of Histone H3 Lysine 27

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Turck, Franziska, Roudier, François, Farrona, Sara, Martin-Magniette, Marie-Laure, Guillaume, Elodie, Buisine, Nicolas, Gagnot, Séverine, Martienssen, Robert A, Coupland, George, Colot, Vincent·2007·PLoS Genetics
DOI 10.1371/journal.pgen.0030086
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Abstract

TERMINAL FLOWER 2/LIKE HETEROCHROMATIN PROTEIN 1 (TFL2/LHP1) is the only Arabidopsis protein with overall sequence similarity to the HETEROCHROMATIN PROTEIN 1 (HP1) family of metazoans and S. pombe. TFL2/LHP1 represses transcription of numerous genes, including the flowering-time genes FLOWERING LOCUS T (FT) and FLOWERING LOCUS C (FLC), as well as the floral organ identity genes AGAMOUS (AG) and APETALA 3 (AP3). These genes are also regulated by proteins of the Polycomb repressive complex 2 (PRC2), and it has been proposed that TFL2/LHP1 represents a potential stabilizing factor of PRC2 activity. Here we show by chromatin immunoprecipitation and hybridization to an Arabidopsis Chromosome 4 tiling array (ChIP-chip) that TFL2/LHP1 associates with hundreds of small domains, almost all of which correspond to genes located within euchromatin. We investigated the chromatin marks to which TFL2/LHP1 binds and show that, in vitro, TFL2/LHP1 binds to histone H3 di- or tri-methylated at lysine 9 (H3K9me2 or H3K9me3), the marks recognized by HP1, and to histone H3 trimethylated at lysine 27 (H3K27me3), the mark deposited by PRC2. However, in vivo TFL2/LHP1 association with chromatin occurs almost exclusively and co-extensively with domains marked by H3K27me3, but not H3K9me2 or -3. Moreover, the distribution of H3K27me3 is unaffected in lhp1 mutant plants, indicating that unlike PRC2 components, TFL2/LHP1 is not involved in the deposition of this mark. Rather, our data suggest that TFL2/LHP1 recognizes specifically H3K27me3 in vivo as part of a mechanism that represses the expression of many genes targeted by PRC2.

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Metadata

Authors
Turck, F., Roudier, F., Farrona, S., Martin-Magniette, M.-L., Guillaume, E., Buisine, N., Gagnot, S., Martienssen, R. A., Coupland, G., & Colot, V.
Venue
PLoS Genetics
Publisher
Public Library of Science (PLoS)
Published
2007-06-01
Volume
3
Issue
6
Pages
e86
ISSN
  • 1553-7390 (ISSN-L)
  • 1553-7404
DOI
10.1371/journal.pgen.0030086
OpenAlex
W2031012791
Cited by
638
References
2
FWCI
52.36
Type
journal article
Language
en
Open access
gold
Licence
creativecommons.org/licenses/by/4.0/ (unspecified)

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CoChat. (2026). Citation check: Arabidopsis TFL2/LHP1 Specifically Associates with Genes Marked by Trimethylation of Histone H3 Lysine 27. https://app.cochat.ai/papers/10.1371/journal.pgen.0030086
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Turck, F., Roudier, F., Farrona, S., Martin-Magniette, M.-L., Guillaume, E., Buisine, N., Gagnot, S., Martienssen, R. A., Coupland, G., & Colot, V. (2007). Arabidopsis TFL2/LHP1 Specifically Associates with Genes Marked by Trimethylation of Histone H3 Lysine 27. PLoS Genetics. https://doi.org/10.1371/journal.pgen.0030086

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