Overcoming Intrinsic Restriction Enzyme Barriers Enhances Transformation Efficiency in Arthrospira platensis C1
Open accessAbstract
The development of a reliable genetic transformation system for Arthrospira platensis has been a long-term goal, mainly for those trying either to improve its performance in large-scale cultivation systems or to enhance its value as food and feed additives. However, so far, most of the attempts to develop such a transformation system have had limited success. In this study, an efficient and stable transformation system for A. platensis C1 was successfully developed. Based on electroporation and transposon techniques, exogenous DNA could be transferred to and stably maintained in the A. platensis C1 genome. Most strains of Arthrospira possess strong restriction barriers, hampering the development of a gene transfer system for this group of cyanobacteria. By using a type I restriction inhibitor and liposomes to protect the DNA from nuclease digestion, the transformation efficiency was significantly improved. The transformants were able to grow on a selective medium for more than eight passages, and the transformed DNA could be detected from the stable transformants. We propose that the intrinsic endonuclease enzymes, particularly the type I restriction enzyme, in A. platensis C1 play an important role in the transformation efficiency of this industrial important cyanobacterium.
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Only after a decade was an efficient and stable transformation method of spirulina reported.
“Only after a decade was an efficient and stable transformation method of spirulina reported [109].”
From Spirulina/Arthrospira/Limnospira—Three Names of the Single Organism (2024) · cites this paper as [109]
An effective transformation system for A. platensis has been developed using a type 1 restriction inhibitor and liposomes to protect exogenous DNA.
“Recently, an effective transformation system for A. platensis has been developed using a type 1 restriction inhibitor and liposomes to protect the exogenous DNA 28”
From The Draft Genome of a Hydrogen-producing Cyanobacterium, <i>Arthrospira platensis</i> NIES-46 (2019) · cites this paper as [28]
Genetic engineering can make microalgae more resistant against contamination.
“Another approach to avoid contamination is genetic engineering by making the microalgae more resistant against contamination [192, 193].”
From Lab-scale photobioreactor systems: principles, applications, and scalability (2022) · cites this paper as [193]
The Introduction describes genetic modification of Arthrospira platensis, and the section on integrated genes reports spectinomycin resistance in transformants. Neither passage addresses resistance to contamination or genetic engineering as a way to avoid contamination (searched: contamination, contaminant resistance, outdoor cultivation). (LLM, conf=0.73)
Experimental — accuracy not yet measuredThe scarce availability of genetic tools for L. platensis is a drawback.
“The scarce availability of genetic tools for this species is still a drawback.”
From Characterization of Limnospira platensis PCC 9108 R-M and CRISPR-Cas systems (2024) · cites this paper as Jeamton et al., 2017
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- Authors
- Jeamton, W., Dulsawat, S., Tanticharoen, M., Vonshak, A., & Cheevadhanarak, S.
- Affiliations
- King Mongkut's University of Technology Thonburi (TH)
- Ben-Gurion University of the Negev (IL)
- Corresponding author
- Cheevadhanarak, Supapon
- ORCID
- Vonshak, Avigad
- Venue
- Plant and Cell Physiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2017-02-01
- Volume
- 58
- Issue
- 4
- Pages
- 822-830
- ISSN
- 0032-0781 (ISSN-L)
- 1471-9053
- OpenAlex
- W2585355610
- Cited by
- 31
- References
- 58
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 4 · 2023: 4 · 2022: 3 · 2021: 6 · 2020: 3 · 2019: 2 · 2018: 2 · 2017: 2
- FWCI
- 1.63
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Plant and Cell Physiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Advanced Industrial Science and Technology
- Magyar Tudományos Akadémia
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Keywords
- Spirulina platensis, genetic transformation, type I restriction enzymes, electroporation, transposable elements, liposomes, cyanobacteria, transformation efficiency, stable transformants
- MeSH terms
- Spectinomycin, Bacterial Proteins, Culture Media, DNA Transposable Elements, Enzymes, Plasmids, Promoter Regions, Genetic, Transformation, Genetic, Reproducibility of Results, Polymerase Chain Reaction, Genome, Bacterial, Transposases, Drug Resistance, Bacterial, Spirulina
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