Cited by 106Cardiovascular and exercise physiologyAdipose Tissue and MetabolismDiabetes, Cardiovascular Risks, and Lipoproteins

Fat Oxidation, Fitness and Skeletal Muscle Expression of Oxidative/Lipid Metabolism Genes in South Asians: Implications for Insulin Resistance?

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Hall, Lesley M. L., Moran, Colin N., Milne, Gillian R., Wilson, John, MacFarlane, Niall G., Forouhi, Nita G., Hariharan, Narayanan, Salt, Ian P. +2 more·2010·PLoS ONE

Abstract

BACKGROUND: South Asians are more insulin resistant than Europeans, which cannot be fully explained by differences in adiposity. We investigated whether differences in oxidative capacity and capacity for fatty acid utilisation in South Asians might contribute, using a range of whole-body and skeletal muscle measures. METHODOLOGY/PRINCIPAL FINDINGS: Twenty men of South Asian ethnic origin and 20 age and BMI-matched men of white European descent underwent exercise and metabolic testing and provided a muscle biopsy to determine expression of oxidative and lipid metabolism genes and of insulin signalling proteins. In analyses adjusted for age, BMI, fat mass and physical activity, South Asians, compared to Europeans, exhibited; reduced insulin sensitivity by 26% (p = 0.010); lower VO2max (40.6±6.6 vs 52.4±5.7 ml x kg(-1) x min(-1), p = 0.001); and reduced fat oxidation during submaximal exercise at the same relative (3.77±2.02 vs 6.55±2.60 mg x kg(-1) x min(-1) at 55% VO2max, p = 0.013), and absolute (3.46±2.20 vs 6.00±1.93 mg x kg(-1) x min(-1) at 25 ml O(2) x kg(-1) x min(-1), p = 0.021), exercise intensities. South Asians exhibited significantly higher skeletal muscle gene expression of CPT1A and FASN and significantly lower skeletal muscle protein expression of PI3K and PKB Ser473 phosphorylation. Fat oxidation during submaximal exercise and VO2max both correlated significantly with insulin sensitivity index and PKB Ser473 phosphorylation, with VO2max or fat oxidation during exercise explaining 10-13% of the variance in insulin sensitivity index, independent of age, body composition and physical activity. CONCLUSIONS/SIGNIFICANCE: These data indicate that reduced oxidative capacity and capacity for fatty acid utilisation at the whole body level are key features of the insulin resistant phenotype observed in South Asians, but that this is not the consequence of reduced skeletal muscle expression of oxidative and lipid metabolism genes.

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  1. [28]Frayn KN Macdonald IA 1997 Assessment of substrate and energy metabolism in vivo. Draznin B Rizza R Clinical Research in Diabetes and Obesity Part I: Methods, Assessment, and Metabolic Regulation Totowa NJ Humana Press 101 124

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Authors
Hall, L. M. L., Moran, C. N., Milne, G. R., Wilson, J., MacFarlane, N. G., Forouhi, N. G., Hariharan, N., Salt, I. P., Sattar, N., & Gill, J. M. R.
Affiliations
  • University of Glasgow (GB)
  • Addenbrooke's Hospital (GB)
  • Pfizer (United States) (US)
Corresponding author
Gill, Jason M. R.
Venue
PLoS ONE
Published
2010-12-01
Volume
5
Issue
12
Pages
e14197
ISSN
1932-6203 (ISSN-L)
OpenAlex
W2071313377
Cited by
106
References
76
Citations by year
2026: 4 · 2025: 3 · 2024: 2 · 2023: 4 · 2022: 7 · 2021: 9 · 2020: 8 · 2019: 9 · 2018: 8 · 2017: 9 · 2016: 9 · 2015: 12 · 2014: 3 · 2013: 10 · 2012: 8
FWCI
16.27
Type
journal article
Language
en
Open access
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • ENLIGHTEN (Jurnal Bimbingan dan Konseling Islam) (acceptedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Stirling Online Research Repository (University of Stirling) (submittedVersion, cc-by)
Funders
  • Pfizer
  • Diabetes UK (06/0003224)
Topics
  • Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes, Cardiovascular Risks, and Lipoproteins
Keywords
South Asians, insulin resistance, fat oxidation, skeletal muscle gene expression, lipid metabolism genes, VO2max, exercise capacity, CPT1A, fatty acid synthase
MeSH terms
Adipose Tissue, Adult, Anthropometry, Asia, Biopsy, Body Composition, Fatty Acids, Female, Humans, Insulin, Insulin Resistance, Male, Oxygen, Signal Transduction, Body Mass Index, Muscle, Skeletal, Lipid Metabolism

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