Panton-Valentine leukocidin does play a role in the early stage of Staphylococcus aureus skin infections: a rabbit model
Open accessAbstract
Despite epidemiological data linking necrotizing skin infections with the production of Panton-Valentine leukocidin (PVL), the contribution of this toxin to the virulence of S. aureus has been highly discussed as a result of inconclusive results of in vivo studies. However, the majority of these results originate from experiments using mice, an animal species which neutrophils--the major target cells for PVL--are highly insensitive to the action of this leukocidin. In contrast, the rabbit neutrophils have been shown to be as sensitive to PVL action as human cells, making the rabbit a better experimental animal to explore the PVL role. In this study we examined whether PVL contributes to S. aureus pathogenicity by means of a rabbit skin infection model. The rabbits were injected intradermally with 10(8) cfu of either a PVL positive community-associated methicillin-resistant S. aureus isolate, its isogenic PVL knockout or a PVL complemented knockout strain, and the development of skin lesions was observed. While all strains induced skin infection, the wild type strain produced larger lesions and a higher degree of skin necrosis compared to the PVL knockout strain in the first week after the infection. The PVL expression in the rabbits was indirectly confirmed by a raise in the serum titer of anti-LukS-PV antibodies observed only in the rabbits infected with PVL positive strains. These results indicate that the rabbit model is more suitable for studying the role of PVL in staphylococcal diseases than other animal models. Further, they support the epidemiological link between PVL producing S. aureus strains and necrotizing skin infections.
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pvl is important in skin necrosis.
“Recent studies showing the importance of pvl in skin necrosis [55] confirmed that alternative animal models are more appropriate for studying S. aureus skin infection and therefore the porcine wound model due to its many similarities to wound healing in humans offers multiple advantages.”
From Interactions of Methicillin Resistant Staphylococcus aureus USA300 and Pseudomonas aeruginosa in Polymicrobial Wound Infection (2013) · cites this paper as [55]
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Cites
Staphylococcus aureus Infections
Lowy, F. D. (1998) · New England Journal of Medicine · doi:10.1056/nejm199808203390806 · Cited by 6,018 · see citing works
Lina, G., Piemont, Y., Godail-Gamot, F., Bes, M., Peter, M.-O., Gauduchon, V., Vandenesch, F., & Etienne, J. (1999) · Clinical Infectious Diseases · doi:10.1086/313461 · Cited by 2,489 · see citing works
Self-citationGillet, Y., Issartel, B., Vanhems, P., Fournet, J.-C., Lina, G., Bes, M., Vandenesch, F., Piémont, Y., Brousse, N., Floret, D., et al. (2002) · The Lancet · doi:10.1016/s0140-6736(02)07877-7 · Cited by 1,514 · see citing works
Self-citationMiller, L. G., Perdreau-Remington, F., Rieg, G., Mehdi, S., Perlroth, J., Bayer, A. S., Tang, A. W., Phung, T. O., & Spellberg, B. (2005) · New England Journal of Medicine · doi:10.1056/nejmoa042683 · Cited by 1,053 · see citing works
Wang, R., Braughton, K. R., Kretschmer, D., Bach, T.-H. L., Queck, S. Y., Li, M., Kennedy, A. D., Dorward, D. W., Klebanoff, S. J., Peschel, A., et al. (2007) · Nature Medicine · doi:10.1038/nm1656 · Cited by 1,046 · see citing works
Voyich, J. M., Otto, M. J., Mathema, B., Braughton, K. R., Whitney, A. R., Welty, D. M., Long, R. D., Dorward, D. W., Gardner, D. J., Lina, G., et al. (2006) · The Journal of Infectious Diseases · doi:10.1086/509506 · Cited by 562 · see citing works
Self-citationMethicillin-resistant Staphylococcus aureus strain USA300: origin and epidemiology
Tenover, F. C., & Goering, R. V. (2009) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dkp241 · Cited by 443 · see citing works
Löffler, B., Hussain, M. S., Grundmeier, M., Brück, M., Holzinger, D., Varga, G., Roth, J., Kahl, B. C., Proctor, R. A., & Peters, G. (2010) · PLoS Pathogens · doi:10.1371/journal.ppat.1000715 · Cited by 440 · see citing works
Diep, B. A., Chan, L., Tattevin, P., Kajikawa, O., Martin, T. R., Basuino, L., Mai, T. T., Marbach, H., Braughton, K. R., Whitney, A. R., et al. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0912403107 · Cited by 347 · see citing works
Self-citation(2014) · Elsevier eBooks · doi:10.1016/c2011-0-05821-0 · Cited by 335 · see citing works
Epidemiological data on Staphylococcus aureus strains producing synergohymenotropic toxins
Prevost, G., Couppie, P., Prevost, P., Gayet, S., Petiau, P., Cribier, B., Monteil, H., & Piemont, Y. (1995) · Journal of Medical Microbiology · doi:10.1099/00222615-42-4-237 · Cited by 269 · see citing works
Diep, B. A., Sensabaugh, G. F., Somboona, N. S., Carleton, H. A., & Perdreau‐Remington, F. (2004) · Journal of Clinical Microbiology · doi:10.1128/jcm.42.5.2080-2084.2004 · Cited by 254 · see citing works
Wardenburg, J. B., Palazzolo‐Ballance, A. M., Otto, M., Schneewind, O., & DeLeo, F. R. (2008) · The Journal of Infectious Diseases · doi:10.1086/592053 · Cited by 236 · see citing works
Diep, B. A., Palazzolo‐Ballance, A. M., Tattevin, P., Basuino, L., Braughton, K. R., Whitney, A. R., Chen, L., Kreiswirth, B. N., Otto, M. J., DeLeo, F. R., et al. (2008) · PLoS ONE · doi:10.1371/journal.pone.0003198 · Cited by 188 · see citing works
Li, M., Cheung, G. Y. C., Hu, J., Wang, D., Joo, H., DeLeo, F. R., & Otto, M. (2010) · The Journal of Infectious Diseases · doi:10.1086/657419 · Cited by 175 · see citing works
Brown, E. L., Dumitrescu, O., Thomas, D. Y., Badiou, C., Koers, E. M., Choudhury, P., Vazquez, V., Étienne, J., Lina, G., Vandenesch, F., et al. (2008) · Clinical Microbiology and Infection · doi:10.1111/j.1469-0691.2008.02648.x · Cited by 162 · see citing works
Self-citationStaphylococcus aureus Leukocidin: A New Virulence Factor in Cutaneous Infections?
Cribier, B. J., Prévost, G., Couppié, P., Finck‐Barbançon, V., Grosshans, E., & Piémont, Y. (2009) · Dermatology · doi:10.1159/000247443 · Cited by 159 · see citing works
Crémieux, A., Dumitrescu, O., Lina, G., Vallée, C. A., Côté, J.-F., Muffat‐Joly, M., Lilin, T., Étienne, J., Vandenesch, F., & Saleh‐Mghir, A. (2009) · PLoS ONE · doi:10.1371/journal.pone.0007204 · Cited by 127 · see citing works
Self-citationGladstone, G. P., & HEYNINGEN, W. E. V. (1957) · PubMed · Cited by 125 · see citing works
Leukocidin from Staphylococcus aureus and cutaneous infections: an epidemiologic study
Couppié, P. (1994) · Archives of Dermatology · doi:10.1001/archderm.130.9.1208 · Cited by 102 · see citing works
Tseng, C. W., Kyme, P. A., Low, J., Rocha, M. A., Alsabeh, R., Miller, L. G., Otto, M. J., Arditi, M., Diep, B. A., Nizet, V., et al. (2009) · PLoS ONE · doi:10.1371/journal.pone.0006387 · Cited by 102 · see citing works
Fractionation of a leucocidin from Staphylococcus aureus
Woodin, A. M. (1959) · Biochemical Journal · doi:10.1042/bj0730225 · Cited by 93 · see citing works
Hongo, I., Baba, T., Oishi, K., Morimoto, Y., Ito, T., & Hiramatsu, K. (2009) · The Journal of Infectious Diseases · doi:10.1086/605332 · Cited by 80 · see citing works
Rabbit staphylococcosis: difficult solutions for serious problems
Hermans, K., Devriese, L. A., & Haesebrouck, F. (2002) · Veterinary Microbiology · doi:10.1016/s0378-1135(02)00260-2 · Cited by 77 · see citing works
Self-citationThe neurotoxin of Shigella shigae. I. Production, purification and properties of the toxin.
HEYNINGEN, W. E. V., & Gp, G. (1953) · PubMed · Cited by 69 · see citing works
Hermans, K., Herdt, P. D., Devriese, L. A., et al. (1999) · Veterinary Microbiology · doi:10.1016/s0378-1135(99)00028-0 · Cited by 51 · see citing works
Self-citationCroze, M., Dauwalder, O., Dumitrescu, O., Badiou, C., Gillet, Y., Genestier, A., Vandenesch, F., Étienne, J., & Lina, G. (2009) · Clinical Microbiology and Infection · doi:10.1111/j.1469-0691.2008.02650.x · Cited by 39 · see citing works
Self-citationSzmigielski, S., Sobiczewska, E., Prévost, G., Monteil, H., Colin, D. A., & Jeljaszewicz, J. (1998) · Zentralblatt für Bakteriologie · doi:10.1016/s0934-8840(98)80012-1 · Cited by 30 · see citing works
Multiplex PCR assay for the detection of high virulence rabbit Staphylococcus aureus strains
Vancraeynest, D., Haesebrouck, F., & Hermans, K. (2006) · Veterinary Microbiology · doi:10.1016/j.vetmic.2006.12.011 · Cited by 24 · see citing works
Self-citationThe rabbit consultation and clinical techniques
Harcourt-Brown, F. M. (2002) · Elsevier eBooks · doi:10.1016/b978-075064002-2.50006-0 · Cited by 24 · see citing works
de Horts, T. B., Dumitrescu, O., Badiou, C., Thomas, D. Y., Benito, Y., Étienne, J., Vandenesch, F., & Lina, G. (2009) · Infection and Immunity · doi:10.1128/iai.00843-09 · Cited by 16 · see citing works
Self-citation
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Research works printed but not linked: 1
- [8]Prévost G Supersac G Colin DA Couppie P Sire S 1994 The new family of leucotoxins from Staphylococcus aureus - structural and biological properties. Freer J Aitken R Alouf JE Boulnois G Falmagne P Bacterial Protein Toxins - Sixth European Workshop Stuttgart 70 Gustav Fischer Verlag 284 293
Cited by
Sorted by citations
Vandenesch, F., Lina, G., & Henry, T. (2012) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2012.00012 · Cited by 405 · see citing works
Self-citationPastar, I., Nusbaum, A. G., Gil, J., Patel, S. B., Chen, J., Valdes, J., Stojadinovic, O., Plano, L. R., Tomic-Canic, M., & Davis, S. C. (2013) · PLoS ONE · doi:10.1371/journal.pone.0056846 · Cited by 391 · see citing works
Neutrophils in innate host defense against Staphylococcus aureus infections
Rigby, K. M., & DeLeo, F. R. (2012) · Seminars in Immunopathology · doi:10.1007/s00281-011-0295-3 · Cited by 400 · see citing works
Methicillin-Resistant Staphylococcus aureus: An Evolving Pathogen
Stryjewski, M. E., & Corey, G. R. (2014) · Clinical Infectious Diseases · doi:10.1093/cid/cit613 · Cited by 334 · see citing works
Spaan, A. N., Vrieling, M., Wallet, P., Badiou, C., Reyes-Robles, T., Ohneck, E. A., Benito, Y., de Haas, C. J. C., Day, C. J., Jennings, M. P., et al. (2014) · Nature Communications · doi:10.1038/ncomms6438 · Cited by 166 · see citing works
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- Authors
- Lipinska, U., Hermans, K., Meulemans, L., Dumitrescu, O., Badiou, C., Duchateau, L., Haesebrouck, F., Etienne, J., & Lina, G.
- Affiliations
- Ghent University (BE)
- Lyon 1 Université (FR)
- Corresponding author
- Lipinska, Urszula
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2011-08-05
- Volume
- 6
- Issue
- 8
- Pages
- e22864
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC3151264
- OpenAlex
- W1988430837
- Cited by
- 100
- References
- 31
- Citations by year
- 2025: 5 · 2024: 1 · 2023: 4 · 2022: 3 · 2021: 5 · 2020: 3 · 2019: 1 · 2018: 7 · 2017: 4 · 2016: 5 · 2015: 9 · 2014: 17 · 2013: 21 · 2012: 10
- FWCI
- 11.45
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Ghent University Academic Bibliography (Ghent University) (submittedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- Pfizer
- Universiteit Gent
- Bijzonder Onderzoeksfonds UGent
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Streptococcal Infections and Treatments
- Health Sciences › Medicine › Infectious Diseases › Clostridium difficile and Clostridium perfringens research
- Keywords
- Panton-Valentine leukocidin, Staphylococcus aureus, necrotizing skin infection, virulence factors, neutrophil lysis, community-associated methicillin-resistant Staphylococcus aureus, skin necrosis
- MeSH terms
- Animals, Bacterial Toxins, Disease Models, Animal, Exotoxins, Female, Leukocidins, Male, Rabbits, Staphylococcal Infections, Staphylococcal Skin Infections, Staphylococcus aureus, Methicillin-Resistant Staphylococcus aureus
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