Cited by 47Circadian rhythm and melatoninReproductive biology and impacts on aquatic speciesPhysiological and biochemical adaptations

Lunar phase-dependent expression of cryptochrome and a photoperiodic mechanism for lunar phase-recognition in a reef fish, goldlined spinefoot

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Fukushiro, Masato, Takeuchi, Takahiro, Takeuchi, Yuki, Hur, Sung-Pyo, Sugama, Nozomi, Takemura, Akihiro, Kubo, Yoko, Okano, Keiko +1 more·2011·PLoS ONE

Abstract

Lunar cycle-associated physiology has been found in a wide variety of organisms. Recent study has revealed that mRNA levels of Cryptochrome (Cry), one of the circadian clock genes, were significantly higher on a full moon night than on a new moon night in coral, implying the involvement of a photoreception system in the lunar-synchronized spawning. To better establish the generalities surrounding such a mechanism and explore the underlying molecular mechanism, we focused on the relationship between lunar phase, Cry gene expression, and the spawning behavior in a lunar-synchronized spawner, the goldlined spinefoot (Siganus guttatus), and we identified two kinds of Cry genes in this animal. Their mRNA levels showed lunar cycle-dependent expression in the medial part of the brain (mesencephalon and diencephalon) peaking at the first quarter moon. Since this lunar phase coincided with the reproductive phase of the goldlined spinefoot, Cry gene expression was considered a state variable in the lunar phase recognition system. Based on the expression profiles of SgCrys together with the moonlight's pattern of timing and duration during its nightly lunar cycle, we have further speculated on a model of lunar phase recognition for reproductive control in the goldlined spinefoot, which integrates both moonlight and circadian signals in a manner similar to photoperiodic response.

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  • In Siganus guttatus, cry1 and cry3 transcripts in both brain and ovaries exhibited an overall increase in the brain during NM and especially FQM, and lower levels at LQM.

    “In the golden rabbitfish, Siganus guttatus, which spawns around FQM [47,48], cry1 and cry3 transcripts in both brain and ovaries were analyzed, exhibiting an overall increase in the brain (mesencephalon and diencephalon) during NM and especially FQM, and lower levels at LQM.”
  • In S. guttatus, mRNA levels of SgCry1 and SgCry3 fluctuate with the lunar cycle in the brain, but not the ovary.

    “In the golden rabbit fish, S. guttatus, mRNA levels of two tr-cry homologs—SgCry1 and SgCry3—fluctuate with the lunar cycle in the brain, but not the ovary (30).”

    From An Overview of Monthly Rhythms and Clocks (2017) · cites this paper as [30]

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Research works printed but not linked: 2

  1. [1]Domeier ML Colin PL 1997 Tropical reef fish spawning aggregations: Defined and reviewed. Bulletin of Marine Science 60 698 726
  2. [6]Colin PL Shapiro DY Weiler D 1987 Aspects of the reproduction of two groupers, Epinephelus guttatus and E. striatus in the West Indies. Bulletin of Marine Science 40 220 230

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Authors
Fukushiro, M., Takeuchi, T., Takeuchi, Y., Hur, S.-P., Sugama, N., Takemura, A., Kubo, Y., Okano, K., & Okano, T.
Affiliations
  • Waseda University (JP)
  • University of the Ryukyus (JP)
Corresponding author
Okano, Toshiyuki
Venue
PLoS ONE
Published
2011-12-07
Volume
6
Issue
12
Pages
e28643
ISSN
1932-6203 (ISSN-L)
OpenAlex
W2051540120
Cited by
47
References
45
Citations by year
2026: 2 · 2025: 2 · 2023: 5 · 2022: 3 · 2021: 2 · 2020: 7 · 2019: 5 · 2018: 3 · 2017: 5 · 2016: 1 · 2015: 2 · 2014: 7 · 2013: 2 · 2012: 1
FWCI
1.48
Type
journal article
Language
en
Open access
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
  • Figshare (submittedVersion, cc-by)
Funders
  • Ministry of Education, Culture, Sports, Science and Technology
  • Japan Society for the Promotion of Science (22657042, 22405029)
Topics
  • Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Reproductive biology and impacts on aquatic species
  • Physical Sciences › Environmental Science › Ecology › Physiological and biochemical adaptations
Keywords
cryptochrome, lunar phases, photoperiodism, Siganus guttatus, clock genes, gene expression, moonlight, reproductive control, coral spawning, photoreception
MeSH terms
Animals, Brain, Circadian Rhythm, Cloning, Molecular, Female, Fishes, Light, Ovary, Phylogeny, RNA, Messenger, Moon, Photoperiod, DNA Primers, Cryptochromes

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