The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.
Extracellular Vesicles from Parasitic Helminths Contain Specific Excretory/Secretory Proteins and Are Internalized in Intestinal Host Cells
Open accessAbstract
The study of host-parasite interactions has increased considerably in the last decades, with many studies focusing on the identification of parasite molecules (i.e. surface or excretory/secretory proteins (ESP)) as potential targets for new specific treatments and/or diagnostic tools. In parallel, in the last few years there have been significant advances in the field of extracellular vesicles research. Among these vesicles, exosomes of endocytic origin, with a characteristic size ranging from 30-100 nm, carry several atypical secreted proteins in different organisms, including parasitic protozoa. Here, we present experimental evidence for the existence of exosome-like vesicles in parasitic helminths, specifically the trematodes Echinostoma caproni and Fasciola hepatica. These microvesicles are actively released by the parasites and are taken up by host cells. Trematode extracellular vesicles contain most of the proteins previously identified as components of ESP, as confirmed by proteomic, immunogold labeling and electron microscopy studies. In addition to parasitic proteins, we also identify host proteins in these structures. The existence of extracellular vesicles explains the secretion of atypical proteins in trematodes, and the demonstration of their uptake by host cells suggests an important role for these structures in host-parasite communication, as described for other infectious agents.
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Cites
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Cited by
Biogenesis, Secretion, and Intercellular Interactions of Exosomes and Other Extracellular Vesicles
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Metadata
- Authors
- Marcilla, A., Trelis, M., Cortés, A., Sotillo, J., Cantalapiedra, F., Minguez, M. T., Valero, M. L., Sánchez del Pino, M. M., Muñoz-Antoli, C., Toledo, R., & Bernal, D.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2012-09-24
- Volume
- 7
- Issue
- 9
- Pages
- e45974
- ISSN
- 1932-6203 (ISSN-L)
- OpenAlex
- W2038944440
- Cited by
- 349
- References
- 43
- FWCI
- 6.27
- Type
- journal article
- Language
- en
- Open access
- gold
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- The paper
- Marcilla, A., Trelis, M., Cortés, A., Sotillo, J., Cantalapiedra, F., Minguez, M. T., Valero, M. L., Sánchez del Pino, M. M., Muñoz-Antoli, C., Toledo, R., & Bernal, D. (2012). Extracellular Vesicles from Parasitic Helminths Contain Specific Excretory/Secretory Proteins and Are Internalized in Intestinal Host Cells. PLoS ONE. https://doi.org/10.1371/journal.pone.0045974
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
