Differential Effects of Antibiotic Therapy on the Structure and Function of Human Gut Microbiota
Open accessAbstract
The human intestinal microbiota performs many essential functions for the host. Antimicrobial agents, such as antibiotics (AB), are also known to disturb microbial community equilibrium, thereby having an impact on human physiology. While an increasing number of studies investigate the effects of AB usage on changes in human gut microbiota biodiversity, its functional effects are still poorly understood. We performed a follow-up study to explore the effect of ABs with different modes of action on human gut microbiota composition and function. Four individuals were treated with different antibiotics and samples were taken before, during and after the AB course for all of them. Changes in the total and in the active (growing) microbiota as well as the functional changes were addressed by 16S rRNA gene and metagenomic 454-based pyrosequencing approaches. We have found that the class of antibiotic, particularly its antimicrobial effect and mode of action, played an important role in modulating the gut microbiota composition and function. Furthermore, analysis of the resistome suggested that oscillatory dynamics are not only due to antibiotic-target resistance, but also to fluctuations in the surviving bacterial community. Our results indicated that the effect of AB on the human gut microbiota relates to the interaction of several factors, principally the properties of the antimicrobial agent, and the structure, functions and resistance genes of the microbial community.
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Medication, especially chronic medication, can exert a strong impact on intestinal microbiota.
“Medication, especially chronic medication, can exert a strong impact on intestinal microbiota [19] .”
From Intestinal microbiota in health and disease: Role of bifidobacteria in gut homeostasis (2014) · cites this paper as [19]
Antibiotic treatment can kill members of the gut microbiota and reduce competition for nutrients, making these nutrients available to support the growth of pathogens.
“However, antibiotic treatment can kill members of the gut microbiota (‘gut commensals’) and reduce competition for nutrients, making these nutrients available to support the growth of pathogens.”
From Role of the gut microbiota in nutrient competition and protection against intestinal pathogen colonization (2023) · cites this paper as [29]
Antibiotic usage is widely documented to cause a substantial shift in the composition and functionality of the gut microbiota.
“Antibiotic usage is widely documented to cause a substantial shift in the composition and functionality of the gut microbiota [29].”
From Role of the gut microbiota in nutrient competition and protection against intestinal pathogen colonization (2023) · cites this paper as [29]
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- Authors
- Pérez-Cobas, A. E., Artacho, A., Knecht, H., Ferrús, M. L., Friedrichs, A., Ott, S. J., Moya, A., Latorre, A., & Gosalbes, M. J.
- Affiliations
- Universitat de València (ES)
- Christian-Albrechts-Universität zu Kiel (DE)
- Corresponding author
- Latorre, Amparo
- Gosalbes, María José
- ORCID
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2013-11-25
- Volume
- 8
- Issue
- 11
- Pages
- e80201
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC3839934
- OpenAlex
- W2011482814
- Cited by
- 212
- References
- 56
- Citations by year
- 2026: 3 · 2025: 13 · 2024: 11 · 2023: 17 · 2022: 15 · 2021: 20 · 2020: 31 · 2019: 23 · 2018: 15 · 2017: 11 · 2016: 19 · 2015: 19 · 2014: 15
- FWCI
- 7.92
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Figshare (submittedVersion, cc-by)
- Repository of Digital Objects for Teaching Research and Culture (University of Valencia) (submittedVersion, cc-by-nc)
- Funders
- Ministerio de Economía y Competitividad (SAF2012, SAF 2012-31187)
- Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
- Keywords
- antibiotic therapy, gut microbiota, antibiotic mechanism of action, antibiotic classes, microbial community structure, microbial community function, resistome, antibiotic resistance genes, human microbiome
- MeSH terms
- Anti-Bacterial Agents, Follow-Up Studies, Humans, RNA, Ribosomal, 16S, Drug Resistance, Bacterial, Gastrointestinal Tract, Biodiversity, Metagenome, Microbiota
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