CoChat
Sign Up
Cited by 114melanin and skin pigmentationDermatologic Treatments and ResearchMelanoma and MAPK Pathways

The DOI matches its Crossref record; 64 of the 64 references it cites were found in a registry or an index.

Key Regulatory Role of Dermal Fibroblasts in Pigmentation as Demonstrated Using a Reconstructed Skin Model: Impact of Photo-Aging

Open access
Duval, Christine, Cohen, Catherine, Chagnoleau, Corinne, Flouret, Virginie, Bourreau, Emilie, Bernerd, Françoise·2014·PLoS ONE
DOI 10.1371/journal.pone.0114182PMC PMC4260844
Open this paper in CoChat — free accountOpen PDF

Abstract

To study cutaneous pigmentation in a physiological context, we have previously developed a functional pigmented reconstructed skin model composed of a melanocyte-containing epidermis grown on a dermal equivalent comprising living fibroblasts. The present studies, using the same model, aimed to demonstrate that dermal fibroblasts influence skin pigmentation up to the macroscopic level. The proof of principle was performed with pigmented skins differing only in the fibroblast component. First, the in vitro system was reconstructed with or without fibroblasts in order to test the global influence of the presence of this cell type. We then assessed the impact of the origin of the fibroblast strain on the degree of pigmentation using fetal versus adult fibroblasts. In both experiments, impressive variation in skin pigmentation at the macroscopic level was observed and confirmed by quantitative parameters related to skin color, melanin content and melanocyte numbers. These data confirmed the responsiveness of the model and demonstrated that dermal fibroblasts do indeed impact the degree of skin pigmentation. We then hypothesized that a physiological state associated with pigmentary alterations such as photo-aging could be linked to dermal fibroblasts modifications that accumulate over time. Pigmentation of skin reconstructed using young unexposed fibroblasts (n = 3) was compared to that of tissues containing natural photo-aged fibroblasts (n = 3) which express a senescent phenotype. A stimulation of pigmentation in the presence of the natural photo-aged fibroblasts was revealed by a significant increase in the skin color (decrease in Luminance) and an increase in both epidermal melanin content and melanogenic gene expression, thus confirming our hypothesis. Altogether, these data demonstrate that the level of pigmentation of the skin model is influenced by dermal fibroblasts and that natural photo-aged fibroblasts can contribute to the hyperpigmentation that is associated with photo-aging.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1371/journal.pone.0114182

Cites

64 of 64 references verified0 citation errors · 0 not checkable

Statistics

Figures

Figure 1 Microscopic examination and measurement of pigmentation in the in vitro pigmented skin model reconstructed in the presence or absence of fibroblasts in the dermal compartment. A) Microscopic examination of HES staining of cross-sections showed a correct epidermal architecture and differentiation process leading to the development of a stratum granulosum and stratum corneum in both 3D systems. The CD13 staining (green) of cross-sections confirmed the presence or absence of fibroblasts (from adult 21 yr-old donor) in the dermal equivalent. Macroscopic pictures of PRS illustrate the resulting drastic difference in pigmentation between the two conditions which was confirmed by B) Luminance measurement (mean of 3 experiments). Fontana-Masson staining for melanin granules detection (black points), and C) graph of melanin quantification (mean of 3 experiments) performed by image analysis of Fontana-Masson (FM) staining on cross-sections. To compare the two conditions, results on melanin content were normalized to the control condition (with fibroblasts). TRP-1 staining (red) of cross-sections shows melanocytes positioned at the basal layer of the epidermis in both 3D systems. TRP-1 staining (green) of PRS epidermal sheets and D) corresponding graph of melanocyte quantification (mean of 3 experiments) performed by image analysis. The values represent means ± standard deviation (SD). Statistical significance was evaluated by the Student's t-test; *** p-value <0.001. Magnification: A HES, FM = x400, CD13, TRP1/section = x200, TRP1/sheet = x50.
Figure 1 Microscopic examination and measurement of pigmentation in the in vitro pigmented skin model reconstructed in the presence or absence of fibroblasts in the dermal compartment. A) Microscopic examination of HES staining of cross-sections showed a correct epidermal architecture and differentiation process leading to the development of a stratum granulosum and stratum corneum in both 3D systems. The CD13 staining (green) of cross-sections confirmed the presence or absence of fibroblasts (from adult 21 yr-old donor) in the dermal equivalent. Macroscopic pictures of PRS illustrate the resulting drastic difference in pigmentation between the two conditions which was confirmed by B) Luminance measurement (mean of 3 experiments). Fontana-Masson staining for melanin granules detection (black points), and C) graph of melanin quantification (mean of 3 experiments) performed by image analysis of Fontana-Masson (FM) staining on cross-sections. To compare the two conditions, results on melanin content were normalized to the control condition (with fibroblasts). TRP-1 staining (red) of cross-sections shows melanocytes positioned at the basal layer of the epidermis in both 3D systems. TRP-1 staining (green) of PRS epidermal sheets and D) corresponding graph of melanocyte quantification (mean of 3 experiments) performed by image analysis. The values represent means ± standard deviation (SD). Statistical significance was evaluated by the Student's t-test; *** p-value <0.001. Magnification: A HES, FM = x400, CD13, TRP1/section = x200, TRP1/sheet = x50.CC BY · PubMed Central
Figure 2 Comparison of the effect of fibroblasts from fetal versus adult origin on reconstructed skin pigmentation. PRS samples were reconstructed with either fetal fibroblasts (GM10) or adult (21 yr-old donor) fibroblasts within the dermal equivalent. Identical keratinocyte and melanocyte strains were used for the epidermal reconstruction. A drastic increase in pigmentation of the PRS and activation of melanocytes in the presence of fetal fibroblasts as compared to adult fibroblasts, were noted A) macroscopically (Macro), on histological sections stained with Fontana-Masson (FM) and by tyrosinase staining (Tyr) of tissue sections. The hyper-pigmentation observed in fetal versus adult fibroblast condition was quantified by B) a decrease in Luminance value and C) an increase in melanin content. TRP-1 labeling of tissue sections showed that melanocytes were correctly located at the basal layer in both conditions (A). TRP-1 staining of epidermal sheets revealed an increase in melanocyte numbers in the presence of fetal fibroblasts (A) which was confirmed by image analysis (D). CD13 staining revealed no change in fibroblast morphology or density (A). Values are expressed as the mean +/- SD calculated for 4 different samples in 2 independent experiments and analyzed using the two-tailed unpaired Student's t-test, *** p<0.001. Magnifications (A): FM, TYR, and TRP-1/section = x400, CD13/section = x200, TRP1/sheet = x50.
Figure 2 Comparison of the effect of fibroblasts from fetal versus adult origin on reconstructed skin pigmentation. PRS samples were reconstructed with either fetal fibroblasts (GM10) or adult (21 yr-old donor) fibroblasts within the dermal equivalent. Identical keratinocyte and melanocyte strains were used for the epidermal reconstruction. A drastic increase in pigmentation of the PRS and activation of melanocytes in the presence of fetal fibroblasts as compared to adult fibroblasts, were noted A) macroscopically (Macro), on histological sections stained with Fontana-Masson (FM) and by tyrosinase staining (Tyr) of tissue sections. The hyper-pigmentation observed in fetal versus adult fibroblast condition was quantified by B) a decrease in Luminance value and C) an increase in melanin content. TRP-1 labeling of tissue sections showed that melanocytes were correctly located at the basal layer in both conditions (A). TRP-1 staining of epidermal sheets revealed an increase in melanocyte numbers in the presence of fetal fibroblasts (A) which was confirmed by image analysis (D). CD13 staining revealed no change in fibroblast morphology or density (A). Values are expressed as the mean +/- SD calculated for 4 different samples in 2 independent experiments and analyzed using the two-tailed unpaired Student's t-test, *** p<0.001. Magnifications (A): FM, TYR, and TRP-1/section = x400, CD13/section = x200, TRP1/sheet = x50.CC BY · PubMed Central
Figure 3 Morphology and senescence-associated β-galactosidase staining of fibroblasts from photo-aged and young skins. Fibroblasts isolated from photo-aged skin (PAF) and from young unexposed skin (YF) were grown in DMEM + 0 % FBS at 10% CO2 and passaged until P4. As shown by A) classical microscopy, B) senescence-associated β-galactosidase (SA-β-gal) staining and C) population doubling times (time required for a two-fold increase in fibroblast number), PAF exhibited a flattened and enlarged cellular body, a reduced growth rate and an increase in SA-β-galactosidase activity, thus revealing a senescent phenotype. Values are expressed as the mean +/- SD calculated in 3 independent experiments and analyzed using the two-tailed unpaired Student's t-test; *** p<0.001. A and B magnification = x200.
Figure 3 Morphology and senescence-associated β-galactosidase staining of fibroblasts from photo-aged and young skins. Fibroblasts isolated from photo-aged skin (PAF) and from young unexposed skin (YF) were grown in DMEM + 0 % FBS at 10% CO2 and passaged until P4. As shown by A) classical microscopy, B) senescence-associated β-galactosidase (SA-β-gal) staining and C) population doubling times (time required for a two-fold increase in fibroblast number), PAF exhibited a flattened and enlarged cellular body, a reduced growth rate and an increase in SA-β-galactosidase activity, thus revealing a senescent phenotype. Values are expressed as the mean +/- SD calculated in 3 independent experiments and analyzed using the two-tailed unpaired Student's t-test; *** p<0.001. A and B magnification = x200.CC BY · PubMed Central
Figure 4 Reconstruction of pigmented skin with fibroblasts from photo-aged or young skin. Pigmented skins reconstructed with a dermal equivalent containing either photo-aged fibroblasts (PAF) or young fibroblasts (YF) were analyzed after 14 days of emersion. The same keratinocyte and melanocyte strains were used for epidermal reconstruction. In both photo-aged and young fibroblast conditions, HES colorations showed that the histological quality of the epidermis was correct (A). TRP-1 (red) and CD13 (green) co-staining of skin sections indicated the correct location of melanocytes in the basal layer and the presence of fibroblasts within the dermal equivalent (B) and Dopa reaction (C) and TRP1 staining (D) of epidermal sheets revealed the correct morphology of melanocytes in both conditions. E) Graph reporting the number of melanocytes as estimated by counting TRP1-positive cells/mm2 epidermal surface shows no significant difference between the number of melanocytes in the photo-aged versus young fibroblast condition. Values are expressed as the mean +/- SD calculated for 6 samples and analyzed using the two-tailed unpaired Student's t-test; NS: non-significant. Magnification: A, B = x 400, C = x200, D = x50.
Figure 4 Reconstruction of pigmented skin with fibroblasts from photo-aged or young skin. Pigmented skins reconstructed with a dermal equivalent containing either photo-aged fibroblasts (PAF) or young fibroblasts (YF) were analyzed after 14 days of emersion. The same keratinocyte and melanocyte strains were used for epidermal reconstruction. In both photo-aged and young fibroblast conditions, HES colorations showed that the histological quality of the epidermis was correct (A). TRP-1 (red) and CD13 (green) co-staining of skin sections indicated the correct location of melanocytes in the basal layer and the presence of fibroblasts within the dermal equivalent (B) and Dopa reaction (C) and TRP1 staining (D) of epidermal sheets revealed the correct morphology of melanocytes in both conditions. E) Graph reporting the number of melanocytes as estimated by counting TRP1-positive cells/mm2 epidermal surface shows no significant difference between the number of melanocytes in the photo-aged versus young fibroblast condition. Values are expressed as the mean +/- SD calculated for 6 samples and analyzed using the two-tailed unpaired Student's t-test; NS: non-significant. Magnification: A, B = x 400, C = x200, D = x50.CC BY · PubMed Central
Figure 5 Pigmentation levels in skin reconstructed with natural photo-aged fibroblasts versus young fibroblasts. Pigmentation in the skin reconstructed with either natural photo-aged fibroblasts (PAF) or young fibroblasts (YF) was analyzed macroscopically and on histological sections stained with Fontana Masson (A) and quantified by Luminance measurements (L*) (B) and melanin quantification (C) after 14 days of emersion. In the presence of the three PAF strains versus the three YF conditions, a significant increase in pigmentation was observed as shown by i) an increase in the concentration of melanin granules as measured by image analysis on histological sections, and ii) the darkening of the skin samples which correlates with a decrease in Luminance. Values are expressed as a mean +/- SD calculated for 3 samples in 3 independent experiments and analyzed using the two-tailed unpaired Student's t-test; *** p<0.001. Magnification: A = x400.
Figure 5 Pigmentation levels in skin reconstructed with natural photo-aged fibroblasts versus young fibroblasts. Pigmentation in the skin reconstructed with either natural photo-aged fibroblasts (PAF) or young fibroblasts (YF) was analyzed macroscopically and on histological sections stained with Fontana Masson (A) and quantified by Luminance measurements (L*) (B) and melanin quantification (C) after 14 days of emersion. In the presence of the three PAF strains versus the three YF conditions, a significant increase in pigmentation was observed as shown by i) an increase in the concentration of melanin granules as measured by image analysis on histological sections, and ii) the darkening of the skin samples which correlates with a decrease in Luminance. Values are expressed as a mean +/- SD calculated for 3 samples in 3 independent experiments and analyzed using the two-tailed unpaired Student's t-test; *** p<0.001. Magnification: A = x400.CC BY · PubMed Central
Figure 6 Effect of fibroblasts from photo-aged versus young skin on the expression level of melanogenic proteins in reconstructed skin. Analysis of melanocyte location and activity in the reconstructed skin samples containing either photo-aged fibroblasts (PAF) or young fibroblasts (YF) was analyzed by immunostaining on histological sections using TRP-1 and Tyrosinase antibodies (A) and by measuring expression levels of melanogenic genes by RT-qPCR (B). Immunostaining of tissue sections showed the correct location of melanocytes in the basal layer and increased labeling of Tyrosinase in PAF conditions as compared to YF samples. The analysis of expression levels of genes involved in melanogenesis in the epidermis revealed the increase in mRNA levels of Silver, Tyrosinase, TRP-1 and DCT (TRP-2) in the PAF-containing model as compared to the YF condition confirming that melanocytes were stimulated when photo-aged fibroblasts were present in dermal equivalent. Values are expressed as a mean +/- SD calculated for 3 samples in one experiment and analyzed using the two-tailed unpaired Student's t-test; ** p<0.01, *** p<0.001. Similar results were obtained in three independent experiments. Magnification: A = x400.
Figure 6 Effect of fibroblasts from photo-aged versus young skin on the expression level of melanogenic proteins in reconstructed skin. Analysis of melanocyte location and activity in the reconstructed skin samples containing either photo-aged fibroblasts (PAF) or young fibroblasts (YF) was analyzed by immunostaining on histological sections using TRP-1 and Tyrosinase antibodies (A) and by measuring expression levels of melanogenic genes by RT-qPCR (B). Immunostaining of tissue sections showed the correct location of melanocytes in the basal layer and increased labeling of Tyrosinase in PAF conditions as compared to YF samples. The analysis of expression levels of genes involved in melanogenesis in the epidermis revealed the increase in mRNA levels of Silver, Tyrosinase, TRP-1 and DCT (TRP-2) in the PAF-containing model as compared to the YF condition confirming that melanocytes were stimulated when photo-aged fibroblasts were present in dermal equivalent. Values are expressed as a mean +/- SD calculated for 3 samples in one experiment and analyzed using the two-tailed unpaired Student's t-test; ** p<0.01, *** p<0.001. Similar results were obtained in three independent experiments. Magnification: A = x400.CC BY · PubMed Central

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 64 references

Research works printed but not linked: 4

  1. Lavker R.M. (1995) Cutaneous aging: chronologic versus photoaging. in: Gilchrest B.A. (ed): Photodamage. Cambridge USA, Blackwell Science Inc, pp 123–135.
  2. SJ Hedley, 2002, Fibroblasts play a regulatory role in the control of pigmentation in reconstructed human skin from skin types I and II, Pigment Cell Res, 15, 49, 10.1034/j.1600-0749.2002.00067.x · DOI
  3. G Imokawa, 1998, Biological characterization of human fibroblast-derived mitogenic factors for human melanocytes, Biochem J 330 (Pt, 3), 1235, 10.1042/bj3301235 · DOI
  4. D Kovacs, 2010, Role of fibroblast-derived growth factors in regulating hyperpigmentation of solar lentigo, Br J Dermatol, 163, 1020, 10.1111/j.1365-2133.2010.09946.x · DOI

Cited by

Sorted by citations

Related

See all

Metadata

Authors
Duval, C., Cohen, C., Chagnoleau, C., Flouret, V., Bourreau, E., & Bernerd, F.
Affiliations
L'Oréal (France) (FR)
Corresponding author
Duval, Christine
ORCID
Venue
PLoS ONE
Publisher
Public Library of Science (PLoS)
Published
2014-12-09
Volume
9
Issue
12
Pages
e114182
ISSN
1932-6203 (ISSN-L)
DOI
10.1371/journal.pone.0114182
PMC
PMC4260844
OpenAlex
W1972398615
Cited by
114
References
64
Citations by year
2026: 6 · 2025: 8 · 2024: 13 · 2023: 6 · 2022: 8 · 2021: 13 · 2020: 8 · 2019: 12 · 2018: 19 · 2017: 12 · 2016: 7 · 2015: 2
FWCI
3.49
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Figshare (submittedVersion, cc-by)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
  • Health Sciences › Medicine › Dermatology › Dermatologic Treatments and Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Melanoma and MAPK Pathways
Keywords
dermal fibroblasts, skin pigmentation, reconstructed skin model, photoaging, fibroblast senescence, melanin content, melanocyte proliferation, hyperpigmentation, epidermis
MeSH terms
Adult, Animals, Fibroblasts, Humans, Light, Melanocytes, Skin, Skin Pigmentation, Keratinocytes, 3T3 Cells, Cellular Senescence, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review