CCTop: An Intuitive, Flexible and Reliable CRISPR/Cas9 Target Prediction Tool

Fiji: an open-source platform for biological-image analysis

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Multiplex Genome Engineering Using CRISPR/Cas Systems

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The Human Genome Browser at UCSC

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RNA-Guided Human Genome Engineering via Cas9

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DNA targeting specificity of RNA-guided Cas9 nucleases

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Efficient genome editing in zebrafish using a CRISPR-Cas system

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Genome editing with engineered zinc finger nucleases

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A TALE nuclease architecture for efficient genome editing

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A Method for In Situ Reverse Genetic Analysis of Proteins Involved mtDNA Replication

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FLOWERING REPRESSOR AAA + ATPase 1 is a novel regulator of perennial flowering in Arabis alpina

de la Torre, N. V., Vayssières, A., Obeng‐Hinneh, E., Neumann, U., Zhou, Y., Lázaro, A. M. P., Roggen, A., Sun, H., Stolze, S. C., Nakagami, H., et al. (2022) · New Phytologist · doi:10.1111/nph.18374 · Cited by 13

Modular control of vertebrate axis segmentation in time and space

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Mitochondrial complex I deficiency leads to the retardation of early embryonic development in Ndufs4 knockout mice

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PAVOOC: designing CRISPR sgRNAs using 3D protein structures and functional domain annotations

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Off-target effects of CRISPRa on interleukin-6 expression

Soubeyrand, S., Lau, P., Peters, V., & McPherson, R. (2019) · PLoS ONE · doi:10.1371/journal.pone.0224113 · Cited by 13

Testes of DAZL null neonatal sheep lack prospermatogonia but maintain normal somatic cell morphology and marker expression

McLean, Z. L., Appleby, S. J., Wei, J., Snell, R. G., & Oback, B. J. (2020) · Molecular Reproduction and Development · doi:10.1002/mrd.23443 · Cited by 13

Integrated design, execution, and analysis of arrayed and pooled CRISPR genome editing experimentsPreprint

Canver, M. C., Haeussler, M., Bauer, D. E., Orkin, S. H., Sanjana, N. E., Shalem, O., Yuan, G., Zhang, F., Concordet, J., & Pinello, L. (2017) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/125245 · Cited by 13

DENT-seq for genome-wide strand-specific identification of DNA single-strand break sites with single-nucleotide resolutionPreprint

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Endothelial Serotonin Receptor 1B Acts as a Mechanosensor to Drive Atherosclerosis

Jiang, M., Ding, H., Huang, Y., Lau, C. W., Guo, Y., Luo, J., Shih, Y., Xia, Y. Y., Yao, X., Chiu, J., et al. (2025) · Circulation Research · doi:10.1161/circresaha.124.325453 · Cited by 12

AI-driven advances in plant biotechnology: sharpening the edge of plant tissue culture and genome editing

Narra, M., Ray, A., Polley, B., Yang, H., & Bhowmik, P. (2025) · Frontiers in Plant Science · doi:10.3389/fpls.2025.1718810 · Cited by 16

Monitoring biological effects of somatic cell genome editing

Freedman, B., Bulte, J. W. M., Conklin, B. R., Judge, L. M., Dwinell, M. R., Geurts, A. M., Sitton, M. J., Mahajan, V., Kiani, S., Gersbach, C. A., et al. (2026) · Nature Reviews Genetics · doi:10.1038/s41576-025-00916-0 · Cited by 13

Overlapping expression patterns and functions of three paralogous P5B ATPases in Caenorhabditis elegans

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The role of kinetochore dynein in checkpoint silencing is restricted to disassembly of the corona

Ide, A. H., DeLuca, K. F., Wiggan, O., Markus, S. M., & DeLuca, J. G. (2023) · Molecular Biology of the Cell · doi:10.1091/mbc.e23-04-0130 · Cited by 13

Epigenetic-focused CRISPR/Cas9 screen identifies (absent, small, or homeotic)2-like protein (ASH2L) as a regulator of glioblastoma cell survival

Ozyerli‐Goknar, E., Kala, E. Y., Aksu, A. C., Bulut, İ., Cingöz, A., Nizamuddin, S., Biniossek, M. L., Seker-Polat, F., Morova, T., Aztekin, C., et al. (2023) · Cell Communication and Signaling · doi:10.1186/s12964-023-01335-6 · Cited by 12

A mutation in the essential and widely conserved DAMAGED DNA BINDING1‐Cullin4 ASSOCIATED FACTOR gene OZS3 causes hypersensitivity to zinc excess, cold and UV stress in Arabidopsis thaliana

Weber, M., Beyene, B., Nagler, N., Herfert, J., Schempp, S., Klecker, M., & Clemens, S. (2020) · The Plant Journal · doi:10.1111/tpj.14779 · Cited by 12

Transcriptome Profiling of Osteoblasts in a Medaka (Oryzias latipes) Osteoporosis Model Identifies Mmp13b as Crucial for Osteoclast Activation

Liu, R., Imangali, N., Ethiraj, L. P., Carney, T. J., & Winkler, C. (2022) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2022.775512 · Cited by 11

Liver X receptor-agonist treatment rescues degeneration in a Drosophila model of hereditary spastic paraplegia

Byrne, D. J., Garcia-Pardo, M. E., Cole, N. B., Batnasan, B., Heneghan, S., Sohail, A., Blackstone, C. D., & O’Sullivan, N. C. (2022) · Acta Neuropathologica Communications · doi:10.1186/s40478-022-01343-6 · Cited by 13

In vivo identification and validation of novel potential predictors for human cardiovascular diseases

Hammouda, O. T., Wu, M. Y., Kaul, V., Gierten, J., Thumberger, T., & Wittbrodt, J. (2021) · PLoS ONE · doi:10.1371/journal.pone.0261572 · Cited by 11

Epigenetic Editing in Prostate Cancer: Challenges and Opportunities

Pacheco, M. B., Camilo, V., Henrique, R., & Jerónimo, C. (2021) · Epigenetics · doi:10.1080/15592294.2021.1939477 · Cited by 12

TTC30A and TTC30B Redundancy Protects IFT Complex B Integrity and Its Pivotal Role in Ciliogenesis

Hoffmann, F., Bolz, S., Junger, K., Klose, F., Schubert, T., Woerz, F., Boldt, K., Ueffing, M., & Beyer, T. (2022) · Genes · doi:10.3390/genes13071191 · Cited by 11

CRaTER enrichment for on-target gene editing enables generation of variant libraries in hiPSCs

Friedman, C. E., Fayer, S., Pendyala, S., Chien, W., Loiben, A. M., Tran, L. N., Chao, L. S., Mckinstry, A., Ahmed, D., Karbassi, E., et al. (2023) · Journal of Molecular and Cellular Cardiology · doi:10.1016/j.yjmcc.2023.03.017 · Cited by 11

MOZ/ENL complex is a recruiting factor of leukemic AF10 fusion proteins

Komata, Y., Kanai, A., Maeda, T., Inaba, T., & Yokoyama, A. (2023) · Nature Communications · doi:10.1038/s41467-023-37712-5 · Cited by 10

A novel rat model of Dravet syndrome recapitulates clinical hallmarks

Li, M., Yang, L., Qian, W., Ray, S., Lu, Z., Liu, T., Zou, Y., Naumann, R. K., & Wang, H. (2023) · Neurobiology of Disease · doi:10.1016/j.nbd.2023.106193 · Cited by 13

Advances in bread wheat production through CRISPR/Cas9 technology: a comprehensive review of quality and other aspects

Yiğider, E., Taşpınar, M. S., & Ağar, G. (2023) · Planta · doi:10.1007/s00425-023-04199-9 · Cited by 14

Convolution neural network model for predicting single guide RNA efficiency in CRISPR/Cas9 system

Shrawgi, H., & Sisodia, D. S. (2019) · Chemometrics and Intelligent Laboratory Systems · doi:10.1016/j.chemolab.2019.04.008 · Cited by 12

Pcdh18a regulates endocytosis of E-cadherin during axial mesoderm development in zebrafish

Bösze, B., Mattes, B., Sinner, C., Stricker, K., Gourain, V., Thumberger, T., Tlili, S., Weber, S., Wittbrodt, J., Saunders, T. E., et al. (2020) · Histochemistry and Cell Biology · doi:10.1007/s00418-020-01887-5 · Cited by 12

How Crisp is CRISPR? CRISPR-Cas-mediated crop improvement with special focus on nutritional traits

Kaul, T., Sony, S. K., Raman, N. M., Eswaran, M., Verma, R., Prakash, A. T., Bharti, J., Motelb, K. F. A., & Kaul, R. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-818581-0.00011-5 · Cited by 12

EDN1-AS, A Novel Long Non-coding RNA Regulating Endothelin-1 in Human Proximal Tubule Cells

Douma, L. G., Solocinski, K., Masten, S., Barral, D., Barilovits, S. J., Jeffers, L. A., Alder, K. D., Patel, R., Wingo, C. S., Brown, K. D., et al. (2020) · Frontiers in Physiology · doi:10.3389/fphys.2020.00209 · Cited by 12

A Practical Guide to Genome Editing Using Targeted Nuclease Technologies

Porter, S. N., Levine, R. M., & Pruett‐Miller, S. M. (2019) · Comprehensive physiology · doi:10.1002/cphy.c180022 · Cited by 18

Selection constraints of plant adaptation can be relaxed by gene editingPreprint

Ruffley, M., Lutz, U. C., Leventhal, L., Hateley, S. L., Yuan, W., Keck, J., Rhee, S. Y., Weigel, D., & Expósito‐Alonso, M. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.10.16.562583 · Cited by 12

Consumer acceptance and valuation of quality‐improved food products derived by genome editing technology. A case study of rice in Vietnam

Nguyen, T. H., Taieb, S. B., Moritaka, M., & Fukuda, S. (2024) · Agribusiness · doi:10.1002/agr.21929 · Cited by 11

The Evolution of Nucleic Acid–Based Diagnosis Methods from the (pre-)CRISPR to CRISPR era and the Associated Machine/Deep Learning Approaches in Relevant RNA Design

Chakraborty, S. S., Dutta, J. R., Ganesan, R., & Mináry, P. (2024) · Methods in molecular biology · doi:10.1007/978-1-0716-4079-1_17 · Cited by 11

Macrophages Switch to an Osteo‐Modulatory Profile Upon RANKL Induction in a Medaka ( Oryzias latipes ) Osteoporosis Model

Phan, Q. T., Liu, R., Tan, W. H., Imangali, N., Cheong, B., Schartl, M., & Winkler, C. (2020) · JBMR Plus · doi:10.1002/jbm4.10409 · Cited by 11

A Living Organism in your CRISPR Toolbox: Caenorhabditis elegans Is a Rapid and Efficient Model for Developing CRISPR-Cas Technologies

Vicencio, J., & Cerón, J. (2021) · The CRISPR Journal · doi:10.1089/crispr.2020.0103 · Cited by 12

Intracellular Accumulation of IFN-λ4 Induces ER Stress and Results in Anti-Cirrhotic but Pro-HCV Effects

Onabajo, O. O., Wang, F., Lee, M., Flórez-Vargas, O., Obajemu, A. A., Tanikawa, C., Vargas, J. M., Liao, S., Song, C., Huang, Y.-H., et al. (2021) · Frontiers in Immunology · doi:10.3389/fimmu.2021.692263 · Cited by 11

Porcine TRIM21 Enhances Porcine Circovirus 2 Infection and Host Immune Responses, But Inhibits Apoptosis of PCV2-Infected Cells

Yang, L., Liu, X., Zhang, L., Li, X., Zhang, X., Niu, G., Ji, W., Chen, S. E., Ouyang, H., & Ren, L. (2022) · Viruses · doi:10.3390/v14010156 · Cited by 11

Multi-Omics Analysis in β-Thalassemia Using an HBB Gene-Knockout Human Erythroid Progenitor Cell Model

Zhou, G., Zhang, H., Lin, A., Wu, Z., Li, T., Zhang, X., Chen, H., & Lu, D. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23052807 · Cited by 11

BRPF1 bridges H3K4me3 and H3K23ac in human embryonic stem cells and is essential to pluripotency

Zhang, C., Lin, H., Zhang, Y., Qi, X., Zhang, J., Zhang, D., Liu, Y., Chen, Q., Zhou, T., Wang, J., et al. (2023) · iScience · doi:10.1016/j.isci.2023.105939 · Cited by 11

Deficiency of the Polycomb Protein RYBP and TET Methylcytosine Oxidases Promotes Extensive CpG Island Hypermethylation and Malignant Transformation

Cui, W., Huang, Z., Jin, S., Johnson, J. S., Lau, K. H., Hostetter, G. H., & Pfeifer, G. P. (2023) · Cancer Research · doi:10.1158/0008-5472.can-23-0269 · Cited by 11

Hierarchical contribution of Argonaute proteins to antiviral protection

Ludman, M., Szalai, G., Janda, T., & Fátyol, K. (2023) · Journal of Experimental Botany · doi:10.1093/jxb/erad327 · Cited by 11

Expression of the novel maternal centrosome assembly factor Wdr8 is required for vertebrate embryonic mitoses

Inoue, D., Stemmer, M., Thumberger, T., Ruppert, T., Bärenz, F., Wittbrodt, J., & Gruß, O. J. (2017) · Nature Communications · doi:10.1038/ncomms14090 · Cited by 11

Cytosine and adenosine base editing in human pluripotent stem cells using transient reporters for editing enrichment

Tekel, S. J., Brookhouser, N., Standage-Beier, K., Wang, X., & Brafman, D. A. (2021) · Nature Protocols · doi:10.1038/s41596-021-00552-y · Cited by 12

New hPSC SOX9 and INS Reporter Cell Lines Facilitate the Observation and Optimization of Differentiation into Insulin-Producing Cells

Dettmer, R., Niwolik, I., Mehmeti, I., Jörns, A., & Naujok, O. (2021) · Stem Cell Reviews and Reports · doi:10.1007/s12015-021-10232-9 · Cited by 11

Vasoactive Intestinal Peptide Indirectly Elicits Pituitary LH Secretion Independent of GnRH in Female Zebrafish

Tanaka, S., Zmora, N., Levavi-Sivan, B., & Zohar, Y. (2022) · Endocrinology · doi:10.1210/endocr/bqab264 · Cited by 11

An eye on light-sheet microscopy

Kromm, D., Thumberger, T., & Wittbrodt, J. (2016) · Methods in cell biology · doi:10.1016/bs.mcb.2016.01.001 · Cited by 11

Swift Large-scale Examination of Directed Genome Editing

Hammouda, O. T., Böttger, F., Wittbrodt, J., & Thumberger, T. (2019) · PLoS ONE · doi:10.1371/journal.pone.0213317 · Cited by 11

CRISPR-Cas system: a precise tool for plant genome editing

Saraswat, P., & Ranjan, R. (2021) · The Nucleus · doi:10.1007/s13237-021-00353-4 · Cited by 10

A comparison of three different delivery methods for achieving CRISPR/Cas9 mediated genome editing in Cichorium intybus L.

Salvagnin, U., Unkel, K., Sprink, T., Bundock, P., Sévenier, R., Bogdanović, M. D., Todorović, S., Cankar, K., Hakkert, J. C., Schijlen, E. G. W. M., et al. (2023) · Frontiers in Plant Science · doi:10.3389/fpls.2023.1111110 · Cited by 11

Efficient DNA knock-in using AAV-mediated delivery with 2-cell embryo CRISPR-Cas9 electroporation

Davis, D. J., McNew, J. F., Maresca-Fichter, H. C., Chen, K., Telugu, B. P. V. L., & Bryda, E. C. (2023) · Frontiers in Genome Editing · doi:10.3389/fgeed.2023.1256451 · Cited by 10

In pursuit of a minimal CHO genome: Establishment of large-scale genome deletions

Jerabek, T., Weiß, L., Fahrion, H., Zeh, N., Raab, N., Lindner, B., Fischer, S., & Otte, K. (2023) · New Biotechnology · doi:10.1016/j.nbt.2023.12.007 · Cited by 10

Comparison of CRISPR-MAD7 and CRISPR-Cas9 for Gene Disruptions in Komagataella phaffii

Smirnov, K., Weiß, F., Hatzl, A.-M., Rieder, L., Olesen, K., Jensen, S., & Glieder, A. (2024) · Journal of Fungi · doi:10.3390/jof10030197 · Cited by 11

Activation of an atypical plant NLR with an N-terminal deletion initiates cell death at the vacuole

Sunil, S., Beeh, S., Stöbbe, E., Fischer, K., Wilhelm, F., Meral, A., Paris, C., Teasdale, L. C., Jiang, Z., Zhang, L., et al. (2024) · EMBO Reports · doi:10.1038/s44319-024-00240-4 · Cited by 9

Epigenetic regulation by polycomb repressive complex 1 promotes cerebral cavernous malformations

Pham, V. C., Rödel, C. J., Valentino, M., Malinverno, M., Paolini, A., Münch, J., Pasquier, C., Onyeogaziri, F. C., Lazovic, B., Girard, R., et al. (2024) · EMBO Molecular Medicine · doi:10.1038/s44321-024-00152-9 · Cited by 10

CRISPR-MFH: A Lightweight Hybrid Deep Learning Framework with Multi-Feature Encoding for Improved CRISPR-Cas9 Off-Target Prediction

Zheng, Y., Zou, Q., Li, J., & Yang, Y. (2025) · Genes · doi:10.3390/genes16040387 · Cited by 11

Neuronal APOE4-induced early hippocampal network hyperexcitability in Alzheimer’s disease pathogenesis

Tabuena, D. R., Jang, S.-S., Grone, B. P., Yip, O., Jones, E. A. A., Blumenfeld, J. R., Liang, Z., Mann, R. S., Li, Y., Necula, D., et al. (2026) · Nature Aging · doi:10.1038/s43587-026-01096-0 · Cited by 15

Delivery Methods, Resources and Design Tools in CRISPR/Cas

Aslam, M. A., Hammad, M., Ahmad, A., Altenbuchner, J., & Ali, H. (2021) · Springer eBooks · doi:10.1007/978-981-15-7142-8_3 · Cited by 10

The tomato resistance gene Bs4 suppresses leaf watersoaking phenotypes induced by AvrHah1 , a transcription activator‐like effector from tomato‐pathogenic xanthomonads

Schenstnyi, K., Strauß, A., Dressel, A., Morbitzer, R., Wunderlich, M., Galan, A. G. A., Phan, T., Aguilera, P. D. L. A., Brancato, C., Berendzen, K. W., et al. (2022) · New Phytologist · doi:10.1111/nph.18456 · Cited by 10

Editing efficiencies with Cas9 orthologs, Cas12a endonucleases, and temperature in rice

Illa-Berenguer, E., LaFayette, P. R., & Parrott, W. A. (2023) · Frontiers in Genome Editing · doi:10.3389/fgeed.2023.1074641 · Cited by 10

CRISPR/Cas9-Mediated Disruption of the pcz1 Gene and Its Impact on Growth, Development, and Penicillin Production in Penicillium rubens

Gil-Durán, C., Palma, D., Marcano, Y., Palacios, J. L., Martı́nez, C., Rojas-Aedo, J. F., Levicán, G. J., Vaca, I., & Chávez, R. (2023) · Journal of Fungi · doi:10.3390/jof9101010 · Cited by 10

Repurposing screen identifies novel candidates for broad-spectrum coronavirus antivirals and druggable host targets

Haid, S., Matthaei, A., Winkler, M., Sake, S. M., Gunesch, A. P., Milke, V., Köhler, N. M., Rückert, J., Vièyres, G., Kühl, D., et al. (2024) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01210-23 · Cited by 10

Methods for CRISPR/Cas9 Xenopus tropicalis Tissue-Specific Multiplex Genome Engineering

Naert, T., & Vleminckx, K. (2018) · Methods in molecular biology · doi:10.1007/978-1-4939-8784-9_3 · Cited by 10

Precise Correction of Heterozygous SHOX2 Mutations in hiPSCs Derived from Patients with Atrial Fibrillation via Genome Editing and Sib Selection

Sumer, S., Hoffmann, S., Laue, S., Campbell, B., Rädecke, K., Frajs, V., Clauß, S., Kääb, S., Janssen, J. W. G., Jauch, A. S., et al. (2020) · Stem Cell Reports · doi:10.1016/j.stemcr.2020.08.015 · Cited by 10

SeqCor: correct the effect of guide RNA sequences in clustered regularly interspaced short palindromic repeats/Cas9 screening by machine learning algorithm

Liu, X., Yang, Y., Qiu, Y., Reyad‐ul‐Ferdous, M., Ding, Q., & Wang, Y. (2020) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2020.10.007 · Cited by 10

CROP: a CRISPR/Cas9 guide selection program based on mapping guide variants

Aprilyanto, V., Aditama, R., Tanjung, Z. A., Utomo, C., & Liwang, T. (2021) · Scientific Reports · doi:10.1038/s41598-021-81297-2 · Cited by 10

Vasoactive intestinal peptide indirectly elicits pituitary LH secretion independent of GnRH in female zebrafish

Tanaka, S., Zmora, N., Levavi‐Sivan, B., & Zohar, Y. (2022) · Maryland Shared Open Access Repository (USMAI Consortium) · doi:10.13016/m2ofck-jexr · Cited by 10

A sequential transformation method for validating soybean genome editing by CRISPR/Cas9 system

Trinh, D. D., Le, N. T., Bui, T. P., Le, T. N. T., Nguyen, C. X., Hà, C. H., & Do, P. T. (2022) · Saudi Journal of Biological Sciences · doi:10.1016/j.sjbs.2022.103420 · Cited by 10

Genome Editing of Medaka

Ansai, S., & Kinoshita, M. (2017) · Methods in molecular biology · doi:10.1007/978-1-4939-7128-2_15 · Cited by 10

Nicotiana benthamiana, A Popular Model for Genome Evolution and Plant–Pathogen Interactions

Pombo, M. A., Rosli, H. G., Fernández‐Pozo, N., & Bombarely, A. (2020) · Compendium of plant genomes · doi:10.1007/978-3-030-29493-9_14 · Cited by 10

Computational Protein Engineering Approaches for Effective Design of New Molecules

Dhanjal, J. K., Malik, V., Radhakrishnan, N., Sigar, M., Kumari, A., & Sundar, D. (2018) · Elsevier eBooks · doi:10.1016/b978-0-12-809633-8.20150-7 · Cited by 10

A HIT-trapping strategy for rapid generation of reversible and conditional alleles using a universal donorPreprint

Lu, H., Liu, J., Feng, T., Guo, Z., Yin, Y., Gao, F., Cao, G., Du, X., & Wu, S. (2021) · Genome Research · doi:10.1101/gr.271312.120 · Cited by 10

Improved base editing and functional screening in Leishmania via co-expression of the AsCas12a ultra variant, a T7 RNA polymerase, and a cytosine base editorPreprint

May, N. H., Cao, A., Schmid, A., Link, F., Arias-del-Angel, J. A., Meiser, E., & Beneke, T. (2024) · eLife · doi:10.7554/elife.97437 · Cited by 10

PINE-TREE enables highly efficient genetic modification of human cell lines.

Frisch, C., Kostes, W. W., Galyon, B., Whitman, B., Tekel, S. J., Standage-Beier, K., Srinivasan, G., Wang, X., & Brafman, D. A. (2023) · Molecular Therapy - Nucleic Acids · doi:10.1016/j.omtn.2023.07.007 · Cited by 9

In silico analysis of potential off-target sites to gene editing for Mucopolysaccharidosis type I using the CRISPR/Cas9 system: Implications for population-specific treatments

Carneiro, P., de Freitas, M. V., & Matte, Ú. D. S. (2022) · PLoS ONE · doi:10.1371/journal.pone.0262299 · Cited by 9

CRISPR/Cas9 genetic screens in hepatocellular carcinoma gene discovery

Chiu, C. H. (2023) · Current Research in Biotechnology · doi:10.1016/j.crbiot.2023.100127 · Cited by 10

Interactome Analysis Reveals a Link of the Novel ALMS1-CEP70 Complex to Centrosomal Clusters

Woerz, F., Hoffmann, F., Antony, S., Bolz, S., Jarboui, M. A., Junger, K., Klose, F., Stehle, I. F., Boldt, K., Ueffing, M., et al. (2023) · Molecular & Cellular Proteomics · doi:10.1016/j.mcpro.2023.100701 · Cited by 12

Graded FGF activity patterns distinct cell types within the apical sensory organ of the sea anemone Nematostella vectensis

Sabin, K. Z., Chen, S., Hill, E. M., Weaver, K. J., Yonke, J., Kirkman, M., Redwine, W. B., Klompen, A. M. L., Zhao, X., Guo, F., et al. (2024) · Developmental Biology · doi:10.1016/j.ydbio.2024.02.010 · Cited by 10

Easy‐Curing and pH ‐Regulated CRISPR ‐Cas9 Plasmids for Gene Editing and Plasmid Curing in Lactococcus cremoris

Garay‐Novillo, J. N., Ruiz-Masó, J. A., del Solar, G. H., & Barra, J. L. (2024) · Microbial Biotechnology · doi:10.1111/1751-7915.70060 · Cited by 10

Using a novel panel of drug-resistant triple-negative breast cancer cell lines to identify candidate therapeutic targets and biomarkers

Grimsley, H. E., Antczak, M., Reddin, I. G., Weiler, N., McLaughlin, K.-M., Rothweiler, F., Haas, J., Nist, A., Mernberger, M., Stiewe, T., et al. (2025) · Cancer Letters · doi:10.1016/j.canlet.2025.217754 · Cited by 9

CRISPR–Cas9 screening reveals microproteins regulating adipocyte proliferation and lipid metabolism

Pai, V. J., Shan, H., Donaldson, C. J., Vaughan, J. M., de Souza, E. V., O’Connor, C., Liem, M., Pinto, A. F. M., Diedrich, J. K., & Saghatelian, A. (2025) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2506534122 · Cited by 11

Methods for detecting off-target effects of CRISPR/Cas9

Xu, Y., Zhou, S., Wang, L.-Y., Zhang, R., Li, K., Qian, Z., & Li, X. (2025) · Biotechnology Advances · doi:10.1016/j.biotechadv.2025.108750 · Cited by 11

Comprehensive profiling of CRISPR/dCas9 epigenome editors indicates a complex link between on and off target effects

Kakhki, M. P., Rangani, F., Ewing, E., Cucuzza, C. S., Zheleznyakova, G. Y., Kalomoiri, M., Kenny, L., Raghavan, A., Prakash, C. R., van den Hoeven, G., et al. (2026) · Genome biology · doi:10.1186/s13059-026-03967-6 · Cited by 9

Balancing off-target and on-target considerations for optimized CRISPR-Cas9 knockout library design

Drepanos, L. M., Srikanth, S., Kaplan, E. G., Shah, S. T., Velasco, B. E., Merzouk, S., & Doench, J. G. (2026) · Cell Genomics · doi:10.1016/j.xgen.2026.101190 · Cited by 9

Clustered Regularly Interspaced Short Palindromic Repeats

Chrenek, M. A., Nickerson, J. M., & Boatright, J. H. (2016) · Asia-Pacific Journal of Ophthalmology · doi:10.1097/apo.0000000000000225 · Cited by 9

CRISPR/Cas9‐mediated efficient and precise targeted integration of donor DNA harboring double cleavage sites in Xenopus tropicalis

Mao, C., Zheng, L., Zhou, Y., Wu, H., Xia, J., Liang, C., Guo, X., Peng, W., Zhao, H., Cai, W., et al. (2018) · The FASEB Journal · doi:10.1096/fj.201800093 · Cited by 9

Motif scraper: a cross-platform, open-source tool for identifying degenerate nucleotide motif matches in FASTA files

Roberson, E. D. O. (2018) · Bioinformatics · doi:10.1093/bioinformatics/bty437 · Cited by 9

CRISPR/Cas9 System: An Advanced Approach for the Improvement of Industrially Important Microorganisms

Sarkar, S., Yadav, M., & Kumar, A. A. (2023) · Springer eBooks · doi:10.1007/978-981-99-2816-3_4 · Cited by 9

A JAGN1-associated severe congenital neutropenia zebrafish model revealed an altered G-CSFR signaling and UPR activation

Doll, L., Welte, K., Skokowa, J., & Bajoghli, B. (2024) · Blood Advances · doi:10.1182/bloodadvances.2023011656 · Cited by 9

Generation of a Double KO Mouse by Simultaneous Targeting of the Neighboring Genes Tmem176a and Tmem176b Using CRISPR/Cas9: Key Steps from Design to Genotyping

Lemoine, A., Friec, G. C.-L., Vives, F. L., & Louvet, C. (2016) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2016.04.004 · Cited by 9

CRISPR/Cas-Mediated In Planta Gene Targeting

Schiml, S., Fauser, F., & Puchta, H. (2017) · Methods in molecular biology · doi:10.1007/978-1-4939-7003-2_1 · Cited by 9

Pinpointing recurrent proviral integration sites in new models for latent HIV-1 infection

Lange, U. C., Bialek, J. K., Walther, T., & Hauber, J. (2018) · Virus Research · doi:10.1016/j.virusres.2018.03.007 · Cited by 8

Functional Studies of Transcriptional Cofactors via Microinjection-Mediated Gene Editing in Xenopus

Shibata, Y., Bao, L., Fu, L., Shi, B., & Shi, Y. (2018) · Methods in molecular biology · doi:10.1007/978-1-4939-8831-0_29 · Cited by 9

Genetic Manipulation of MicroRNAs in the Silk Gland of Silkworm, Bombyx Mori

Wang, W., Wang, X., Li, X., Pu, Q., Luo, C., Xu, L., Peng, X., & Liu, S. (2019) · Biological Procedures Online · doi:10.1186/s12575-019-0102-4 · Cited by 9

Genome editing of algal species by CRISPR Cas9 for biofuels

Tanwar, A., & Kumar, S. B. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-818140-9.00015-5 · Cited by 9

Predicting CRISPR-Cas9 Off-target with Self-supervised Neural Networks

Chen, D., Shu, W., & Peng, S. (2020) · IEEE International Conference on Bioinformatics and Biomedicine (BIBM) · doi:10.1109/bibm49941.2020.9313280 · Cited by 9

High-throughput screening and validation of antibodies against synaptic proteins to explore opioid signaling dynamics

Duarte, M. L., Trimbake, N. A., Gupta, A., Tumanut, C., Fan, X., Woods, C., Ram, A., Gomes, I., Bobeck, E. N., Schechtman, D., et al. (2021) · Communications Biology · doi:10.1038/s42003-021-01744-8 · Cited by 9

Targeted Gene Disruption Via CRISPR/Cas9 Ribonucleoprotein Complexes in Fusarium oxysporum

Pokhrel, A., Seo, S., Wang, Q., & Coleman, J. J. (2021) · Methods in molecular biology · doi:10.1007/978-1-0716-1795-3_7 · Cited by 9

Wnt11 acts on dermomyotome cells to guide epaxial myotome morphogenesis

Heilig, A. K., Nakamura, R., Shimada, A., Hashimoto, Y., Nakamura, Y., Wittbrodt, J., Takeda, H., & Kawanishi, T. (2022) · eLife · doi:10.7554/elife.71845 · Cited by 9

CRISPR/Cas9-mediated knock-out of AGXT1 in HepG2 cells as a new in vitro model of Primary Hyperoxaluria Type 1

Gatticchi, L., Grottelli, S., Ambrosini, G., Pampalone, G., Gualtieri, O., Dando, I., Bellezza, I., & Cellini, B. (2022) · Biochimie · doi:10.1016/j.biochi.2022.08.005 · Cited by 9

Metabolomics analysis of antiquitin deficiency in cultured human cells and plasma: Relevance to pyridoxine‐dependent epilepsy

Crowther, L. M., Poms, M., Zandl‐Lang, M., Abela, L., Hartmann, H., Seiler, M., Mathis, D., & Plecko, B. (2022) · Journal of Inherited Metabolic Disease · doi:10.1002/jimd.12569 · Cited by 9

CRISPcut: A novel tool for designing optimal sgRNAs for CRISPR/Cas9 based experiments in human cells

Dhanjal, J. K., Radhakrishnan, N., & Sundar, D. (2018) · Genomics · doi:10.1016/j.ygeno.2018.03.011 · Cited by 12

Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites

Park, J., Bae, S., & Kim, J. (2015) · Bioinformatics · doi:10.1093/bioinformatics/btv537 · Cited by 483

CT-Finder: A Web Service for CRISPR Optimal Target Prediction and Visualization

Zhu, H., Misel, L., Graham, M., Robinson, M. L., & Liang, C. (2016) · Scientific Reports · doi:10.1038/srep25516 · Cited by 38

CasOT: A genome-wide cas9/gRNA off-target searching tool

Xiao, A., Cheng, Z., Kong, L., Zhu, Z., Lin, S., Gao, G., & Zhang, B. (2014) · Bioinformatics · doi:10.1093/bioinformatics/btt764 · Cited by 363

Computational Prediction of CRISPR/Cas9 Target Sites Reveals Potential Off-Target Risks in Human and Mouse

Wang, Q., & Ui‐Tei, K. (2017) · Methods in molecular biology · doi:10.1007/978-1-4939-7128-2_4 · Cited by 11