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Muscle Releases Alpha-Sarcoglycan Positive Extracellular Vesicles Carrying miRNAs in the Bloodstream
Open accessAbstract
In the past few years, skeletal muscle has emerged as an important secretory organ producing soluble factors, called myokines, that exert either autocrine, paracrine or endocrine effects. Moreover, recent studies have shown that muscle releases microRNAs into the bloodstream in response to physical exercise. These microRNAs affect target cells, such as hormones and cytokines. The mechanisms underlying microRNA secretion are poorly characterized at present. Here, we investigated whether muscle tissue releases extracellular vesicles (EVs), which carry microRNAs in the bloodstream under physiological conditions such as physical exercise. Using density gradient separation of plasma from sedentary and physically fit young men we found EVs positive for TSG101 and alpha-sarcoglycan (SGCA), and enriched for miR-206. Cytometric analysis showed that the SGCA+ EVs account for 1-5% of the total and that 60-65% of these EVs were also positive for the exosomal marker CD81. Furthermore, the SGCA-immuno captured sub-population of EVs exhibited higher levels of the miR-206/miR16 ratio compared to total plasma EVs. Finally, a significant positive correlation was found between the aerobic fitness and muscle-specific miRNAs and EV miR-133b and -181a-5p were significantly up-regulated after acute exercise. Thus, our study proposes EVs as a novel means of muscle communication potentially involved in muscle remodeling and homeostasis.
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“These ELVs were also positive for CD81, TSG101 and miR-206”
From Skeletal Muscle-Released Extracellular Vesicles: State of the Art (2019) · cites this paper as Guescini et al., 2015
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“ELVs containing alpha-sarcoglycan (SGCA) a component of the dystrophin-glycoprotein complex involved in the stability of muscle fiber membranes, were found in human plasma”
From Skeletal Muscle-Released Extracellular Vesicles: State of the Art (2019) · cites this paper as Guescini et al., 2015
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Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302 · cited 1×
“After centrifugation at 18,000 g, protein concentration was determined by the Bradford assay [35].”
MethodAnalysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
Livak, K. J., & Schmittgen, T. D. (2001) · Methods · doi:10.1006/meth.2001.1262 · Cited by 180,860 · cited 1×
“Quantification was performed according to the ΔCq method [34], miR-16 or the exogenously added spike-in cel-miR-39 were used as a reference.”
MethodValadi, H., Ekström, K., Bossios, A., Sjöstrand, M., Lee, J. J., & Lötvall, J. O. (2007) · Nature Cell Biology · doi:10.1038/ncb1596 · Cited by 12,834 · cited 1×
“Numerous studies indicate that circulating miRNAs may be protected from degradation by several complementary mechanisms, including their incorporation into secreted membrane vesicles such as microvesicles, exosomes or apoptotic bodies [16–18], or via the formation of RNA-binding protein complexes [19].”
Background(2014) · Medical Entomology and Zoology · Cited by 9,400
Skog, J., Würdinger, T., van Rijn, S., Meijer, D. H., Gainche, L., Curry, W. T., Carter, B. S., Krichevsky, A. M., & Breakefield, X. O. (2008) · Nature Cell Biology · doi:10.1038/ncb1800 · Cited by 5,001
Mutation of the mouse klotho gene leads to a syndrome resembling ageing
Kuro-o, M., Matsumura, Y., Aizawa, H., Kawaguchi, H., Suga, T., Utsugi, T., Ohyama, Y., Kurabayashi, M., Kaname, T., Kume, E., et al. (1997) · Nature · doi:10.1038/36285 · Cited by 3,837
Arroyo, J. D., Chevillet, J. R., Kroh, E. M., Ruf, I. K., Pritchard, C. C., Gibson, D. F., Mitchell, P. S., Bennett, C. F., Pogosova-Agadjanyan, E. L., Stirewalt, D. L., et al. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1019055108 · Cited by 3,294 · cited 1×
“Numerous studies indicate that circulating miRNAs may be protected from degradation by several complementary mechanisms, including their incorporation into secreted membrane vesicles such as microvesicles, exosomes or apoptotic bodies [16–18], or via the formation of RNA-binding protein complexes [19].”
BackgroundMuscles, exercise and obesity: skeletal muscle as a secretory organ
Pedersen, B. K., & Febbraio, M. A. (2012) · Nature Reviews Endocrinology · doi:10.1038/nrendo.2012.49 · Cited by 2,909 · cited 1×
“Therefore, the discovery that contracting muscle is a myokine-producing organ has opened up a whole new field of research on skeletal muscle [6].”
BackgroundMicroRNAs are transported in plasma and delivered to recipient cells by high-density lipoproteins
Vickers, K. C., Palmisano, B. T., Shoucri, B. M., Shamburek, R. D., & Remaley, A. T. (2011) · Nature Cell Biology · doi:10.1038/ncb2210 · Cited by 2,741 · cited 2×
“In the extracellular environment, miRNAs could be stabilized by EVs (such as exosomes, microvesicles or apoptotic bodies), HDL/LDLs, or protected by RNA-binding proteins (reviewed in [14,45]).”
BackgroundExosomes: Extracellular organelles important in intercellular communication
Mathivanan, S., Ji, H. L., & Simpson, R. J. (2010) · Journal of Proteomics · doi:10.1016/j.jprot.2010.06.006 · Cited by 2,581 · cited 1×
“11 g/mL, as previously described [37].”
Muscle as an Endocrine Organ: Focus on Muscle-Derived Interleukin-6
Pedersen, B. K., & Febbraio, M. A. (2008) · Physiological Reviews · doi:10.1152/physrev.90100.2007 · Cited by 2,388
Membrane vesicles, current state-of-the-art: emerging role of extracellular vesicles
György, B., Szabó, T. G., Pásztói, M., Pál, Z., Misják, P., Aradi, B., László, V., Pállinger, É., Pap, E., Kittel, Á., et al. (2011) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-011-0689-3 · Cited by 2,160
Secretory Mechanisms and Intercellular Transfer of MicroRNAs in Living Cells
Kosaka, N., Iguchi, H., Yoshioka, Y., Takeshita, F., Matsuki, Y., & Ochiya, T. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.107821 · Cited by 1,934 · cited 1×
“Recent studies have suggested that circulating miRNAs could mediate gene expression in target cells such as hormones and cytokines [13–15].”
BackgroundShedding microvesicles: artefacts no more
Cocucci, E., Racchetti, G., & Meldolesi, J. (2009) · Trends in Cell Biology · doi:10.1016/j.tcb.2008.11.003 · Cited by 1,742 · cited 1×
“These vesicles carry signals either in their limiting membrane or in their interior lumen and can be secreted from various cell types to the extracellular environment both constitutively and in a regulated manner [20,21].”
BackgroundCriteria for maximal oxygen uptake: review and commentary
HOWLEY, E. T., BASSETT, D. R., & WELCH, H. G. (1995) · Medicine & Science in Sports & Exercise · doi:10.1249/00005768-199509000-00009 · Cited by 1,656 · cited 1×
“[31], subjects were considered to have reached their maximal oxygen consumption (VO2max) when one or more of the following criteria were met: I) leveling off of oxygen consumption with increasing workload; II) respiratory exchange ratio values>1.”
BackgroundNon-invasive analysis of acquired resistance to cancer therapy by sequencing of plasma DNA
Murtaza, M., Dawson, S.-J., Tsui, D. W. Y., Gale, D., Forshew, T., Piskorz, A. M., Parkinson, C., Chin, S.-F., Kingsbury, Z., Wong, A. S. C., et al. (2013) · Nature · doi:10.1038/nature12065 · Cited by 1,624 · cited 1×
“Indeed, a novel concept for biomarkers, called “liquid biopsy,” has been proposed [57,58].”
BackgroundDetection of microRNA Expression in Human Peripheral Blood Microvesicles
Hunter, M. P., Ismail, N., Zhang, X., Aguda, B. D., Lee, E. J., Yu, L., Xiao, T., Schafer, J., Lee, M. T., Schmittgen, T. D., et al. (2008) · PLoS ONE · doi:10.1371/journal.pone.0003694 · Cited by 1,476
Delivery of MicroRNA-126 by Apoptotic Bodies Induces CXCL12-Dependent Vascular Protection
Zernecke, A., Bidzhekov, K., Noels, H., Shagdarsuren, E., Gan, L., Denecke, B., Hristov, M., Koeppel, T. A., Nazari-Jahantigh, M., Lutgens, E., et al. (2009) · Science Signaling · doi:10.1126/scisignal.2000610 · Cited by 1,368
Forshew, T., Murtaza, M., Parkinson, C., Gale, D. G., Tsui, D. W. Y., Kaper, F., Dawson, S., Piskorz, A., Jimenez‐Liñan, M., Bentley, D., et al. (2012) · Science Translational Medicine · doi:10.1126/scitranslmed.3003726 · Cited by 1,308 · cited 1×
“Indeed, a novel concept for biomarkers, called “liquid biopsy,” has been proposed [57,58].”
BackgroundExosomal MicroRNA: A Diagnostic Marker for Lung Cancer
Rabinowits, G., Gerçel-Taylor, Ç., Day, J. M., Taylor, D. D., & Kloecker, G. H. (2009) · Clinical Lung Cancer · doi:10.3816/clc.2009.n.006 · Cited by 1,290 · cited 1×
“Although an association between circulating miRNA and EVs such as microvesicles, exosomes or apoptotic bodies has been demonstrated [26,43,44], the mechanisms through which muscle releases miRNAs in plasma have yet to be determined.”
BackgroundSecreted Monocytic miR-150 Enhances Targeted Endothelial Cell Migration
Zhang, Y., Liu, D., Chen, X., Li, J., Li, L., Bian, Z., Sun, F., Lu, J., Yin, Y., Cai, X. F., et al. (2010) · Molecular Cell · doi:10.1016/j.molcel.2010.06.010 · Cited by 1,219 · cited 1×
“On the other hand, there is a great deal of evidence demonstrating the internalization and regulation activity of miRNAs contained in EVs [40,41].”
BackgroundTauro, B. J., Greening, D. W., Mathias, R. A., Ji, H. L., Mathivanan, S., Scott, A. M., & Simpson, R. J. (2012) · Methods · doi:10.1016/j.ymeth.2012.01.002 · Cited by 1,214 · cited 1×
“The density of each fraction was determined by absorbance at 244 nm of 1:10,000 diluted fractions as reported [32].”
MethodNon-Invasive Analysis of Acquired Resistance to Cancer Therapy By Sequencing of Plasma Dna
(2014) · Annals of Oncology · doi:10.1093/annonc/mdu312.5 · Cited by 1,174
ExoCarta 2012: database of exosomal proteins, RNA and lipids
Mathivanan, S., Fahner, C. J., Reid, G. E., & Simpson, R. J. (2011) · Nucleic Acids Research · doi:10.1093/nar/gkr828 · Cited by 1,096 · cited 1×
“Biochemical and biological techniques have been used to identify the protein, mRNA, miRNA, DNA and lipid contents of EVs [23–25].”
Export of microRNAs and microRNA-protective protein by mammalian cells
Wang, K., Zhang, S., Weber, J., Baxter, D., & Galas, D. J. (2010) · Nucleic Acids Research · doi:10.1093/nar/gkq601 · Cited by 1,002 · cited 1×
“Recent studies have suggested that circulating miRNAs could mediate gene expression in target cells such as hormones and cytokines [13–15].”
BackgroundXin, H., Li, Y., Liu, Z., Wang, X., Shang, X., Cui, Y., Zhang, Z. G., & Chopp, M. (2013) · Stem Cells · doi:10.1002/stem.1409 · Cited by 731 · cited 1×
“On the other hand, there is a great deal of evidence demonstrating the internalization and regulation activity of miRNAs contained in EVs [40,41].”
BackgroundSecreted microRNAs: a new form of intercellular communication
Chen, X., Liang, H., Zhang, J., Zen, K., & Zhang, C.-Y. (2012) · Trends in Cell Biology · doi:10.1016/j.tcb.2011.12.001 · Cited by 690 · cited 1×
“It has been widely demonstrated that EVs can be released by many different types of cells and operate as safe containers mediating inter-cellular communication [22].”
BackgroundAstrocytes and Glioblastoma cells release exosomes carrying mtDNA
Guescini, M., Genedani, S., Stocchi, V., & Agnati, L. F. (2009) · Journal of Neural Transmission · doi:10.1007/s00702-009-0288-8 · Cited by 662 · cited 1×
“Biochemical and biological techniques have been used to identify the protein, mRNA, miRNA, DNA and lipid contents of EVs [23–25].”
MethodSearching for the exercise factor: is IL-6 a candidate?
Pedersen (2003) · Journal of Muscle Research and Cell Motility · doi:10.1023/a:1026070911202 · Cited by 564 · cited 1×
“[2] demonstrated that the cytokine IL-6 is produced and released by muscle fibers in response to acute aerobic exercise.”
BackgroundMicroRNAs: powerful new regulators of heart disease and provocative therapeutic targets
van Rooij, E., & Olson, E. N. (2007) · Journal of Clinical Investigation · doi:10.1172/jci33099 · Cited by 554
Extracellular miRNAs: the mystery of their origin and function
Turchinovich, A. A., Weiz, L., & Burwinkel, B. (2012) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2012.08.003 · Cited by 534 · cited 1×
“In the extracellular environment, miRNAs could be stabilized by EVs (such as exosomes, microvesicles or apoptotic bodies), HDL/LDLs, or protected by RNA-binding proteins (reviewed in [14,45]).”
BackgroundProteomics, transcriptomics and lipidomics of exosomes and ectosomes
Choi, D., Kim, D., Kim, Y., & Gho, Y. S. (2013) · PROTEOMICS · doi:10.1002/pmic.201200329 · Cited by 528 · cited 1×
“Biochemical and biological techniques have been used to identify the protein, mRNA, miRNA, DNA and lipid contents of EVs [23–25].”
MethodCirculating microRNAs: Association with disease and potential use as biomarkers
Reid, G., Kirschner, M. B., & van Zandwijk, N. (2010) · Critical Reviews in Oncology/Hematology · doi:10.1016/j.critrevonc.2010.11.004 · Cited by 472 · cited 1×
“Recent studies have demonstrated that muscle miRNAs (MyomiRs) are present in the serum and plasma in a variety of physiological and pathological conditions [26,42].”
BackgroundSenescence-Associated Exosome Release from Human Prostate Cancer Cells
Lehmann, B. D., Paine, M. S., Brooks, A. M., McCubrey, J. A., Renegar, R. H., Wang, R., & Terrian, D. M. (2008) · Cancer Research · doi:10.1158/0008-5472.can-07-6538 · Cited by 464 · cited 1×
“Although an association between circulating miRNA and EVs such as microvesicles, exosomes or apoptotic bodies has been demonstrated [26,43,44], the mechanisms through which muscle releases miRNAs in plasma have yet to be determined.”
BackgroundBaggish, A. L., Hale, A., Weiner, R. B., Lewis, G. D., Systrom, D., Wang, F., Wang, T. J., & Chan, S. Y. (2011) · The Journal of Physiology · doi:10.1113/jphysiol.2011.213363 · Cited by 431 · cited 4×
“[7], using other exercise protocols, researchers have shown the modulation of specific myomiRs in blood.”
BackgroundMethodInfluentialItinerant exosomes: emerging roles in cell and tissue polarity
Lakkaraju, A., & Rodriguez-Boulan, E. J. (2008) · Trends in Cell Biology · doi:10.1016/j.tcb.2008.03.002 · Cited by 408 · cited 1×
“These vesicles carry signals either in their limiting membrane or in their interior lumen and can be secreted from various cell types to the extracellular environment both constitutively and in a regulated manner [20,21].”
BackgroundThe miRNA Plasma Signature in Response to Acute Aerobic Exercise and Endurance Training
Nielsen, S. J., Akerstrom, T. C. A., Rinnov, A., Yfanti, C., Schéele, C., Pedersen, B. K., & Laye, M. J. (2014) · PLoS ONE · doi:10.1371/journal.pone.0087308 · Cited by 306 · cited 2×
“These data are in line with recent studies which have shown an increase in circulating MyomiRs in response to acute aerobic exercise [51,52]; however, these studies did not address the question of which type of carrier transports miRNAs.”
BackgroundResultC2C12 myoblasts release micro-vesicles containing mtDNA and proteins involved in signal transduction
Guescini, M., Guidolin, D., Vallorani, L., Casadei, L., Gioacchini, A. M., Tibollo, P., Battistelli, M., Falcieri, E., Battistin, L., Agnati, L. F., et al. (2010) · Experimental Cell Research · doi:10.1016/j.yexcr.2010.04.006 · Cited by 294 · cited 1×
“Our team has already demonstrated that the muscle cell line C2C12 releases exosome-like vesicles in conditioned medium [46].”
BackgroundRussell, A. P., Lamon, S., Boon, H., Wada, S., Güller, I., Brown, E. L., Chibalin, A., Zierath, J. R., Snow, R. J., Stepto, N. K., et al. (2013) · The Journal of Physiology · doi:10.1113/jphysiol.2013.255695 · Cited by 272 · cited 2×
“For example, following a single bout of cycling miR-1, miR-133a, miR-133b, and miR-181a levels were found to be increased in the skeletal muscle tissue of untrained subjects [50]; however, evidence based on plasma miRNAs showed that all muscle-enriched miRNAs (miR-1, miR-133a, miR-133b, miR-206, miR-208b, and miR-499)…”
BackgroundLi, C. C. Y., Eaton, S. A., Young, P. E., Lee, M. C., Shuttleworth, R., Humphreys, D. T., Grau, G. E., Combes, V., Bebawy, M., Gong, J., et al. (2013) · RNA Biology · doi:10.4161/rna.25281 · Cited by 244 · cited 1×
“Indeed, a growing body of evidence demonstrates that EVs can act as intercellular communication devices acting as safe vesicular carriers of molecules such as receptors, transmembrane proteins, kinases, mRNA, miRNA, long noncoding RNA, DNA and lipids [54–56].”
BackgroundRapid upregulation and clearance of distinct circulating microRNAs after prolonged aerobic exercise
Baggish, A. L., Park, J., Min, P., Isaacs, S. K., Parker, B. A., Thompson, P. D., Troyanos, C., d’Hemecourt, P. A., Dyer, S., Thiel, M., et al. (2014) · Journal of Applied Physiology · doi:10.1152/japplphysiol.01141.2013 · Cited by 210 · cited 2×
“Indeed after a marathon, specific miRNAs originating from skeletal muscle (miR-1, miR-133a), cardiac muscle (miR-208a), and inflammatory processes (miR-146a) were robustly up-regulated [52].”
BackgroundResultCirculating microRNA-126 increases after different forms of endurance exercise in healthy adults
Uhlemann, M., Möbius‐Winkler, S., Fikenzer, S., Adam, J., Redlich, M., Möhlenkamp, S., Hilberg, T., Schuler, G. C., & Adams, V. (2012) · European Journal of Preventive Cardiology · doi:10.1177/2047487312467902 · Cited by 205
Forterre, A. V., Jalabert, A., Chikh, K., Pesenti, S., Euthine, V., Granjon, A., Errazuriz, E., Lefai, É., Vidal, H., & Rome, S. (2013) · Cell Cycle · doi:10.4161/cc.26808 · Cited by 191 · cited 1×
“Furthermore, two key studies from separate groups have recently shown that muscle cells release exosomes carrying bio-active miRNAs [47,48].”
BackgroundThe MyomiR Network in Skeletal Muscle Plasticity
McCarthy, J. J. (2011) · Exercise and Sport Sciences Reviews · doi:10.1097/jes.0b013e31821c01e1 · Cited by 190 · cited 1×
“The OptiPrep density fractions were further analyzed for the presence of miR-206, a miRNA specifically expressed in muscle tissues [38].”
BackgroundGuescini, M., Sisti, D., Rocchi, M., Stocchi, L., & Stocchi, V. (2008) · BMC Bioinformatics · doi:10.1186/1471-2105-9-326 · Cited by 182
Muscle-enriched microRNA miR-486 decreases in circulation in response to exercise in young men
Aoi, W., Ichikawa, H., Mune, K., Tanimura, Y., Mizushima, K., Naito, Y., & Yoshikawa, T. (2013) · Frontiers in Physiology · doi:10.3389/fphys.2013.00080 · Cited by 179
Circulating MicroRNAs and Aerobic Fitness – The HUNT-Study
Bye, A., Røsjø, H. R., Aspenes, S. T., Condorelli, G., Omland, T., & Wisløff, U. (2013) · PLoS ONE · doi:10.1371/journal.pone.0057496 · Cited by 166 · cited 1×
“[49] found that miR-21, miR-210, and miR-222 were higher in the low VO2max-group than in the control group.”
BackgroundExtracellular communication via microRNA: lipid particles have a new message
Rayner, K. J., & Hennessy, E. J. (2013) · Journal of Lipid Research · doi:10.1194/jlr.r034991 · Cited by 162
MicroRNA transport: A new way in cell communication
Xu, L., Yang, B., & Ai, J. (2013) · Journal of Cellular Physiology · doi:10.1002/jcp.24344 · Cited by 138 · cited 1×
“In the extracellular environment, miRNAs could be stabilized by EVs (such as exosomes, microvesicles or apoptotic bodies), HDL/LDLs, or protected by RNA-binding proteins (reviewed in [14,45]).”
BackgroundHudson, M. B., Woodworth-Hobbs, M. E., Zheng, B., Rahnert, J. A., Blount, M. A., Gooch, J. L., Searles, C. D., & Price, S. R. (2014) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00266.2013 · Cited by 137 · cited 1×
“Furthermore, two key studies from separate groups have recently shown that muscle cells release exosomes carrying bio-active miRNAs [47,48].”
BackgroundComputational reconstruction of the human skeletal muscle secretome
Bortoluzzi, S., Scannapieco, P., Cestaro, A., Danieli, G. A., & Schiaffino, S. (2005) · Proteins Structure Function and Bioinformatics · doi:10.1002/prot.20803 · Cited by 136 · cited 1×
“other cytokines and several hundred proteins [3,4].”
Changes in circulating microRNAs levels with exercise modality
Banzet, S., Chennaoui, M., Girard, O., Racinais, S., Drogou, C., Chalabi, H., & Koulmann, N. (2013) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00075.2013 · Cited by 129
Profiling of Circulating MicroRNAs after a Bout of Acute Resistance Exercise in Humans
Sawada, S., Kon, M., Wada, S., Ushida, T., Suzuki, K., & Akimoto, T. (2013) · PLoS ONE · doi:10.1371/journal.pone.0070823 · Cited by 123 · cited 1×
“For example, it has been demonstrated that serum levels of the muscle-enriched miR-486 decrease after both acute and chronic aerobic exercise [9], while a resistance exercise bout of five sets of 10 repetitions at 70% of maximum strength, with 1 min rest between sets, resulted in an increase in miR-149-3p and a decrea…”
BackgroundHumoral Nature of the Hypoglycemic Factor of Muscular Work
Goldstein, M. S. (1961) · Diabetes · doi:10.2337/diab.10.3.232 · Cited by 105 · cited 1×
“Indeed, researchers have been searching for contraction-induced factors for more than fifty years [1].”
BackgroundSport and Exercise Physiology Testing Guidelines: Volume I - Sport Testing
Olivier, S. (2006) · Routledge eBooks · doi:10.4324/9780203966846 · Cited by 90
Guescini, M., Leo, G., Genedani, S., Carone, C., Pederzoli, F., Ciruela, F., Guidolin, D., Stocchi, V., Mantuano, M., Borroto‐Escuela, D. O., et al. (2012) · Experimental Cell Research · doi:10.1016/j.yexcr.2012.01.005 · Cited by 80 · cited 1×
“Indeed, a growing body of evidence demonstrates that EVs can act as intercellular communication devices acting as safe vesicular carriers of molecules such as receptors, transmembrane proteins, kinases, mRNA, miRNA, long noncoding RNA, DNA and lipids [54–56].”
BackgroundPredicting Fat Percent by Skinfolds in Racial Groups
Davidson, L. E., Wang, J., Thornton, J. C., Kaleem, Z. U., Silva-Palacios, F., Pierson, R. N., Heymsfield, S. B., & Gallagher, D. (2010) · Medicine & Science in Sports & Exercise · doi:10.1249/mss.0b013e3181ef3f07 · Cited by 61
Quantitative analysis of the secretion of the MCP family of chemokines by muscle cells
Henningsen, J., Pedersen, B. K., & Kratchmarova, I. (2010) · Molecular BioSystems · doi:10.1039/c0mb00209g · Cited by 51 · cited 1×
“other cytokines and several hundred proteins [3,4].”
Fas Signalling Promotes Intercellular Communication in T Cells
Luchetti, F., Canonico, B., Arcangeletti, M., Guescini, M., Cesarini, E., Stocchi, V., Esposti, M. D., & Papa, S. (2012) · PLoS ONE · doi:10.1371/journal.pone.0035766 · Cited by 49 · cited 2×
“EVs separated by ultracentrifugation were investigated for their expression of CD81, a well-established exosomal surface marker [36], and for the muscle marker SGCA.”
BackgroundMethodWinter..., M. E. (2016) · Cited by 23
Winter, E. M. (2007) · Cited by 1
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Extracellular Vesicles Provide a Means for Tissue Crosstalk during Exercise
Whitham, M., Parker, B. L., Friedrichsen, M. H., Hingst, J. R., Hjorth, M., Hughes, W. E., Egan, C. L., Cron, L., Watt, K. I., Kuchel, R. P., et al. (2018) · Cell Metabolism · doi:10.1016/j.cmet.2017.12.001 · Cited by 700
Brahmer, A., Neuberger, E. W. I., Esch-Heisser, L., Haller, N., Jørgensen, M. M., Bæk, R., Möbius, W., Simon, P. D., & Krämer‐Albers, E. (2019) · Journal of Extracellular Vesicles · doi:10.1080/20013078.2019.1615820 · Cited by 251
“exercise-related cell types such as muscle cells [alphasarcoglycan, [39], Figure 4(a)] or hepatocytes (asialoglycoprotein receptor, not shown) could not be detected.”
BackgroundFulzele, S., Mendhe, B., Khayrullin, A., Johnson, M. H., Kaiser, H., Liu, Y., Isales, C. M., & Hamrick, M. W. (2019) · Aging · doi:10.18632/aging.101874 · Cited by 220
Bei, Y., Xu, T., Lv, D., Yu, P., Xu, J., Che, L., Das, A., Tigges, J., Toxavidis, V., Ghiran, I. C., et al. (2017) · Basic Research in Cardiology · doi:10.1007/s00395-017-0628-z · Cited by 201
“In this regard, exercise-induced EVs derived from skeletal muscle deserve further investigation [1, 21, 40].”
Extracellular Vesicles and Exosomes: Insights From Exercise Science
Nederveen, J. P., Warnier, G., Carlo, A. D., Nilsson, M. I., & Tarnopolsky, M. A. (2021) · Frontiers in Physiology · doi:10.3389/fphys.2020.604274 · Cited by 200
“…of anaerobic threshold (Sossdorf et al., 2010, 2011), ∼70% of VO2max (Lansford et al., 2016) and a single bout of high intensity interval exercise (Guiraud et al., 2013) all appear to increase PMVs. Work by Wilhelm et al. (2016) examined PMVs during a 1 h bout of heavy intensity exercise (∼65%…”
Background
Metadata
- Authors
- Guescini, M., Canonico, B., Lucertini, F., Maggio, S., Annibalini, G., Barbieri, E., Luchetti, F., Papa, S., & Stocchi, V.
- Affiliations
- University of Urbino (IT)
- Corresponding author
- Guescini, Michele
- ORCID
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2015-05-08
- Volume
- 10
- Issue
- 5
- Pages
- e0125094
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC4425492
- OpenAlex
- W219060072
- Cited by
- 228
- References
- 62
- Citations by year
- 2026: 19 · 2025: 31 · 2024: 26 · 2023: 27 · 2022: 33 · 2021: 24 · 2020: 22 · 2019: 18 · 2018: 11 · 2017: 11 · 2016: 5 · 2015: 1
- FWCI
- 4.05
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Figshare (submittedVersion, cc-by)
- CINECA IRIS Institutional Research information system (University of Urbino) (submittedVersion, cc-by)
- Funders
- Regione Marche
- Università degli Studi di Urbino Carlo Bo
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Health Sciences › Medicine › Physiology (medical) › Exercise and Physiological Responses
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Keywords
- extracellular vesicles, alpha-sarcoglycan, miR-206, microRNA secretion, myokines, skeletal muscle, physical activity, cardiorespiratory fitness, miR-133b, miR-181a-5p, TSG101, CD81, plasma vesicles, muscle remodeling, muscle homeostasis
- MeSH terms
- Extracellular Vesicles, Tsg101 Protein, Adult, Cell Communication, Centrifugation, Density Gradient, DNA-Binding Proteins, Gene Expression Regulation, Humans, Male, Transcription Factors, Signal Transduction, Exercise, Muscle, Skeletal, MicroRNAs, Sarcoglycans, Exosomes, Endosomal Sorting Complexes Required for Transport, Sedentary Behavior, Tetraspanin 28
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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