Dynamic regulation of circulating microRNA during acute exhaustive exercise and sustained aerobic exercise training
Open accessAbstract
Non‐technical summary MicroRNA (miRNA) molecules are essential intracellular mediators of numerous biological processes including angiogenesis, inflammation, and mitochondrial metabolism. Recently, it has been shown that miRNAs are secreted into the bloodstream and that circulating miRNAs (c‐miRNAs) may serve important endocrine functions. This study examined plasma profiles of specific c‐miRNAs in healthy competitive athletes at rest and during exhaustive exercise testing, before and after a 90 day period of exercise training. In this setting, we observed four distinct patterns of c‐miRNA response to exercise: (1) c‐miRNAs up‐regulated by acute exhaustive exercise before and after sustained exercise training, (2) c‐miRNAs responsive to acute exhaustive exercise before but not after sustained exercise training, (3) c‐miRNAs responsive only to sustained exercise training, and (4) non‐responsive c‐miRNAs. These findings set the stage for further work aimed at defining the role of c‐miRNAs as fitness biomarkers and physiological mediators of exercise‐induced cardiovascular adaptation.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Exerkines include a broad range of signalling moieties such as cytokines, nucleic acids, lipids and metabolites, often carried by cell-specific extracellular vesicles.
“Exerkines are increasingly recognized to include a broad range of signalling moieties, including cytokines, nucleic acids (microRNA17, mRNA and mitochondrial DNA), lipids and metabolites, which are frequently driven by cell-specific extracellular vesicle secretion18.”
From Exerkines in health, resilience and disease (2022) · cites this paper as [17]
Acute exercise increases plasma levels of various microRNAs after exercise, and chronic exercise increases various microRNAs in the resting state.
“acute exercise increases plasma levels of various microRNAs after exercise, and chronic exercise increases various microRNAs in the resting state17”
From Exerkines in health, resilience and disease (2022) · cites this paper as [17]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Valadi, H., Ekström, K., Bossios, A., Sjöstrand, M., Lee, J. J., & Lötvall, J. O. (2007) · Nature Cell Biology · doi:10.1038/ncb1596 · Cited by 12,834 · see citing works
Lewis, B. P., Burge, C. B., & Bartel, D. P. (2005) · Cell · doi:10.1016/j.cell.2004.12.035 · Cited by 11,855 · see citing works
The functions of animal microRNAs
Ambros, V. (2004) · Nature · doi:10.1038/nature02871 · Cited by 10,728 · see citing works
Circulating microRNAs as stable blood-based markers for cancer detection
Mitchell, P. S., Parkin, R. K., Kroh, E. M., Fritz, B. R., Wyman, S. K., Pogosova-Agadjanyan, E. L., Peterson, A., Noteboom, J., O'Briant, K. C., Allen, A., et al. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0804549105 · Cited by 7,969 · see citing works
Taganov, K. D., Boldin, M. P., Chang, K.-J., & Baltimore, D. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0605298103 · Cited by 4,317 · see citing works
A new method for detecting anaerobic threshold by gas exchange
Beaver, W. L., Wasserman, K., & Whipp, B. J. (1986) · Journal of Applied Physiology · doi:10.1152/jappl.1986.60.6.2020 · Cited by 4,103 · see citing works
The role of microRNA-1 and microRNA-133 in skeletal muscle proliferation and differentiation
Chen, J., Mandel, E. M., Thomson, J. M., Wu, Q., Callis, T. E., Hammond, S. M., Conlon, F. L., & Wang, D.-Z. (2005) · Nature Genetics · doi:10.1038/ng1725 · Cited by 2,763 · see citing works
MicroRNA-21 contributes to myocardial disease by stimulating MAP kinase signalling in fibroblasts
Thum, T., Gross, C., Fiedler, J., Fischer, T., Kissler, S., Bussen, M., Galuppo, P., Just, S., Rottbauer, W., Frantz, S., et al. (2008) · Nature · doi:10.1038/nature07511 · Cited by 2,349 · see citing works
Secretory Mechanisms and Intercellular Transfer of MicroRNAs in Living Cells
Kosaka, N., Iguchi, H., Yoshioka, Y., Takeshita, F., Matsuki, Y., & Ochiya, T. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.107821 · Cited by 1,950 · see citing works
MicroRNA-133 controls cardiac hypertrophy
Carè, A., Catalucci, D., Felicetti, F., Bonci, D., Addario, A., Gallo, P., Bang, M., Segnalini, P., Gu, Y., Dalton, N. D., et al. (2007) · Nature Medicine · doi:10.1038/nm1582 · Cited by 1,775 · see citing works
Functional delivery of viral miRNAs via exosomes
Pegtel, D. M., Cosmopoulos, K., Thorley‐Lawson, D. A., van Eijndhoven, M. A. J., Hopmans, E. S., Lindenberg, J. L., de Gruijl, T. D., Wurdinger, T. D., & Middeldorp, J. M. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0914843107 · Cited by 1,648 · see citing works
Circulating microRNA in body fluid: a new potential biomarker for cancer diagnosis and prognosis
Kosaka, N., Iguchi, H., & Ochiya, T. (2010) · Cancer Science · doi:10.1111/j.1349-7006.2010.01650.x · Cited by 1,335 · see citing works
SMAD proteins control DROSHA-mediated microRNA maturation
Davis‐Dusenbery, B. N., Hilyard, A. C., Lagna, G., & Hata, A. (2008) · Nature · doi:10.1038/nature07086 · Cited by 1,243 · see citing works
Circulating MicroRNAs in Patients With Coronary Artery Disease
Fichtlscherer, S., Rosa, S. D., Fox, H., Schwietz, T., Fischer, A., Liebetrau, C., Weber, M. A., Hamm, C. W., Roexe, T., Müller-Ardogan, M., et al. (2010) · Circulation Research · doi:10.1161/circresaha.109.215566 · Cited by 1,222 · see citing works
Secreted Monocytic miR-150 Enhances Targeted Endothelial Cell Migration
Zhang, Y., Liu, D., Chen, X., Li, J., Li, L., Bian, Z., Sun, F., Lu, J., Yin, Y., Cai, X. F., et al. (2010) · Molecular Cell · doi:10.1016/j.molcel.2010.06.010 · Cited by 1,190 · see citing works
Augmentation of tumor angiogenesis by a Myc-activated microRNA cluster
Dews, M., Homayouni, A., Yu, D., Murphy, D. A., Sevignani, C., Wentzel, E. A., Furth, E. E., Lee, W. M., Enders, G. H., Mendell, J. T., et al. (2006) · Nature Genetics · doi:10.1038/ng1855 · Cited by 1,049 · see citing works
miR‐21: a small multi‐faceted RNA
Krichevsky, A. M., & Gabriely, G. (2009) · Journal of Cellular and Molecular Medicine · doi:10.1111/j.1582-4934.2008.00556.x · Cited by 987 · see citing works
Fasanaro, P., D'Alessandra, Y., Di Stefano, V., Melchionna, R., Romani, S., Pompilio, G., Capogrossi, M. C., & Martelli, F. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m800731200 · Cited by 912 · see citing works
Role of microRNAs in vascular diseases, inflammation, and angiogenesis
Urbich, C., Kuehbacher, A., & Dimmeler, S. (2008) · Cardiovascular Research · doi:10.1093/cvr/cvn156 · Cited by 874 · see citing works
Iliopoulos, D., Jaeger, S. A., Hirsch, H. A., Bulyk, M. L., & Struhl, K. (2010) · Molecular Cell · doi:10.1016/j.molcel.2010.07.023 · Cited by 830 · see citing works
Role of Dicer and Drosha for Endothelial MicroRNA Expression and Angiogenesis
Kuehbacher, A., Urbich, C., Zeiher, A. M., & Dimmeler, S. (2007) · Circulation Research · doi:10.1161/circresaha.107.153916 · Cited by 782 · see citing works
MicroRNAs modulate the angiogenic properties of HUVECs
Poliseno, L., Tuccoli, A., Mariani, L., Evangelista, M., Citti, L., Woods, K., Mercatanti, A., Hammond, S. M., & Rainaldi, G. (2006) · Blood · doi:10.1182/blood-2006-01-012369 · Cited by 754 · see citing works
Chan, S. Y., Zhang, Y.-Y., Hemann, C., Mahoney, C. E., Zweier, J. L., & Loscalzo, J. (2009) · Cell Metabolism · doi:10.1016/j.cmet.2009.08.015 · Cited by 702 · see citing works
Self-citationDicer Dependent MicroRNAs Regulate Gene Expression and Functions in Human Endothelial Cells
Suárez, Y., Fernandez-Hernando, C. F., Pober, J. S., & Sessa, W. C. (2007) · Circulation Research · doi:10.1161/01.res.0000265065.26744.17 · Cited by 698 · see citing works
Analysis of Circulating MicroRNA: Preanalytical and Analytical Challenges
McDonald, J. S., Milosevic, D., Reddi, H. V., Grebe, S. K., & Algeciras‐Schimnich, A. (2011) · Clinical Chemistry · doi:10.1373/clinchem.2010.157198 · Cited by 684 · see citing works
Plasma MicroRNAs as Sensitive and Specific Biomarkers of Tissue Injury
Laterza, O., Lim, L. P., Garrett-Engele, P. W., Vlasáková, K., Muniappa, N., Tanaka, W. K., Johnson, J. M., Sina, J. F., Fare, T. L., Sistare, F. D., et al. (2009) · Clinical Chemistry · doi:10.1373/clinchem.2009.131797 · Cited by 618 · see citing works
MicroRNAs: powerful new regulators of heart disease and provocative therapeutic targets
van Rooij, E., & Olson, E. N. (2007) · Journal of Clinical Investigation · doi:10.1172/jci33099 · Cited by 552 · see citing works
Davidsen, P. K., Gallagher, I. J., Hartman, J. W., Tarnopolsky, M. A., Dela, F., Helge, J. W., Timmons, J. A., & Phillips, S. M. (2011) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00901.2010 · Cited by 385 · see citing works
Rethinking the Warburg Effect with Myc Micromanaging Glutamine Metabolism
Dang, C. V. (2010) · Cancer Research · doi:10.1158/0008-5472.can-09-3556 · Cited by 379 · see citing works
MicroRNA-210: A unique and pleiotropic hypoxamir
Chan, S. Y., & Loscalzo, J. (2010) · Cell Cycle · doi:10.4161/cc.9.6.11006 · Cited by 357 · see citing works
Self-citationMicroRNA control of muscle development and disease
Williams, A. H., Liu, N., van Rooij, E., & Olson, E. N. (2009) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2009.01.029 · Cited by 347 · see citing works
Baggish, A. L., Wang, F., Weiner, R. B., Elinoff, J. M., Tournoux, F., Boland, A., Picard, M. H., Hutter, A. M., & Wood, M. J. (2007) · Journal of Applied Physiology · doi:10.1152/japplphysiol.01170.2007 · Cited by 345 · see citing works
Self-citationMuscle specific microRNAs are regulated by endurance exercise in human skeletal muscle
Nielsen, S. J., Schéele, C., Yfanti, C., Akerstrom, T. C. A., Nielsen, A. R., Pedersen, B. K., & Laye, M. J. (2010) · The Journal of Physiology · doi:10.1113/jphysiol.2010.189860 · Cited by 340 · see citing works
Cheng, Y., Zhu, P., Yang, J., Liu, X., Dong, S., Wang, X., Chun, B., Zhuang, J., & Zhang, C. (2010) · Cardiovascular Research · doi:10.1093/cvr/cvq082 · Cited by 331 · see citing works
Circulating MicroRNAs as Biomarkers and Potential Paracrine Mediators of Cardiovascular Disease
Gupta, S. K., Bang, C., & Thum, T. (2010) · Circulation Cardiovascular Genetics · doi:10.1161/circgenetics.110.958363 · Cited by 292 · see citing works
Exercise training-induced hypervolemia: role of plasma albumin, renin, and vasopressin
Convertino, V. A., Brock, P. J. M., KEIL, L. C., Bernauer, E. M., & GREENLEAF, J. E. (1980) · Journal of Applied Physiology · doi:10.1152/jappl.1980.48.4.665 · Cited by 272 · see citing works
Plasma volume, osmolality, vasopressin, and renin activity during graded exercise in man
Convertino, V. A., KEIL, L. C., Bernauer, E. M., & GREENLEAF, J. E. (1981) · Journal of Applied Physiology · doi:10.1152/jappl.1981.50.1.123 · Cited by 259 · see citing works
MiRNAs as biomarkers and therapeutic targets in cancer
Heneghan, H., Miller, N., & Kerin, M. J. (2010) · Current Opinion in Pharmacology · doi:10.1016/j.coph.2010.05.010 · Cited by 252 · see citing works
Safdar, A., Abadi, A., Akhtar, M., Hettinga, B. P., & Tarnopolsky, M. A. (2009) · PLoS ONE · doi:10.1371/journal.pone.0005610 · Cited by 238 · see citing works
The microRNA miR-696 regulates PGC-1α in mouse skeletal muscle in response to physical activity
Aoi, W., Naito, Y., Mizushima, K., Takanami, Y., Kawai, Y., Ichikawa, H., & Yoshikawa, T. (2010) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00448.2009 · Cited by 206 · see citing works
Time course of molecular responses of human skeletal muscle to acute bouts of resistance exercise
Bickel, C. S., Slade, J. M., Mahoney, E., Haddad, F., Dudley, G. A., & Adams, G. R. (2005) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00895.2004 · Cited by 200 · see citing works
Human Muscle Gene Expression following Resistance Exercise and Blood Flow Restriction
Drummond, M. J., Fujita, S., Abe, T., Dreyer, H. C., Volpi, E., & Rasmussen, B. B. (2008) · Medicine & Science in Sports & Exercise · doi:10.1249/mss.0b013e318160ff84 · Cited by 187 · see citing works
The physiological regulation of toll‐like receptor expression and function in humans
Lancaster, G. I., Khan, Q., Drysdale, P., Wallace, F., Jeukendrup, A. E., Drayson, M. T., & Gleeson, M. (2005) · The Journal of Physiology · doi:10.1113/jphysiol.2004.081224 · Cited by 176 · see citing works
MicroRNAs of the immune system
Davidson-Moncada, J. K., Papavasiliou, F. N., & Tam, W. (2010) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.2009.05121.x · Cited by 171 · see citing works
Evidence for microRNA involvement in exercise-associated neutrophil gene expression changes
Radom‐Aizik, S., Zaldivar, F. P., Oliver, S. R., Galassetti, P. R., & Cooper, D. M. (2010) · Journal of Applied Physiology · doi:10.1152/japplphysiol.01291.2009 · Cited by 151 · see citing works
Regional changes in capillary supply in skeletal muscle of high-intensity endurance-trained rats
Gute, D. C., Fraga, C., Laughlin, M. H., & Amann, J. F. (1996) · Journal of Applied Physiology · doi:10.1152/jappl.1996.81.2.619 · Cited by 121 · see citing works
Liu, G., Keeler, B. E., Zhukareva, V. A., et al. (2010) · Experimental Neurology · doi:10.1016/j.expneurol.2010.08.032 · Cited by 109 · see citing works
MicroRNA miR-328 Regulates Zonation Morphogenesis by Targeting CD44 Expression
Wang, C.-H., Lee, D. Y., Deng, Z., Jeyapalan, Z., Lee, S.-C., Kahai, S., Lu, W.-Y., Zhang, Y., & Yang, B. B. (2008) · PLoS ONE · doi:10.1371/journal.pone.0002420 · Cited by 87 · see citing works
MicroRNAs in Vascular Biology and Vascular Disease
Zhang, C. (2010) · Journal of Cardiovascular Translational Research · doi:10.1007/s12265-010-9164-z · Cited by 74 · see citing works
Kiessling, K., Pilström, L., Karlsson, J., & Piehl, K. (1973) · Clinical Science · doi:10.1042/cs0440547 · Cited by 74 · see citing works
Eccentric Exercise and Muscle Damage
Clarkson, P. M. (1997) · International Journal of Sports Medicine · doi:10.1055/s-2007-972741 · Cited by 71 · see citing works
Time course of molecular responses of human skeletal muscle to acute bouts of resistance exercise
Bickel, C. S., Slade, J. M., Mahoney, E. T., Haddad, F., Dudley, G. A., & Adams, G. (2005) · Scandinavian Journal of Medicine and Science in Sports · doi:10.1111/j.1600-0838.2005.453_2.x · Cited by 50 · see citing works
Effects of Preexercise Feeding on Markers of Satellite Cell Activation
Roberts, M. D., Dalbo, V. J., Hassell, S. E., BROWN, R., & Kerksick, C. M. (2010) · Medicine & Science in Sports & Exercise · doi:10.1249/mss.0b013e3181da8a29 · Cited by 32 · see citing works
Stuart, C. A., Wen, G., Williamson, M. E., Jiang, J., Gilkison, C. R., Blackwell, S. J., Nagamani, M., & Ferrando, A. A. (2001) · Metabolism · doi:10.1053/meta.2001.24202 · Cited by 31 · see citing works
Wessner, B., Gryadunov-Masutti, L., Tschan, H., Bachl, N., & Roth, E. (2026) · PubMed · doi:10.22029/eir.2010.1649 · Cited by 17 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Exerkines in health, resilience and disease
Chow, L. S., Gerszten, R. E., Taylor, J. M., Pedersen, B. K., van Praag, H., Trappe, S., Febbraio, M. A., Galis, Z. S., Gao, Y., Haus, J. M., et al. (2022) · Nature Reviews Endocrinology · doi:10.1038/s41574-022-00641-2 · Cited by 927 · see citing works
Skeletal Muscle as an Endocrine Organ: The Role of Myokines in Exercise Adaptations
Hoffmann, C., & Weigert, C. (2017) · Cold Spring Harbor Perspectives in Medicine · doi:10.1101/cshperspect.a029793 · Cited by 393 · see citing works
Muscle releases alpha-sarcoglycan positive extracellular vesicles carrying miRNAs in the bloodstream
Guescini, M., Canonico, B., Lucertini, F., Maggio, S., Annibalini, G., Barbieri, E., Luchetti, F., Papa, S., & Stocchi, V. (2015) · PLoS ONE · doi:10.1371/journal.pone.0125094 · Cited by 228 · see citing works
“[7], using other exercise protocols, researchers have shown the modulation of specific myomiRs in blood.”
BackgroundMethodInfluentialGuo, W., Bachman, E., Li, M., Roy, C. N., Blusztajn, J., Wong, S., Chan, S. Y., Serra, C., Jasuja, R., Travison, T. G., et al. (2013) · Aging Cell · doi:10.1111/acel.12052 · Cited by 192 · see citing works
Self-citationExercise and non-pharmacological treatment of POTS
Fu, Q., & Levine, B. D. (2018) · Autonomic Neuroscience · doi:10.1016/j.autneu.2018.07.001 · Cited by 174 · see citing works
Physical exercise and epigenetic modifications in skeletal muscle
Widmann, M., Nieß, A. M., & Munz, B. (2019) · Sports Medicine · doi:10.1007/s40279-019-01070-4 · Cited by 165 · see citing works
Diagnosis, pathophysiology, and management of exercise-induced arrhythmias
Guasch, E., & Mont, L. (2017) · Nature Reviews Cardiology · doi:10.1038/nrcardio.2016.173 · Cited by 134 · see citing works
De Gasperi, R., Hamidi, S., Harlow, L. M., Ksiezak-Reding, H., Bauman, W. A., & Cardozo, C. P. (2017) · Scientific Reports · doi:10.1038/s41598-017-13105-9 · Cited by 75 · see citing works
Atrial Fibrillation in Elite Athletes: A Comprehensive Review of the Literature
Kourek, C., Briasoulis, A., Tsougos, E., & Paraskevaidis, I. (2024) · Journal of Cardiovascular Development and Disease · doi:10.3390/jcdd11100315 · Cited by 7 · see citing works
“…recurrent AF in the general population [66–68], including galectin-3 [69], the suppression of tumorigenicity 2 (ST2) [70], and microRNA-21 (miR-21) [71], as well as tissue inhibitors of metalloproteinase 1 (TIMP-1), C-terminal telopeptide of type I collagen (CITP), and procollagen type I…”
Background
- Authors
- Baggish, A. L., Hale, A., Weiner, R. B., Lewis, G. D., Systrom, D., Wang, F., Wang, T. J., & Chan, S. Y.
- Affiliations
- Harvard University (US)
- Brigham and Women's Hospital (US)
- ORCID
- Venue
- The Journal of Physiology
- Publisher
- Wiley
- Published
- 2011-08
- Volume
- 589
- Issue
- 16
- Pages
- 3983-3994
- ISSN
- 0022-3751 (ISSN-L)
- 1469-7793
- PMC
- PMC3179997
- OpenAlex
- W2157038349
- Cited by
- 431
- References
- 55
- Citations by year
- 2026: 13 · 2025: 27 · 2024: 25 · 2023: 17 · 2022: 31 · 2021: 36 · 2020: 32 · 2019: 42 · 2018: 41 · 2017: 34 · 2016: 33 · 2015: 36 · 2014: 29 · 2013: 23 · 2012: 11
- FWCI
- 20.76
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- The Journal of Physiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Heart, Lung, and Blood Institute (K08HL096834, K23HL091106)
- Topics
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Keywords
- circulating microRNAs, acute exhaustive exercise, aerobic exercise training, rowing, cycling exercise, miR-146a, miR-20a, miR-21, miR-222, miR-210, miR-133a, miR-221, miR-328, exercise biomarkers, VO2max, cardiovascular adaptation, inflammation, angiogenesis, muscle contractility
- MeSH terms
- Humans, Longitudinal Studies, Male, Physical Endurance, Prospective Studies, Time Factors, Cohort Studies, Exercise, MicroRNAs, Young Adult, Athletes
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
