Use of Lactobacillus crispatus to produce a probiotic cheese as potential gender food for preventing gynaecological infections
Open accessAbstract
This research is aimed to evaluate the suitability of Squacquerone cheese to support the viability of Lactobacillus crispatus BC4, a vaginal strain endowed with a strong antimicrobial activity against urogenital pathogens and foodborne microorganisms, in order to recommend a gender food for woman wellbeing. The viability of L. crispatus BC4, used as adjunct culture, was evaluated during the refrigerated storage of Squacquerone cheese, as well as when the cheese was subjected to simulated stomach-duodenum passage tested by the patented Simulator of the Human Intestinal Microbial Ecosystem (SHIME). Moreover, the effects of L. crispatus BC4 addition were evaluated on product hydrolytic patterns, in terms of proteolysis, lipolysis and volatile molecule profiles. The data showed that L. crispatus BC4 maintained high viability, also in presence of physiological stress conditions, until the end of the refrigerated storage. Moreover, the inclusion of L. crispatus BC4 gave rise to cheese product with higher score of overall acceptability when compared to control cheese. In addition, the survival of L. crispatus BC4, carried in test cheese, in gastro intestinal conditions was confirmed by SHIME. The results showed that the vaginal Lactobacillus strain was more affected by the low pH of the stomach, simulated by the SHIME reactor, rather than to bile salts and pancreatic juices. Although only in vivo trials will be able to confirm the functionality of the cheese in the vaginal environment, these data represent a first step towards the employment of the Squacquerone cheese as probiotic food able to promote the woman's health by preventing gynaecological infections.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Some cheeses are inoculated with live cultures of L. acidophilus and bifidobacterium, and blue cheese and aged cheeses are good sources of probiotics.
“Some cheeses are inoculated with live cultures of L. acidophilus and bifidobacterium. Other cheeses that are good sources of probiotics are blue cheese and aged cheeses [188,189].”
From The Potential Impact of Probiotics on Human Health: An Update on Their Health-Promoting Properties (2024) · cites this paper as [188]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Sexually transmitted diseases treatment guidelines (2015)
Workowski, K., & BOLAN, G. A. (2015) · Reproductive Endocrinology · doi:10.18370/2309-4117.2015.24.51-56 · Cited by 2,689 · see citing works
Sexually Transmitted Diseases Treatment Guidelines, 2010
Workowski, K., & BOLAN, G. A. (2011) · Annals of Emergency Medicine · doi:10.1016/j.annemergmed.2011.04.006 · Cited by 2,074 · see citing works
Functional food. Product development, marketing and consumer acceptance—a review
Siró, I., Kápolna, E., Kápolna, B., & Lugasi, A. (2008) · Appetite · doi:10.1016/j.appet.2008.05.060 · Cited by 1,758 · see citing works
Interactions between the microbiota and pathogenic bacteria in the gut
Bäumler, A. J., & Sperandio, V. (2016) · Nature · doi:10.1038/nature18849 · Cited by 1,420 · see citing works
Host Biology in Light of the Microbiome: Ten Principles of Holobionts and Hologenomes
Bordenstein, S. R., & Theis, K. R. (2015) · PLOS Biology · doi:10.1371/journal.pbio.1002226 · Cited by 1,240 · see citing works
A Symbiotic View of Life: We Have Never Been Individuals
Gilbert, S. F., Sapp, J., & Tauber, A. I. (2012) · The Quarterly Review of Biology · doi:10.1086/668166 · Cited by 1,208 · see citing works
Probiotic functional foods: Survival of probiotics during processing and storage
Tripathi, M. K., & Giri, S. K. (2014) · Journal of Functional Foods · doi:10.1016/j.jff.2014.04.030 · Cited by 1,181 · see citing works
(1982) · Milk science international/Milchwissenschaft · Cited by 702 · see citing works
Molly, K., Vande Woestyne, M., & Verstraete, W. (1993) · Applied Microbiology and Biotechnology · doi:10.1007/bf00228615 · Cited by 672 · see citing works
Development of the gut microbiota in infancy and its impact on health in later life
Tanaka, M., & Nakayama, J. (2017) · Allergology International · doi:10.1016/j.alit.2017.07.010 · Cited by 639 · see citing works
The role of lactic acid production by probiotic Lactobacillus species in vaginal health
Tachedjian, G., Aldunate, M., Bradshaw, C. S., & Cone, R. A. (2017) · Research in Microbiology · doi:10.1016/j.resmic.2017.04.001 · Cited by 557 · see citing works
Probiotics, their health benefits and applications for developing healthier foods: a review
Nagpal, R., Kumar, A., Kumar, M., Behare, P. V., Jain, S., & Yadav, H. (2012) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2012.02593.x · Cited by 496 · see citing works
Oral probiotics can resolve urogenital infections
Reid, G., Bruce, A. W., Fraser, N., Heinemann, C., Owen, J., & Henning, B. (2001) · FEMS Immunology & Medical Microbiology · doi:10.1016/s0928-8244(00)00233-9 · Cited by 464 · see citing works
The role of lactobacilli and probiotics in maintaining vaginal health
Borges, S., Silva, J., & Teixeira, P. (2014) · Archives of Gynecology and Obstetrics · doi:10.1007/s00404-013-3064-9 · Cited by 397 · see citing works
Dahiya, D. K., Renuka, Puniya, M., Shandilya, U. K., Dhewa, T., Kumar, N., Kumar, S., Puniya, A. K., & Shukla, P. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.00563 · Cited by 357 · see citing works
Sexually Transmitted Diseases Treatment Guidelines, 2006
Workowski, K., & Berman, S. M. (2006) · PsycEXTRA Dataset · doi:10.1037/e528752006-001 · Cited by 264 · see citing works
da Silva, B. V., Barreira, J. C. M., & Oliveira, M. B. P. P. (2016) · Trends in Food Science & Technology · doi:10.1016/j.tifs.2015.12.007 · Cited by 248 · see citing works
Women and Their Microbes: The Unexpected Friendship
Younes, J. A., Lievens, E., Hummelen, R., van der Westen, R., Reid, G., & Petrova, M. (2017) · Trends in Microbiology · doi:10.1016/j.tim.2017.07.008 · Cited by 245 · see citing works
Use of Lactobacillus probiotics for bacterial genitourinary infections in women: A review
Barrons, R., & Tassone, D. (2008) · Clinical Therapeutics · doi:10.1016/j.clinthera.2008.03.013 · Cited by 240 · see citing works
Angelis, M. D., Siragusa, S., Berloco, M., Caputo, L., Settanni, L., Alfonsi, G., Amerio, M., Grandi, A., Ragni, A., & Gobbetti, M. (2006) · Research in Microbiology · doi:10.1016/j.resmic.2006.05.003 · Cited by 240 · see citing works
Isolation of vaginal lactobacilli and characterization of anti-candida activity
Parolin, C., Marangoni, A., Laghi, L., Foschi, C., Ñahui Palomino, R. A., Calonghi, N., Cevenini, R., & Vitali, B. (2015) · PLOS ONE · doi:10.1371/journal.pone.0131220 · Cited by 228 · see citing works
Self-citationGovender, M., Choonara, Y. E., Kumar, P., du Toit, L. C., van Vuuren, S. F., & Pillay, V. (2013) · AAPS PharmSciTech · doi:10.1208/s12249-013-0027-1 · Cited by 215 · see citing works
Hossain, M. I., Sadekuzzaman, M., & Ha, S. (2017) · Food Research International · doi:10.1016/j.foodres.2017.07.077 · Cited by 210 · see citing works
von Martels, J. Z. H., Sadabad, M. S., Bourgonje, A. R., Blokzijl, T., Dijkstra, G. D. H., Faber, K. N., & Harmsen, H. J. M. (2017) · Anaerobe · doi:10.1016/j.anaerobe.2017.01.001 · Cited by 184 · see citing works
Rajoka, M. S. R., Shi, J., Mehwish, H. M., Zhu, J., Li, Q., Shao, D., Huang, Q., & Yang, H. (2017) · Food Science and Human Wellness · doi:10.1016/j.fshw.2017.07.003 · Cited by 167 · see citing works
Lindsay, K. L., Kennelly, M. A., Culliton, M., Smith, T., Maguire, O. C., Shanahan, F., Brennan, L., & McAuliffe, F. M. (2014) · American Journal of Clinical Nutrition · doi:10.3945/ajcn.113.079723 · Cited by 160 · see citing works
Vujic, G., Jajac Knez, A., Despot Stefanovic, V., & Kuzmic Vrbanovic, V. (2013) · European Journal of Obstetrics & Gynecology and Reproductive Biology · doi:10.1016/j.ejogrb.2012.12.031 · Cited by 159 · see citing works
Nutrition and human health from a sex–gender perspective
Marino, M., Masella, R., Bulzomi, P., Campesi, I., Malorni, W., & Franconi, F. (2011) · Molecular Aspects of Medicine · doi:10.1016/j.mam.2011.02.001 · Cited by 152 · see citing works
Production of Crescenza Cheese by Incorporation of Bifidobacteria
Gobbetti, M., Corsetti, A., Smacchi, E., et al. (1998) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(98)75548-1 · Cited by 150 · see citing works
Changes in vaginal microbiota following antimicrobial and probiotic therapy
Macklaim, J. M., Clemente, J. C., Knight, R., Gloor, G. B., & Reid, G. (2015) · Microbial Ecology in Health & Disease · doi:10.3402/mehd.v26.27799 · Cited by 134 · see citing works
Nardini, P., Palomino, R. A. Ñ., Parolin, C., Laghi, L., Foschi, C., Cevenini, R., Vitali, B., & Marangoni, A. (2016) · Scientific Reports · doi:10.1038/srep29024 · Cited by 129 · see citing works
Self-citationGene editing and genetic engineering approaches for advanced probiotics: A review
Yadav, R., Kumar, V., Baweja, M., & Shukla, P. (2017) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2016.1274877 · Cited by 118 · see citing works
The Gut Microbiota and Human Health with an Emphasis on the Use of Microencapsulated Bacterial Cells
Prakash, S., Tomaro-Duchesneau, C., Saha, S., & Cantor, A. (2011) · BioMed Research International · doi:10.1155/2011/981214 · Cited by 115 · see citing works
Gut microbiota and probiotics in maternal and infant health
Sanz, Y. (2011) · American Journal of Clinical Nutrition · doi:10.3945/ajcn.110.001172 · Cited by 111 · see citing works
Burns, P., Patrignani, F., Serrazanetti, D. I., et al. (2008) · Journal of Dairy Science · doi:10.3168/jds.2007-0516 · Cited by 110 · see citing works
Self-citationRizzo, A., Losacco, A., & Carratelli, C. R. (2013) · Immunology Letters · doi:10.1016/j.imlet.2013.08.013 · Cited by 108 · see citing works
Vitali, B., Cruciani, F., Baldassarre, M. E., Capursi, T., Spisni, E., Valerii, M. C., Candela, M., Turroni, S., & Brigidi, P. (2012) · BMC Microbiology · doi:10.1186/1471-2180-12-236 · Cited by 107 · see citing works
Self-citationHeczko, P. B., Tomusiak, A., Adamski, P., Jakimiuk, A. J., Stefański, G., Mikołajczyk-Cichońska, A., Suda-Szczurek, M., & Strus, M. (2015) · BMC Women s Health · doi:10.1186/s12905-015-0246-6 · Cited by 106 · see citing works
Mouthfeel and flavour of fermented whey with added hydrocolloids
Gallardo-Escamilla, F., Kelly, A. L., & Delahunty, C. M. (2006) · International Dairy Journal · doi:10.1016/j.idairyj.2006.04.009 · Cited by 100 · see citing works
Rizzo, A., Fiorentino, M., Buommino, E., Donnarumma, G., Losacco, A., & Bevilacqua, N. (2015) · International Journal of Medical Microbiology · doi:10.1016/j.ijmm.2015.07.005 · Cited by 93 · see citing works
Strus, M., Chmielarczyk, A., Kochan, P., Adamski, P., Chełmicki, Z., Chełmicki, A., Pałucha, A., & Heczko, P. B. (2012) · European Journal of Obstetrics & Gynecology and Reproductive Biology · doi:10.1016/j.ejogrb.2012.05.001 · Cited by 91 · see citing works
Foschi, C., Salvo, M., Cevenini, R., Parolin, C., Vitali, B., & Marangoni, A. (2017) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2017.00502 · Cited by 86 · see citing works
Self-citationBohbot, J.-M., Daraï, É., Bretelle, F., Brami, G., Daniel, C., & Cardot, J.-M. (2017) · Journal of Gynecology Obstetrics and Human Reproduction · doi:10.1016/j.jogoh.2017.11.005 · Cited by 85 · see citing works
Vaginal Lactobacillus Inhibits HIV-1 Replication in Human Tissues Ex Vivo
Palomino, R. A. Ñ., Zicari, S., Vanpouille, C., Vitali, B., & Margolis, L. B. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.00906 · Cited by 77 · see citing works
Self-citationBecker, A. (2015) · Frontiers in Microbiology · doi:10.3389/fmicb.2015.00687 · Cited by 74 · see citing works
An overview of advanced technologies for selection of probiotics and their expediency: A review
Yadav, R., & Shukla, P. (2015) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2015.1108957 · Cited by 73 · see citing works
Local Treatment of Vulvovaginal Candidosis
das Neves, J., Pinto, E., Teixeira, B., Dias, G., Rocha, P., Cunha, T., Santos, B., Amaral, M. H., & Bahia, M. F. (2008) · Drugs · doi:10.2165/00003495-200868130-00002 · Cited by 69 · see citing works
Diagnostic and therapeutic advancements for aerobic vaginitis
Han, C., Wu, W., Fan, A., et al. (2014) · Archives of Gynecology and Obstetrics · doi:10.1007/s00404-014-3525-9 · Cited by 69 · see citing works
Farhangi, M. A., Javid, A. Z., Sarmadi, B., Karimi, P., & Dehghan, P. (2018) · Clinical Nutrition · doi:10.1016/j.clnu.2017.06.005 · Cited by 68 · see citing works
Patrignani, F., Iucci, L., Lanciotti, R., et al. (2007) · Journal of Dairy Science · doi:10.3168/jds.2007-0373 · Cited by 61 · see citing works
Self-citationDols, J. A. M., Smit, P. W., Kort, R., Reid, G., Schuren, F. H. J., Tempelman, H. A., Bontekoe, T. R., Korporaal, H., & Boon, M. E. (2011) · American Journal of Obstetrics and Gynecology · doi:10.1016/j.ajog.2010.11.012 · Cited by 60 · see citing works
Siroli, L., Patrignani, F., Serrazanetti, D. I., Parolin, C., Palomino, R. A. Ñ., Vitali, B., & Lanciotti, R. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.00166 · Cited by 59 · see citing works
Self-citationReview of the role of probiotics in gastrointestinal diseases in adults
Domingo, J. J. S. (2017) · Gastroenterología y Hepatología (English Edition) · doi:10.1016/j.gastre.2016.12.001 · Cited by 59 · see citing works
Vannini, L., Patrignani, F., Iucci, L., Ndagijimana, M., Vallicelli, M., Lanciotti, R., & Guerzoni, M. E. (2008) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2008.09.018 · Cited by 58 · see citing works
Self-citationVinderola, G., CÉSPEDES, M., Mateolli, D., et al. (2011) · International Journal of Dairy Technology · doi:10.1111/j.1471-0307.2010.00659.x · Cited by 52 · see citing works
Tofalo, R., Schirone, M., Fasoli, G., et al. (2014) · Food Chemistry · doi:10.1016/j.foodchem.2014.11.088 · Cited by 50 · see citing works
Potential of high pressure homogenisation on probiotic Caciotta cheese quality and functionality
Burns, P., Patrignani, F., Tabanelli, G., Vinderola, G., Siroli, L., Reinheimer, J. A., Gardini, F., & Lanciotti, R. (2015) · Journal of Functional Foods · doi:10.1016/j.jff.2014.12.037 · Cited by 48 · see citing works
Self-citationHeart disease among post-menopausal women: Acceptability of functional foods as a preventive measure
Korzen-Bohr, S., & Jensen, K. O. (2006) · Appetite · doi:10.1016/j.appet.2005.11.003 · Cited by 44 · see citing works
Yadav, R., Singh, P. K., Puniya, A. K., & Shukla, P. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.02116 · Cited by 43 · see citing works
Revisión del papel de los probióticos en la patología gastrointestinal del adulto
Domingo, J. J. S. (2017) · Gastroenterología y Hepatología · doi:10.1016/j.gastrohep.2016.12.003 · Cited by 42 · see citing works
Clinical effects of probiotics in cystic fibrosis patients: A systematic review
Biervliet, S. V., Declercq, D., & Somerset, S. M. (2017) · Clinical Nutrition ESPEN · doi:10.1016/j.clnesp.2017.01.007 · Cited by 39 · see citing works
Kimoto‐Nira, H., Nagakura, Y., Kodama, C., SHIMIZU, T., Okuta, M., Sasaki, K., Koikawa, N., Sakuraba, K., Suzuki, C., & Suzuki, Y. (2014) · Journal of Dairy Science · doi:10.3168/jds.2014-7980 · Cited by 38 · see citing works
Vitali, B., Abruzzo, A., Parolin, C., Palomino, R. A. Ñ., Dalena, F., Bigucci, F., Cerchiara, T., & Luppi, B. (2015) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2015.10.035 · Cited by 32 · see citing works
Self-citationUrinary metabolomic fingerprinting after consumption of a probiotic strain in women with mastitis
Vázquez‐Fresno, R., Llorach, R., Marinić, J., Tulipani, S., Garcia‐Aloy, M., Espinosa‐Martos, I., Jiménez, E., Rodríguez, J. M., & Andrés‐Lacueva, C. (2014) · Pharmacological Research · doi:10.1016/j.phrs.2014.05.010 · Cited by 31 · see citing works
Patrignani, F., Serrazanetti, D. I., Mathara, J. M., Siroli, L., Gardini, F., Holzapfel, W. H., & Lanciotti, R. (2015) · International Journal of Dairy Technology · doi:10.1111/1471-0307.12251 · Cited by 29 · see citing works
Self-citationKoutina, G., Ray, C., Lametsch, R., et al. (2017) · International Dairy Journal · doi:10.1016/j.idairyj.2017.09.001 · Cited by 29 · see citing works
Mackie, A. R., & Rigby, N. M. (2015) · Springer eBooks · doi:10.1007/978-3-319-16104-4_2 · Cited by 27 · see citing works
Influence of culture conditions for clinically isolated non-albicans Candida biofilm formation
Tan, Y., Leonhard, M., Ma, S., & Schneider‐Stickler, B. (2016) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2016.09.011 · Cited by 27 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
The Potential Impact of Probiotics on Human Health: An Update on Their Health-Promoting Properties
Maftei, N.-M., Raileanu, C. R., Balta, A. A., Ambrose, L., Boev, M., Marin, D. B., & Lisa, E. L. (2024) · Microorganisms · doi:10.3390/microorganisms12020234 · Cited by 381 · see citing works
Probiotics in the dairy industry—Advances and opportunities
Gao, J., Li, X., Zhang, G., Sadiq, F. A., Simal‐Gandara, J., Xiao, J., & Sang, Y. (2021) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/1541-4337.12755 · Cited by 195 · see citing works
Barzegar, H., Alizadeh Behbahani, B., & Falah, F. (2021) · Food Science & Nutrition · doi:10.1002/fsn3.2365 · Cited by 108 · see citing works
Kim, E., Yang, S.-M., Lim, B., Park, S. H., Rackerby, B., & Kim, H.-Y. (2020) · BMC Microbiology · doi:10.1186/s12866-020-01781-z · Cited by 98 · see citing works
Patrignani, F., D'Alessandro, M., Vannini, L., & Lanciotti, R. (2020) · Sustainability of the Food System · doi:10.1016/b978-0-12-818293-2.00009-4 · Cited by 19 · see citing works
Self-citation
- Authors
- Patrignani, F., Siroli, L., Parolin, C., Serrazanetti, D. I., Vitali, B., & Lanciotti, R.
- Affiliations
- University of Bologna (IT)
- Corresponding author
- Patrignani, Francesca
- ORCID
- Venue
- PLOS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2019-01-09
- Volume
- 14
- Issue
- 1
- Pages
- e0208906
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC6326422
- OpenAlex
- W2909070987
- Cited by
- 51
- References
- 68
- Citations by year
- 2026: 4 · 2025: 7 · 2024: 5 · 2023: 7 · 2022: 8 · 2021: 8 · 2020: 9 · 2019: 3
- FWCI
- 5.40
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Archivio istituzionale della ricerca (Alma Mater Studiorum Università di Bologna) (submittedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Università di Bologna (The research was funded by the grant obtained within the Alma Idea Project “Approcci biotecnologici per la produzione di un alimento di genere - Biotechnological approaches to produce a gender food”, University of Bologna, Italy.)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Life Sciences › Agricultural and Biological Sciences › Food Science › Advanced Scientific Research Methods
- Keywords
- Lactobacillus crispatus, probiotic cheese, gynecologic infections, vaginal microbiota, antimicrobial activity, probiotic viability, SHIME, cold storage, proteolysis, lipolysis, volatile compounds, cheese acceptability, women's health
- MeSH terms
- Cheese, Female, Food Microbiology, Humans, Hydrogen-Ion Concentration, Intestines, Lactobacillus, Vagina, Ecosystem, Probiotics
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
