Differential survival of Cajal-Retzius cells in organotypic cultures of hippocampus and neocortex
Open accessAbstract
Cajal–Retzius (CR) cells are transient, pioneer neurons of layer I of the cortex that are believed to play essential roles in corticogenesis, e.g., in neuronal migration and synaptogenesis. Here we have used calretinin immunostaining to study the characteristics, survival, and fate of CR cells in single organotypic slice cultures of mouse neocortex and hippocampus deprived of their extrinsic afferents. In neocortical explants, CR cells were observed after 1–3 d in vitro(DIV), but they disappeared after 5–7 DIV, which is similar to their time of degeneration in vivo. The disappearance of CR cells in neocortical slices was prevented by incubation with tetrodotoxin and the glutamate receptor antagonist 6-cyano-7-nitroquinoxaline-2,3,-dione but not by 2-amino-5-phosphonopentanoic acid, suggesting that neuronal activity and non-NMDA glutamate receptors may trigger CR cell death in the neocortex. In contrast to the situation in vivo, in which many hippocampal CR cells disappear at approximately the third postnatal week, CR cells survived in single hippocampal cultures after long incubation times (31 DIV), with their morphology essentially unaltered. In contrast, fewer CR cells were found when hippocampal slices were cocultured with explants from the entorhinal cortex. Because CR cells are transient synaptic targets for entorhinohippocampal afferents, these findings suggest a role for entorhinal afferents in the degeneration of CR cells in the hippocampus. In conclusion, this study shows different survival properties of CR cells in organotypic slice cultures of hippocampus and neocortex, and it suggests that different mechanisms are involved in the regulation of the process of naturally occurring CR cell death in the two cortical regions.
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Inhibitory GABAergic interneurons begin to express and secrete Reelin postnatally as Cajal-Retzius cells die out in the cerebral cortex and later in the hippocampus.
“At this point, inhibitory GABAergic interneurons begin to express and secrete Reelin (6) as the Cajal-Retzius cells begin to die out in the cerebral cortex and later in the hippocampus (7).”
From Reelin: Neurodevelopmental Architect and Homeostatic Regulator of Excitatory Synapses (2017) · cites this paper as [7]
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Reelin: Neurodevelopmental Architect and Homeostatic Regulator of Excitatory Synapses
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“At this point, inhibitory GABAergic interneurons begin to express and secrete Reelin (6) as the Cajal-Retzius cells begin to die out in the cerebral cortex and later in the hippocampus (7).”
BackgroundA Novel Type of GABAergic Interneuron Connecting the Input and the Output Regions of the Hippocampus
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- Authors
- Del Rı́o, J. A., Heimrich, B., Supèr, H., Borrell, V., Frotscher, M., & Soriano, E.
- Affiliations
- University of Freiburg (DE)
- Universitat de Barcelona (ES)
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 1996-11-01
- Volume
- 16
- Issue
- 21
- Pages
- 6896-6907
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC6579265
- OpenAlex
- W2148478579
- Cited by
- 133
- References
- 80
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 1 · 2023: 2 · 2022: 3 · 2021: 4 · 2020: 5 · 2019: 4 · 2018: 4 · 2017: 4 · 2016: 6 · 2015: 3 · 2014: 2 · 2013: 4 · 2012: 4
- FWCI
- 3.86
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- FreiDok plus (Universitätsbibliothek Freiburg) (submittedVersion)
- Funders
- Deutsche Forschungsgemeinschaft (SFB 505)
- Leibniz-Gemeinschaft
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Keywords
- Cajal-Retzius cells, neuronal survival, neuronal cell death, organotypic slice cultures, hippocampus, neocortex, entorhinal cortex, non-NMDA receptors, tetrodotoxin, calretinin, cortical development
- MeSH terms
- Afferent Pathways, Animals, Animals, Newborn, Cell Survival, Cells, Cultured, Cerebral Cortex, Hippocampus, Mice, Inbred Strains, Microscopy, Electron, Nerve Degeneration, Neurons, Organ Culture Techniques, Somatosensory Cortex, Time Factors, Cell Death, Mice
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