CoChat
Sign Up
Cited by 218Neurogenesis and neuroplasticity mechanismsNeuroscience and Neuropharmacology ResearchAxon Guidance and Neuronal Signaling

GABA receptor antagonists modulate postmitotic cell migration in slice cultures of embryonic rat cortex

Behar, T. N.·2000·Cerebral Cortex
DOI 10.1093/cercor/10.9.899
Open this paper in CoChat — free account

Abstract

Recent studies indicate that GABA acts as a chemoattractant during rat cortical histogenesis. In vivo, GABA localizes in appropriate locations for a chemoattractant, along migratory routes and near target destinations for migrating cortical neurons. In vitro, GABA induces dissociated embryonic cortical neurons to migrate. Here, embryonic rat cortical slices were cultured in the presence or absence of GABA receptor (GABA-R) antagonists to assess GABA's effects on neuronal migration in situ. Gestational day 18 (E18) cortical slices were incubated overnight in bromodeoxyuridine (BrdU)-containing medium to label ventricular zone (vz) cells as they underwent terminal mitosis. The slices were then cultured in BrdU-free medium with or without GABA-R antagonists. In control slices, most BrdU(+) cells were observed in the cortical plate (cp) after 48 h. In contrast, cultures maintained in either saclofen (a GABA(B)-R antagonist) or picrotoxin (a GABA(A/C)-R antagonist) had few BrdU-labeled cp cells. However, the effects of the two antagonists were distinct. In the picrotoxin-treated slices, nearly half of all BrdU(+) cells remained in the vz and subventricular zone (svz), whereas saclofen treatment resulted in an accumulation of BrdU(+) cells in the intermediate zone (iz). Bicuculline, a GABA(A)-R antagonist, did not block, but rather enhanced migration of BrdU(+) cells into the cp. These results provide evidence that picrotoxin-sensitive receptors promote the migration of vz/svz cells into the iz, while saclofen-sensitive receptors signal cells to migrate into the cp. Thus, as cortical cells differentiate, changing receptor expression appears to modulate migratory responses to GABA.

Integrity signals

Registry

Crossref · 10.1093/cercor/10.9.899

Cites

43 of 43 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • GABA influences neuronal migration in the embryonic cortex, interneuron settlement in the cortical plate, inhibitory synapse formation, and may influence pyramidal cell neurogenesis.

    “GABA has been shown to influence neuronal migration in the embryonic cortex (Behar et al., 2000; López-Bendito et al., 2003), the settlement of interneurons in the cortical plate (Bortone and Polleux, 2009), and the formation on inhibitory synapses (Oh et al., 2016), and may also influence the neurogenesis of pyramidal cells (Silva et al., 2018).”

    From Development and Functional Diversification of Cortical Interneurons (2018) · cites this paper as Behar et al., 2000

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Authors
Behar, T. N.
Affiliations
National Institutes of Health (US)
Corresponding author
Behar, T. N.
Venue
Cerebral Cortex
Publisher
Oxford University Press (OUP)
Published
2000-09-01
Volume
10
Issue
9
Pages
899-909
ISSN
  • 1047-3211 (ISSN-L)
  • 1460-2199
DOI
10.1093/cercor/10.9.899
OpenAlex
W2135435398
Cited by
218
References
43
Citations by year
2025: 2 · 2024: 6 · 2023: 7 · 2022: 6 · 2021: 6 · 2020: 6 · 2019: 3 · 2018: 4 · 2017: 10 · 2016: 6 · 2015: 6 · 2014: 7 · 2013: 17 · 2012: 10
FWCI
1.52
Type
journal article
Language
en
Open access
closed
Locations
  • Cerebral Cortex (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
Keywords
GABA receptor antagonists, neuronal migration, picrotoxin, saclofen, bicuculline, GABAB receptors, GABAA receptor, GABAC receptors, ventricular zone, subventricular zone, intermediate zone, cortical plate, BrdU labeling
MeSH terms
Animals, Baclofen, Bicuculline, Bromodeoxyuridine, Cell Movement, Cerebral Cortex, Chemotaxis, Female, gamma-Aminobutyric Acid, Glial Fibrillary Acidic Protein, Intermediate Filament Proteins, Mitosis, Nerve Tissue Proteins, Neuroglia, Neurons, Organ Culture Techniques, Picrotoxin, Pregnancy, Receptors, GABA-A, Cellular Senescence, GABA Antagonists, Rats, Nestin

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review