Cited by 302Zebrafish Biomedical Research ApplicationsNeuroscience and Neuropharmacology ResearchDevelopmental Biology and Gene Regulation

alx , a Zebrafish Homolog of Chx10 , Marks Ipsilateral Descending Excitatory Interneurons That Participate in the Regulation of Spinal Locomotor Circuits

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Kimura, Yukiko, Okamura, Yasushi, Higashijima, Shin-ichi·2006·The Journal of Neuroscience

Abstract

Recent molecular genetic studies suggest that the expression of transcription factors in the developing spinal cord helps determine the morphological and physiological properties of neurons. Using the zebrafish preparation, we have examined the properties of neurons marked by alx , a zebrafish homolog of mammalian Chx10 . We performed morphological and physiological studies using transgenic zebrafish expressing fluorescent reporter constructs in cells that had at any time point expressed alx ( alx neurons). Our data reveal that zebrafish alx neurons are all ipsilateral descending neurons that are positive for vesicular glutamate transporter 2, suggesting that they are glutamatergic excitatory interneurons. Patch recordings show that earlier-born neurons are active during stronger movements such as escapes and fast swimming (strong movement class), whereas later-born ones are involved in sustained weak swimming (weak movement class). Paired recordings between alx neurons and motoneurons show that neurons of the strong movement class make frequent monosynaptic excitatory connections onto motoneurons. Thus, neurons of this class are likely premotor interneurons that regulate motoneuron activity during escapes and fast swimming. We also show the existence of a monosynaptic connection between an alx neuron of the weak movement class and a motoneuron. Collectively, our data demonstrate that alx marks ipsilateral descending neurons that are involved in the regulation of motoneuron activity during forms of locomotion, such as escape and swimming.

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Crossref · 10.1523/jneurosci.4993-05.2006

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  • In R7/R8, the former VPNI cells have somata primarily within a medial glutamatergic parasagittal stripe associated with the Alx transcription factor, and the latter with a more lateral GABAergic parasagittal stripe associated with the Abx1b transcription factor.

    “In R7/R8, the former were found to have somata primarily within a medial glutamatergic parasagittal stripe associated with the Alx transcription factor and the latter with a more lateral GABAergic parasagittal stripe associated with the Abx1b transcription factor21,95,96.”

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Authors
Kimura, Y., Okamura, Y., & Higashijima, S.-I.
Affiliations
National Institutes of Natural Sciences (JP)
Venue
The Journal of Neuroscience
Published
2006-05-24
Volume
26
Issue
21
Pages
5684-5697
ISSN
  • 0270-6474 (ISSN-L)
  • 1529-2401
OpenAlex
W2030150624
Cited by
302
References
54
Citations by year
2026: 6 · 2025: 10 · 2024: 9 · 2023: 17 · 2022: 16 · 2021: 18 · 2020: 15 · 2019: 18 · 2018: 11 · 2017: 12 · 2016: 17 · 2015: 13 · 2014: 19 · 2013: 13 · 2012: 13
FWCI
6.77
Type
journal article
Language
en
Open access
Locations
  • Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords
spinal interneurons, vesicular glutamate transporter 2, spinal locomotor circuits, premotor interneurons, swimming behavior, escape behavior, zebrafish
MeSH terms
Animals, Efferent Pathways, Eye Proteins, Feedback, Interneurons, Locomotion, Nerve Net, Spinal Cord, Tissue Distribution, Zebrafish, Homeodomain Proteins, Excitatory Postsynaptic Potentials, Zebrafish Proteins

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