Cited by 74Neuroscience and Neuropharmacology ResearchNeural dynamics and brain functionNeurobiology and Insect Physiology Research

Electron Microscopic Reconstruction of Functionally Identified Cells in a Neural Integrator

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Vishwanathan, Ashwin, Daie, Kayvon, Ramirez, Alexandro D., Lichtman, Jeff W., Aksay, Emre R.F., Seung, H. Sebastian·2017·Current Biology

Abstract

Neural integrators are involved in a variety of sensorimotor and cognitive behaviors. The oculomotor system contains a simple example, a hindbrain neural circuit that takes velocity signals as inputs and temporally integrates them to control eye position. Here we investigated the structural underpinnings of temporal integration in the larval zebrafish by first identifying integrator neurons using two-photon calcium imaging and then reconstructing the same neurons through serial electron microscopic analysis. Integrator neurons were identified as those neurons with activities highly correlated with eye position during spontaneous eye movements. Three morphological classes of neurons were observed: ipsilaterally projecting neurons located medially, contralaterally projecting neurons located more laterally, and a population at the extreme lateral edge of the hindbrain for which we were not able to identify axons. Based on their somatic locations, we inferred that neurons with only ipsilaterally projecting axons are glutamatergic, whereas neurons with only contralaterally projecting axons are largely GABAergic. Dendritic and synaptic organization of the ipsilaterally projecting neurons suggests a broad sampling from inputs on the ipsilateral side. We also observed the first conclusive evidence of synapses between integrator neurons, which have long been hypothesized by recurrent network models of integration via positive feedback.

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Crossref · 10.1016/j.cub.2017.06.028

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Authors
Vishwanathan, A., Daie, K., Ramirez, A. D., Lichtman, J. W., Aksay, E. R. F., & Seung, H. S.
Affiliations
  • Princeton University (US)
  • Weill Cornell Medicine (US)
  • Harvard University (US)
Corresponding author
  • Vishwanathan, Ashwin
  • Seung, H. Sebastian
Venue
Current Biology
Publisher
Elsevier BV
Published
2017-07
Volume
27
Issue
14
Pages
2137-2147.e3
ISSN
0960-9822 (ISSN-L)
OpenAlex
W2735195016
Cited by
74
References
56
Citations by year
2026: 2 · 2025: 8 · 2024: 6 · 2023: 6 · 2022: 14 · 2021: 8 · 2020: 9 · 2019: 12 · 2018: 9
FWCI
4.02
Type
journal article
Language
en
Open access
Locations
  • Current Biology (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • NIH (EY007138-16, R01027036)
  • Burroughs Wellcome
  • Searle Scholars Program
  • Simons Collaboration on the Global Brain
  • NSF CRCNS (1208088)
  • Mathers Foundation
  • Gatsby Foundation
  • Human Frontier Science Program
  • NIH/NINDS (5R01NS076467)
  • ARO MURI (W911NF-12-1-0594)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
Keywords
neural integrator, temporal integration, oculomotor system, zebrafish larvae, hindbrain, eye position, spontaneous eye movements, serial electron microscopy, neuronal morphology, ipsilateral and contralateral projections, glutamatergic neurons, GABAergic neurons
MeSH terms
Animals, Axons, Eye Movements, Microscopy, Electron, Scanning, Neurons, Rhombencephalon, Zebrafish

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