Electron Microscopic Reconstruction of Functionally Identified Cells in a Neural Integrator
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Neural integrators are involved in a variety of sensorimotor and cognitive behaviors. The oculomotor system contains a simple example, a hindbrain neural circuit that takes velocity signals as inputs and temporally integrates them to control eye position. Here we investigated the structural underpinnings of temporal integration in the larval zebrafish by first identifying integrator neurons using two-photon calcium imaging and then reconstructing the same neurons through serial electron microscopic analysis. Integrator neurons were identified as those neurons with activities highly correlated with eye position during spontaneous eye movements. Three morphological classes of neurons were observed: ipsilaterally projecting neurons located medially, contralaterally projecting neurons located more laterally, and a population at the extreme lateral edge of the hindbrain for which we were not able to identify axons. Based on their somatic locations, we inferred that neurons with only ipsilaterally projecting axons are glutamatergic, whereas neurons with only contralaterally projecting axons are largely GABAergic. Dendritic and synaptic organization of the ipsilaterally projecting neurons suggests a broad sampling from inputs on the ipsilateral side. We also observed the first conclusive evidence of synapses between integrator neurons, which have long been hypothesized by recurrent network models of integration via positive feedback.
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ABDM axons exited R5 and R6 through the ABD (VIth) nerve.
“ABDM axons exited R5 and R6 through the ABD (VIth) nerve (Fig. 1f, black box) as reported previously22.”
From Predicting modular functions and neural coding of behavior from a synaptic wiring diagram (2024) · cites this paper as [22]
The Results section on axonal projection patterns places the abducens motor nucleus within rhombomeres r5 and r6. It does not say that abducens motor-neuron (ABDM) axons exit those rhombomeres through the VIth nerve. (LLM, conf=0.76)
Experimental — accuracy not yet measuredThe imaged volume includes neurons involved in eye movements.
“The imaged volume includes neurons involved in eye movements16,21,22,28.”
From Predicting modular functions and neural coding of behavior from a synaptic wiring diagram (2024) · cites this paper as [22]
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Zebrafish as a Model for Revealing the Neuronal Basis of Behavior
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- Authors
- Vishwanathan, A., Daie, K., Ramirez, A. D., Lichtman, J. W., Aksay, E. R. F., & Seung, H. S.
- Affiliations
- Princeton University (US)
- Weill Cornell Medicine (US)
- Harvard University (US)
- Corresponding author
- Vishwanathan, Ashwin
- Seung, H. Sebastian
- Venue
- Current Biology
- Publisher
- Elsevier BV
- Published
- 2017-07
- Volume
- 27
- Issue
- 14
- Pages
- 2137-2147.e3
- ISSN
- 0960-9822 (ISSN-L)
- PMC
- PMC5569574
- OpenAlex
- W2735195016
- Cited by
- 74
- References
- 56
- Citations by year
- 2026: 2 · 2025: 8 · 2024: 6 · 2023: 6 · 2022: 14 · 2021: 8 · 2020: 9 · 2019: 12 · 2018: 9
- FWCI
- 4.02
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Current Biology (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- NIH (EY007138-16, R01027036)
- Burroughs Wellcome
- Searle Scholars Program
- Simons Collaboration on the Global Brain
- NSF CRCNS (1208088)
- Mathers Foundation
- Gatsby Foundation
- Human Frontier Science Program
- NIH/NINDS (5R01NS076467)
- ARO MURI (W911NF-12-1-0594)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Keywords
- neural integrator, temporal integration, oculomotor system, zebrafish larvae, hindbrain, eye position, spontaneous eye movements, serial electron microscopy, neuronal morphology, ipsilateral and contralateral projections, glutamatergic neurons, GABAergic neurons
- MeSH terms
- Animals, Axons, Eye Movements, Microscopy, Electron, Scanning, Neurons, Rhombencephalon, Zebrafish
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