The DOI matches its Crossref record; 63 of the 63 references it cites were found in a registry or an index.
In vitro reconstitution of a highly processive recombinant human dynein complex
Open accessAbstract
Cytoplasmic dynein is an approximately 1.4 MDa multi‐protein complex that transports many cellular cargoes towards the minus ends of microtubules. Several in vitro studies of mammalian dynein have suggested that individual motors are not robustly processive, raising questions about how dynein‐associated cargoes can move over long distances in cells. Here, we report the production of a fully recombinant human dynein complex from a single baculovirus in insect cells. Individual complexes very rarely show directional movement in vitro . However, addition of dynactin together with the N‐terminal region of the cargo adaptor BICD 2 ( BICD 2N) gives rise to unidirectional dynein movement over remarkably long distances. Single‐molecule fluorescence microscopy provides evidence that BICD 2N and dynactin stimulate processivity by regulating individual dynein complexes, rather than by promoting oligomerisation of the motor complex. Negative stain electron microscopy reveals the dynein–dynactin– BICD 2N complex to be well ordered, with dynactin positioned approximately along the length of the dynein tail. Collectively, our results provide insight into a novel mechanism for coordinating cargo binding with long‐distance motor movement.
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Dynactin increases the processivity of the cytoplasmic dynein motor
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Self-citationKinesin and Dynein Move a Peroxisome in Vivo: A Tug-of-War or Coordinated Movement?
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Self-citationThe structure of the dynactin complex and its interaction with dynein
Urnavicius, L., Zhang, K., Diamant, A. G., Motz, C., Schlager, M. A., Yu, M., Patel, N. A., Robinson, C. V., & Carter, A. P. (2015) · Science · doi:10.1126/science.aaa4080 · Cited by 421
Self-citationAxonal transport: Driving synaptic function
Guedes-Dias, P., & Holzbaur, E. L. F. (2019) · Science · doi:10.1126/science.aaw9997 · Cited by 355
Cryo-EM shows how dynactin recruits two dyneins for faster movement
Urnavicius, L., Lau, C. K., Elshenawy, M. M., Morales-Rios, E., Motz, C., Yildiz, A., & Carter, A. P. (2018) · Nature · doi:10.1038/nature25462 · Cited by 283
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Metadata
- Authors
- Schlager, M. A., Hoang, H. T., Urnavicius, L., Bullock, S. L., & Carter, A. P.
- Venue
- The EMBO Journal
- Published
- 2014-07
- Volume
- 33
- Issue
- 17
- Pages
- 1855-1868
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- OpenAlex
- W2099485650
- Cited by
- 351
- References
- 63
- FWCI
- 12.33
- Type
- journal article
- Language
- en
- Open access
- hybrid
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